In oder to ascertain the extent and distribution of two major mitochondrial DNA (mtDNA) haplotypes, A and B, in four European sheep breeds and four local Asian sheep breeds, the
Hinf I restriction fragment length polymorphism of mtDNA genomes was assayed using PCR techniques. In the European sheep group, Suffolk and Cheviot sheep had a conspicuously high frequency of type B (88 and 100%, respectively). However, in both of Corriedale and Finnish Landrace sheep, type A was seen at comparatively high frequency of about 40%, accompanied by a high frequency of type B. On the other hand, in the Asian sheep group, the frequency of type A was considerably high in Kagi and Lampuchhre sheep belonging to the Indian sheep group (95% and 96%, respectively). As for the Tibetan sheep group, Baruwal sheep had type A haplotypes at the frequency of 90%. However, 60% of Bhyanglung sheep were type A and 40% were type B, the frequency being different from those of the other local Asian breeds tested. The frequency constitution of the haplotypes varied among breeds. It was difficult to estimate their genetic relationships. However, from the level of global sheep groups such as European and Asian sheep, the average frequency of type B in the former was 71%, while the average frequency of type A in the latter was 85%. A marked difference between both sheep groups was seen. This finding, therefore, suggest that European and Asian sheep respectively consist chiefly of animals derived from different ancestoral maternal lineages.
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