Taxonomy of several species complexes was discussed on the basis of the examination of specimens preserved in various herbaria in Europe, the United States and Japan. The following results were obtained. (1) The wild plants belonging to O. sativa complex should be divided into two species, O. rufipogon and O. barthii. O. rufipogon includes the Asiatic and American plants, and O. barthii comprises the African plants. O. madagascariensis is identical with O. barthii and should be referred to the latter as a synonym. (2) O. stapfii is most reasonably referred to O. breviligulata as a synonym. (3) O. longiglumis is distinguished from O. ridleyi in the combination of several morphological characters and is maintained as a separate species. (4) O. granulata is connected with O. meyeriana by a considerable number of intermediates. Eight types have been recognized in this species complex, and they should be brought into one species, O. meyeriana. Subdivision of O. meyeriana into three subspecies has been proposed, and some nomenclatural transfer was given.
1. Water soluble chlorophyllase preparation was made from chloroplasts of tobacco plants, using n-butanol and aqueous solution of sodium chloride as the solvents. 2. Some properties of the enzyme were studied. 3. Seasonal changes of enzyme activity and chlorophyll content were studied. Chlorophyllase activity varied in advance of the variation of chlorophyll content. 4. Possible participation of chlorophyllase in the biosynthesis of chlorophyll was. discussed.
(1) If normal eggs of Coccophora Langsdorfii or Fucus evanescens are immersed in 1.6 to 2.0M CaCl2 solution at a stage after their polarity was determined morphologically, dark cytolysis occurs at first in the prospective rhizoid region and then it spreads to the whole region. In Fucus eggs, abnomally, sometimes two or three primary rhizoids are formed. In these eggs, the cytolysis is observed at each region in coincidence with the rhizoid bulgings. If Coccophora eggs are centrifuged at 1, 700 times gravity or higher for one minute and then immersed in the above solution, the cytolysis takes place at the centrifugal end regardless of the prospective rhizoid pole. (2) It seems that the regional cytolysis with CaCl2 in normal eggs can be attributed to gradients in susceptibility in the cortex of the egg cell for excessive Ca ions, highest at the rhizoid region and they are movable with centrifuging. Nevertheless, the developmental polarity is not always modified.