Shokubutsugaku Zasshi

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The number and shape of nuclei in the spores of Streptomyces which belong to B and C groups were observed. The results were as follows:
1. On B group.
1) Seven strains out of thirteen most spores were uninucleate. The shape of nucleus seems spherical or ellipsoidal.
2) In four strains out of thirteen, S. lavendulae ATCC-8664 (17%), S. erythrochromogenes W-115C (8%), S. pheochromogenus W-41 (3%) and S. cinnamonensis 154-T3 (rarely), two nuclei were observed.
3) Trinucleate spores were rarely observed only in S. lavendulae ATCC-8664.
4) Specially large nucleus was observed in S. pheochromogenus W-41.
5) Nucleus could not be observed in two strains of S. roseochromogenus.
2. On C group.
1) On twelve strains out of eighteen most spores were uninucleate. The shape of nucleus seems spherical or ellipsoidal.
2) In six strains out of eighteen, that is, S. olivaceus NRRL-B-1125 (12 %); S. microflavus 13-A (8%), S. sahachiroi (5%), S. olivaceus CR-74 (1.5%), S. griseus #1 (rarely) and S. griseus MC-33 (rarely), binucleate spores were observed.
3) Trinucleate spores were rarely observed only in S. olivaceus NRRL-B-1125.
4) Ring nucleus was observed in S. olivaceus GSM^r₅₀₀₀
5) It was observed that there were so many none-nucleate spores in S. olivaceus CR-74.

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In the common garden varieties of Swiss Giant pancy, Viola Wittrockiana Gams, the flower colors ranging from red to blue are caused by at least six anthocyanins. They were separated from each other by mass paper chromatography in comparatively pure state. Their component sugar, organic acid, etc. were studied chromatographically after complete as well as partial hydrolysis, and also after saponification with alkali. The results show that the major pigment is keracyanin in reddisch varieties, whereas the pigment in bluish varieties is not violanin (delphinidin-3-p-coumarylglucorhamnoside) but a new anthocyanin, probably having a constitution of delphinidin-3: 5-p-coumarylglucorhamnoside. Among four kinds of minor constituent, two are shown to be tulipanin and cyanidin-3:5-glucoglucorhamnoside, and the remaining two are deriva tives of cyanidin and delphinidin.

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