To make rheological and biological evaluation of dental materials, we measured the depth of cure
Dc and the surviving fraction
Sf of
HeLa cells cultured in extracted culture media from four resins:
Estio LC and
Graft LC in GC Co.,
Silux Plus in 3M Co., and
Palfique Light in Tokuyama Co.
Dc was determined from the rigid depth of the resins (6mm∅ × 8mm) polymerized with a plane light source (λ=462~478nm, 20sec).
Sf was defined as the ratio between active cell numbers in test and control media; active cell numbers were measured by OD at 570nm with MTT assay as a function of the resin surface concentration
C in media and the irradiation distance
L inside the resins. The results were as follows, 1)
Sf versus
L was represented by a logistic equation 2)
Sf decreased with the increase of
C and
L 3)
Sf most changed at
L near
Dc 4)
Dc was greater for
Estio and
Graft LC's with the higher content and larger size of fillers than
Silux Plus. It is suggested that cultured cells may be damaged by residual monomers.
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