1) α-Amylase activity can be detected in the seedlings of Dianthus barbatus. 2) A slight α-amylase activity is observed in the flower of Triticum aestivum. About a week after anthesis, the activity attains its maximum value which is nearly as much as the value seen in seedings 2 days after sowing at 15°. 3) Formation of α-amylase in the seedlings of Dianthus barbatus and Triticum aestivum is inhibited by light in the early stage of germination at lower temperatures 20° in the former plant and 15° in the latter. Formation of α-amylase in these seedlings is then noticeably promoted by light on the 4th or 5th day after sowing, so that there may be some intermediate stage in which the formation is not affected by light at all.
1) The chromosome numbers and karyotypes were studied in eight species, one variety, and two subspecies belonging to Subfamily Carduoideae (Compositae) of Japan. 2) The numbers of somatic chromosomes were counted in two species of Gnaphalium, two subspecies of Anaphalis, and one variety of Leontopodium for the first time. 3) The basic numbers of chromosomes were found to be 7 in Gnaphalium, and 14 in Anaphalis. Leontopodium has 13 chromosomes which are derived from 14. 4) All the members of the Gnaphaliineae studied, except G. japonicum, G. purpureum (4n) and L. hayachinense (8n), have diploid forms in the karyotype. 5) A karyological similarity was found between A. margaritacea subsp. angustior and A. margaritacea subsp. yedoensis, and between A. sinica and A. alpicola respectively. 6) The genera Gnaphalium, Anaphalis and Leontopodium were discussed from the karyotypical and plant geographical stand points.