Spores of
Equisetum arvense, Osmunda japonica, Pteris vittata, and
Dryopteris varia were sown in Knop's solutions containing β-mercaptoethanol, cysteine, bal, reduced glutathione, oxidized glutathione, cystine, dithiodiglycolic acid, and colchicine, and were incubated under constant illumination of white light at 1, 200lux. The results were as follows. (1) Elongation of the rhizoid was variously inhibited by mercaptoethanol, bal and by reduced glutathione, but not by cystine, oxidized glutathione and by dithiodiglycolic acid. Protonema cells were scarcely affected by any of these agents. Effect of colchicine was not very remarkable for rhizoids. (2) Protonema cell was hypertrophied with colchicine, but not or scarcely by SH- or SS-containing agents so far as tested. (3) Judging from the experiments, it seems that the cellular polarity is based on SS-linkage of proteins both in the rhizoid and in the protonema. So that, it is obstructed by SH groups or by colchicine, while not affected by SS groups. The specific protein or the state of protein may be different in rhizoid from in protonema, as the former seems to be affected by SH groups but scarcely by colchicine, while the latter by colchicine but scarcely by SH groups.
View full abstract