The study on the chimeras has been carried out in several species of Solanaceae. The plants used as the scion are of 3 species, i.e. S. nigrum, S. villosum and L. pimpinellifolium, and L. esculentum was alone taken as the stock. Of these combinations, the case of S. nigrum is exclusively reported in this paper. It was indicated from the results obtained that 1) the chimera plants showed intermediate characters in several structures, the cell size of leaf epidermis, hair styles on leaf surface, flower size, number of petals and sepals, fruit weight etc., 2) the pollen sterility of the chimeras was considerably high and the seeds in the ripe fruits from the chimera were almost defective, resembling the facts previously reported by Winkler. It was clearly that, from the chromosome analysis, these chimeras belonged to S. Gaertnerianum and S. Koelreuterianum named by Winkler. Especially in one case, the 4x tomato-stem was surrounded by one layer of S. nigrum. From these phenomena it was considered that a chimera was one of the graft hybrids. It seems to the author that these results surely support the opinion of Glushchenko.
Based on the observations concerning the occurrence of a group of anthraquinones in the mycelia of Penicillium islandicum Sopp., NRRL 1036 during cultivation, the biosynthetic relationship among individual components was discussed. The present experiments have shown that the Shibata's hypothetical biosynthetic sequence (Fig. 1) holds good only with some modifications as follows: Iridoskyrin was always formed in the mycelia prior to the appearance of luteoskyrin (cf. Tab. 3). Iridoskyrin seems, therefore, to be derived neither from rubroskyrin nor luteoskyrin, but presumably from islandicin (Fig. 1) because of the fact that almost simultaneous appearance and disappearance could be observed between iridoskyrin and islandicin even after successive inoculation on the minimal media, whereas such a parallelism was not observed in the case of iridoskyrin and rubroskyrin. Finally, it may be noted that the Spot-i (designated by Shibata et al., 1955) was identified as oxyskyrin by paper chromatographic method (Tab. 2).