The relationship between GA (gibberellic acid)-induced growth and nucleic acid and protein metabolism was examined using plumular hook sections of etiolated pea seedling.
1. RNA (ribonucleic acid) content per initial fresh weight considerably decreases during the incubation period and the decrease is promoted with the increasing concentrations of GA. DNA (deoxyribonucleic acid) content per initial fresh weight hardly changes during the incubation period and it is increased only slightly with GA treatment. Protein content per initial fresh weight decreases during the incubation period and it is hardly affected by GA.
2. In cortex GA promotes only slightly the increases in the cell number only in the longitudinal direction during the incubation period.
3. Chloramphenicol and mitomycin C are effective inhibitors on the increase in fresh weight of both untreated and GA-treated sections. The protein and nucleic acid content is declined with the same concentrations of the inhibitors as effective on the growth during the incubation period in both cases.
4. The incorporation of the radioactivity from GA into s-RNA (soluble ribonucleic acid) which is concerned with the GA action was not demonstrated during the relatively early period of incubation.
It is concluded that there is some connection between GA-induced growth and nucleic acid and protein metabolism, although the mechanism of GA action in this system remains to be proved.
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