In the present investigation, the negative heteropycnotic satellites attached to the distal ends of the short arms of the shortest pair of chromosomes in Narcissus jonquilla L. were studied by the application of H3-thymidine autoradiography. It was confirmed that each of the two satellites, which show negative heteropycnosis at metaphase, forms each nucleolinus within the nucleolus at interphase. It was also observed that the satellites begin the DNA synthesis at mid-S phase and terminate at late S phase, before completion of DNA synthesis in another part of the chromosome.
Vegetative thalli of Vaucheria dichotoma and Vaucheria geminata were studied for their pigment content. Chromatography and spectral absorption characteristics of the pigments from those 2 species revealed the presence of chlorophyll c as a minor fraction, in addition to chlorophyll a, β-carotene and 3 xanthophylls. The fact of the presence of chlorophyll c in the genus Vaucheria offers a useful clue for discussing its systematic relationship.
A study of pyrenoid distribution in field material of 52 species of brown algae was conducted with an electron microscope. Of the representatives examined, 17 species possessed pyrenoids, while the rest lacked pyrenoids. It is interesting to note that most of the species possessing pyrenoids fall within the group of either isogamy or anisogamy, while those with lack pyrenoids fall within the group of oögamy.
A new genus, Congregatocarpus, is established on the basis of Laingia pacifica Yamada, a species that has those characters of both the Delesserieae and of Nitophylleae of the Delesseriaceae. That is, existing of intercalary division in the primary cell-row ; branching from the midrib ; formation of procarps on the midrib of the proliferations ; and formation of tetrasporangia by surface cells, etc.
This paper deals with the results of karyological studies made on fourteen moss species belonging to the Family Sematophyllaceae, Hypnaceae and Hylocomiaceae. In the mitotic cells of gametophytes the metaphase, anaphase and heteropycnotic chromosomes were observed. Chromosome numbers of the three species, Hypnum plumaeforme var. minus, Isopterygium seligeri and Gollania ruginosa, are reported for the first time by the author. However, those of the two species, Acroporium flagelliferum and Taxiphyllum taxirameum, are different from those reported by previous investigators. Furthermore, comparing among these karyotypes, the interspecific and intergeneric relations between them, and the aberrations of H- and/or h-chromosome in the course of the karyotypical evolution have been discussed. The karyotypes of these fourteen species are represented as follows: Sematophyllaceae Heterophyllium haldanianum K(n)=11=V(H)+2V+3J+4(3v+j)+m(h) Acroporium flagelliferum K(n)=10=V(H)+2V+3J+3(3v+j)+m(h) Hypnaceae Hypnum cupressiforme K(n)=10=V(H)+3V+2J+3(2v+j)+m(h) H. oldhamii K(n)=6=V(H)+2V+2J+v(h) H. plumaeforme var. minus K(n)=10=V(H)+3V+2J+(2v+j)+m(h) Isopterygium seligeri K(n)=11=2V(H)+2V+2J+4(2v+2j)+v(h) I. pohliacarpum K(n)=12=2V(H)+2V+2J+4(2v+2j)+2m(h) Taxiphyllum taxirameum K(n)=12=2V(H)+V+J+6(3v+3j)+2m(h) Ctenidium capillifolium K(n)=11=V(H)+2V+2J+5(3v+2j)+m(h) Rhytidiadelphus squarrosus K(n)=10=V(H)+3V+3J+2(v+j)+(h) R. triquestrus K(n)=6=V(H)+2V+2J+v(h) Gollania ruginosa K(n)=10=V(H)+3V+3J+2(v+j)+m(h) Hylocomiaceae Hylocomium brevirostre var. cavifolium K(n)=6=V(H)+2V+2J+j(h) H. pyrenaicum K(n)=12=2V(H)+3V+3J+2(v+j)+2m(h)
The spherical blepharoplast originates from the nucleus of spermatid in the spermatogenesis ofPteris multifida, Notogramme japonicaandIsoetes japonica. In MarsiliaandEquisetum, the centrosome of dividing spermatogenous cells remains in the produced spermatid and develops border-brim, cilia-bearing band and cilia. So one and the same double structure acts as centrosome in some cases, and acts as blepharoplast in the other cases. The double nature of blepharoplast has already been clarified in spermatogenesis of some animals, and the double nature of basal body which is thought to be the primitive type of blepharoplast has been confirmed in the division of zoospore ofStemonitis flavogenitaandS. splendensvar.flaccida. In this case the basal body also originates from the nucleus. In some cases, however, the basal body of zoospore showed positive Feulgen's reaction inStemonitisandFuligo. The information is sent out from the nucleus to blepharoplast or the basal body which develops the flagellar apparatus and controls the behavour of cilium or flagellum. The blepharoplast or basal body is thought to be the self-replicating system.