As already described in one of the previous publications of this series, four subfamilies of Gramineae were found to differ from each other in their amino acid pattern of the seeds.
This paper reports the results of the extension of the investigation of the same line, covering the seeds of twenty-three species belonging to four subfamilies: Arundinoideae, which was not treated in the former work, Pooideae, Eragrostoideae and Panicoideae. For amino acid determination microbiological assay technique was applied.
The seeds of Pooideae, Eragrostoideae and Panicoideae studied in this investigation showed the similar results to those reported previously in their characters in amino acid pattern. The seeds of Panicoideae showed an amino acid pattern characterized by high alanine and leucine contents in marked contrast to those of other three subfamilies, and also showed lower lysine and arginine contents as well as those of Eragrostoideae when compared with other two subfamilies. The seeds of Eragrostoideae showed alanine and leucine contents ranging between Pooideae and Panicoideae.
The seeds of Arundinoideae gave such an amino acid pattern as revealed in Pharoideae reported previously,
i.e. higher aspartic acid and arginine contents, and lower proline contents as compared with other subfamilies, however, one species of Arundinoideae,
Aristida uniplumis, showed a pattern similar to Eragrostoideae.
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