In order to elucidate the phylogenetic relationship between Asian (Bengal, Kagi, Lampuchhre, Myanmar, Bhyanglung, Baruwal, and Khalkha) and European (Suffolk, Corriedale, Cheviot, and Finn) sheep breeds, clustering analysis and principal component analysis (PCA) were conducted. These methods were based on allele frequency data for six informative and polymorphic loci of blood protein and non-proteins, such as TF, ES, HB-β, XP, KE, and APOC, using different electrophoretic, immunobloting and ion-densitometric techniques. Based on NEIʼs genetic distance data, clustering analysis by the UPGMA method showed that the Asian sheep breed populations had diverged into northern (Bhyanglung, Baruwal, and Khalkha) and southern (Bengal, Kagi, Lampuchhre, Myanmar, ) Asian breed populations. Including British breed populations, all the sheep breed populations tested were broadly classified into three groups. In particular, the British group and the southern Asian group formed a large cluster. However, the Scandinavian Finn sheep was clustered in the northern Asian group and appeared to be closest to the Bhyanglung sheep, derived from Tibetan sheep. Clustering analysis by the NJ method and PCA showed similar results to those obtained by the UPGMA method. These findings, therefore, have verified that the breed populations tested are divided at least into three phylogenetic groups, but these cannot be separated completely and definitively into two groups of Asian and European sheep breed populations. This suggests that certain breeds among European sheep have a close relationship with Asian sheep breeds.
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