1.The optimum concentration of
Lupinus diffusate, which contanis growth-promoting factor for young
Datura embryo
in vitro, is equivalent to 10g of
Lupinus seed/100ml of the medium.
The growth-promoting factor in the
Lupinus diffusate is not destroyed by autoclaving at 1.0kg/cm
2 overpressure for ten minutes.
The
Lupinus diffusate promotes remarkably the growth of young
Datura embryos, but not the old ones.
2. By
Lupinus diffusate, the growth of embryos of
Pharbitis nil, Bidens biternata,
Brassica campestris, Lupinus luteus, Capsella bursa-pastoris and of hybrid embryos from a genetically incompatible cross of
Brasssca pekinensis×B. chinensis are promoted remarkably. This diffusate is slightly effective for the growth of embryos of
Stellaria media, Antirrhinum majus, Astragalus sinicus, and
Triticum aestivum, nearly ineffective to that of
Vicia faba and
V. sativa, and inhibitory to
Iris pseudoacorus.
3. In
Stellaria and
Triticum, the
Lupinus diffusate supports embryonal growth and prevents precocious germination.
4. The
Lupinus diffusate promoted the growth of cultured callus tissue of carrot root.
5. Similar embryo factor activity was found in the ethanol diffusates of seeds of
Glycine soja, Phaseolus vulgaris var.
humilis and “Black Valentine”.
Arachis hypogaea,
Pisum sativum, Canavalia ensiformis and of fruits of
Brassica campestris.
View full abstract