The Japanese Journal of Urology
Online ISSN : 1884-7110
Print ISSN : 0021-5287
ISSN-L : 0021-5287
Volume 81 , Issue 4
Showing 1-19 articles out of 19 articles from the selected issue
  • Tadao Kakizoe
    1990 Volume 81 Issue 4 Pages 501-517
    Published: April 20, 1990
    Released: July 23, 2010
    JOURNALS FREE ACCESS
    Reconstruction of the urinary tract was reviewed. Reconstruction of the ureter dealt with end to end ureteroureterostomy, transureteroureterostomy, bladder flap procedure, psoas hitch procedure, ureterovesiconeostomy, ileal inter position and autotransplantation of the kidney. Bladder augmentation concerned with use of the ileum, ileocecal segment and sigmoid colon, together with artificial material. Reconstruction of the bladder included urinary diversion such as ileal, jejunal, sigmoid, transverse and ileocecal conduit, ureterureterostomy, continent urinary reservoir such as Kock, Mainz and Indiana pouch, and total replacement of the bladder using various segments of intestine anastomosed to the urethra. Surgical endeavor performed by urologists during the past 100 years from 1890 to 1990 was tremendous and it was mainly reviewed from the standpoints of surgical technique and complications.
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  • Shinji Iwata, Hideyuki Isobe, Makoto Fujime, Ryuichi Kitagawa, Satoshi ...
    1990 Volume 81 Issue 4 Pages 518-523
    Published: April 20, 1990
    Released: July 23, 2010
    JOURNALS FREE ACCESS
    The concentration of laminin in the serum was determined for 45 patients with urinary tract transitional cell carcinoma, and the clinical significance of the findings was considered.
    The difference between the serum laminin concentrations in the control group and the transitional cell carcinoma patient group was not statistically significant, but the serum laminin level was highest in the transitional cell carcinoma patients with metastatic foci. In many of the patients who had metastatic foci and showed clearly progressive disease, the serum laminin concentration was found to increase with the passage of time. When the transitional cell carcinoma tissues were stained by the fluorescent antibody technique, there was little ditribution of laminin in the tumor tissues, contrary to our earlier-reported findings regarding the staining of laminin in renal cell carcinoma tissues. In renal cell carcinoma patients, the serum concentration of laminin had been found to be high even when there were no metastatic foci, and consideration of this fact strongly suggests the possibility that the mechanism for laminin synthesis in urinary tract transitional cell carcinoma is different from that in renal cell carcinoma.
    It was surmised that the serum laminin concentration has potential for use as a diagnostic indicator of metastasis in patients with urinary tract transitional cell carcinoma.
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  • Chikara Ohyama, Hisayoshi Nakazumi, Makoto Satoh, Kazuyuki Yoshikawa, ...
    1990 Volume 81 Issue 4 Pages 524-531
    Published: April 20, 1990
    Released: July 23, 2010
    JOURNALS FREE ACCESS
    At the time of operation, 10 patients with bladder tumors were given an intraveneous infusion of bromodeoxyuridine (BrdU, 250mg) to label the cells in the DNA synthesis phase (S-phase). The removed tumor specimens were stained immunohistologically using anti-BrdU monoclonal antibody. The percentage of BrdU-labeled cells (S-phase cells) were calculated for each specimen, and expressed as Labeling Index (LI).
    The average LI of G1, G2 and G3 tumors were 0.9%, 2.3% and 11.9%, respectively. LI was almost parallel to pathological grade. In cases of G2 tumor, LI was varied from 0.2 to 6.2%. The higher LI, the more invasive was the tumor. The patient with the highest LI (12.2%) showed lung metastasis postoperatively and died 6 months after operation.
    S-phase cells were scattered along the basal layer in G1 papillary tumors, and were observed also near the superficial layer in G2 tumors. In cases of non-papillary tumors, S-phase cells were observed irregularly in various layers. This method will give us usefull information on evaluation for malignancy and cell kinetics of bladder tumor.
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  • Toshikazu Okaneya, Akimi Ogawa
    1990 Volume 81 Issue 4 Pages 532-537
    Published: April 20, 1990
    Released: July 23, 2010
    JOURNALS FREE ACCESS
    Killer activity of peripheral blood mononuclear cells with or without adding interleukin-2 in vitro was measured in 12 patients with superficial bladder cancer, 12 patients with invasive bladder cancer and 13 adult healthy controls. Cultured cell lines, K562, Raji and T-24 (bladder carcinoma), were served as target cells. Killer activity was measured by a 4-hour 51Cr-release assay. Mononuclear cells from patients with superficial bladder cancer had a significantly higher natural killer activity against K562 and T-24 (50.8±6.2% and 15.0±9.5%, respectively) than those from patients with invasive bladder cancer (28.2±8.0%, 9.5±8.8%) and than those from controls (33.0±8.9%, 3.0±2.5%). When cultured in vitro with recombinant interleukin-2, mononuclear cells from patietns with superficial bladder cancer developed a significantly higher killer activity against K562, Raji and T-24 (58.4±5.8%, 40.1±15.9% and 49.5±10.5%, respectively) than those from patients with invasive bladder cancer (48.1±5.9%, 27.9±13.8% and 40.1±7.4%) and than those from controls (48.9±6.7%, 30.3±10.5% and 39.5±3.8%). Flow cytometric analysis showed that there was no significant difference in surface markers between mononuclear cells from patients with superficial bladder cancer and those from patients with invasive bladder cancer. These results suggest that tumor immunity may participate in development and progresion of bladder cancer.
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  • Shojiro Kanaya
    1990 Volume 81 Issue 4 Pages 538-545
    Published: April 20, 1990
    Released: July 23, 2010
    JOURNALS FREE ACCESS
    The intracellular contractile mechanism of the urinary bladder smooth muscle was studied using the saponin-treated skinned fiber in which cell membrane was chemically removed.
    The chemically skinned bladder muscle showed a tension development which was dependent on Ca2+-concentration. The minimal Ca2+-concentration for the tension development was 2×10-7M Ca2+. The maximal tension was induced at 10-5M. This maximal tension was approximately the same as the K2+-induced tension development observed in the intact muscle. In addition, SDS-polyacrylamide gel electrophoresis showed that the contractile proteins were still preserved in the saponin-treated bladder smooth muscle. The Ca2+-concentration-tension response curve shifted to the left with an increase in MgATP concentration (from 3mM to 7mM), indicating that the sensitivity of the skinned muscle was affected by MgATP.
    Mg2+ above 6mM caused a slow tension development by itself in the absence of Ca2+.
    Ca2+-induced tension development was blocked by the addition of W-7 (calmodulin antagonist).
    This result suggested that calmodulin (Ca2+-binding protein) regulates the actin-myosin interaction in the urinary bladder smooth muscle.
    Caffeine solution (25mM) caused a rapid tension development in the skinned bladder smooth muscle which was loaded with Ca2+-concentration, however, this tension development decreased when the loaded Ca2+-concentration exceeded 10-6M.
    It seems from this result that “Ca-induced-Ca release mechanism” also exists in the urinary bladder smooth muscle.
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  • Chiaki Irisawa
    1990 Volume 81 Issue 4 Pages 546-553
    Published: April 20, 1990
    Released: July 23, 2010
    JOURNALS FREE ACCESS
    To elucidate the change of hemodynamics of the penis during erection induced by neurostimulation to the plevic nerve, we measured the blood flow rates (BFR)through the dorsal penile artery and vein (DPA and DPV), and the internal pudendal artery (IPA)with the intracavernosal and intraspongiosal pressures (ICP and ISP). After DPA were ligated bilaterally, we compared BFR through DPV and ICP with those before ligations. In addition, PVO2 within DPV was determined.
    When neurostimulation was switched on, BFR through IPA and DPA began to increase. But during erection, BFR through IPA was lower than the flow in the tumescence phase, although the BFR through DPA remained unchanged. Then ISP elevated. When the full erection was achieved, the pressure remained above the prestimulation level, although a gradual decrease in the pressure was observed. On the other hand, ICP dropped transiently for 5 to 10 seconds, and then increased rapidly. BFR through DPV increased rapidly, which coincided with the transient drop in ICP. As soon as neurostimulation was switched off, BFR through IPA, DPA, DPV and ISP decreased to the base line, although ICP still remained elevated.
    The BFR through DPV and ICP were compared before and after ligation of bilateral DPA. BFR through DPV decreased after ligation during the non-erectile period, but no significant changes were found during the erectile period. ICP did not change significantly after ligation in either period. PVO2 within DPV was higher than that in the internal iliac vein and peripheral vein during both non-erectile and erectile period. In the erectile period, it rose up to a level near that within the aorta. These results suggest that there are two different conditins in the penis during erection, one is with a low flow, high pressure in the corpus cavernosum, and the other is with a high flow, low pressure in the corpus spongiosum.
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  • Motoyuki Yamashita, Keiji Inoue, Akemi Yamashita, Yukitoshi Fujita
    1990 Volume 81 Issue 4 Pages 554-560
    Published: April 20, 1990
    Released: July 23, 2010
    JOURNALS FREE ACCESS
    Intravesical instillation of anti-cancer drugs for superficial urinary bladder cancer is generally carried out with the aim of prophylaxis against recurrence and chemotherapy against tumor. But since sensitivity of tumor cells to each anti-cancer drug differs individually, the anti-cancer drug to be used should also be decided individually.
    We selected a new sensitivity test, ATP-senitivity-assay, which measures intracellular adeno-sintriphosphate (ATP) volume by Luciferin-Luciferase reaction, for the decision of the anti-cancer drug. In this paper, we evaluated the direct anti-tumor activity of the drugs that were decided by ATP-sensitivity-assay of intravesical chemotherapy. Six drugs, that are Doxorubicin (ADM), Mitomycin C (MMC), Pirarubicin (THP-ADM), Cytarabine (Ara-C), Bleomycin (BLM) and Cisplatin (CDDP) were entried in this research and size of tumors of six patients reduced to 34-93% after 6 times' instillation. A woman got cystitis induced by ADM after 3 times' instillation but instillation was completed.
    ATP-sensitivity-assay is useful for deciding the anti-cancer drugs for intravesical chemothrapy and prophylaxis for superificial bladder cancer.
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  • Toshiaki Gotoh, Michihiro Kubota, Yoshifumi Asano, Katsuya Nonomura, M ...
    1990 Volume 81 Issue 4 Pages 561-568
    Published: April 20, 1990
    Released: July 23, 2010
    JOURNALS FREE ACCESS
    By the recent advance in ultrasonography, an increasing number of patients with prenatally detected urological anomalies have been reported. Herein, we present 19 cases experienced in the last 3 years in our intitution and review the problems in their diagnosis and treatment.
    In all cases but 2 ultrasonography revealed fetal abnormalities after the 30th gestational week. No cases with abnormal amniotic fluid or severe fetal growth retardation were experienced. No cases were treated in utero. However, 7 cases required an early delivery because of progressive hydronephrosis or enlargement of tumor mass. Postnatally 6 cases were treated by emergency drainage such as percutaneous nephrostomy and placement of an indwelling catheter. However most of the cases with proven urinary tract dilatation were evaluated by diuretic renogram and followed conservatively thereafter. In equivocal cases, pressure-flow study was added. Spontaneous involution of multicystic kidney was also experienced. Erroneous interpretation can occur in fetal diagnosis and the accuracy of prenatal diagnosis was 52.6% in our series.
    In determining the management of prenatally detected urological anomalies, several factors such as reliability of ultrasonography, fetal renal function, maturity of the lung and the volume of amniotic fluid, must be taken into consideration. Considering the increasing number of fetuses thus detected, it is very important for the pediatric urologists to make consensus on the fetal intervention and postnatal management.
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  • Tetsuro Ohnishi, Toyohei Machida, Fujio Masuda, Norio Iizuka, Kenji Na ...
    1990 Volume 81 Issue 4 Pages 569-576
    Published: April 20, 1990
    Released: July 23, 2010
    JOURNALS FREE ACCESS
    Four hundred and thirty-one patients with renal cell carcinoma treated at Jikei University and its related institutions from 1957 to 1988 were studied as to tumour size in relation to clinical characteritics (age, sex ratio, affected side, symptoms), prognostic factor (fever, weight loss, anaemia, elevation of erythrocyte sedimentation rate), pathological staging, pathological grading, and survival. The treated patients were divided into 4 groups on the basis of tumour size (group A; under 3.0cm, group B; 3.1 to 5.9cm, group C; 6.0 to 9.9cm, group D; over 10cm in diameter). At the time of operation, there were 22 cases (5. 1%) in group A, 121 cases (28.1%) in group B, 209 cases (48.5%) in group C, and 79 cases (18.3%) in group D.
    In studying the clinical factors of age, sex ratio, and affected side, no relationship with size was detected. As for the symptoms, the larger the tumour, the more frequent the symptoms of urinary tract (haematuria, pain, and palpable mass). This tendency was especially marked in the cases of group D (over 10.0cm in diameter). In addition, no relationships were observed between the rate of haematuria and the symptoms of extraurinary tract in each group.
    As for the prognostic factors (fever, weight loss, anaemia and the elevation of erythrocyte sedimentation rate), there was an obvious tendency for a positive reaction of these factors in the cases of groups C and D (over 6.0cm in diameter).
    As for the distribution of stage and grade in each group, a higher stage and grade were observed with large sized tumours. Especially in stage IV B, cases of larger tumour size and higher grade predominated.
    Survival declined with increasing size of tumour, but no significant differences were observed between groups A and B, or groups C and D. When analysing survival in relation to stage and grade in group A, certain tumour characteristics were found to be associated with a poor prognosis, e. g., high grade cases. Thus, even with smaller sized tumours (under 3.0cm in diameter) attention must be paid to histological grade, and if the grade of tumour is high, postoperative powerful adjuvant therapy is needed to improve the prognosis for such cases.
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  • Hiroaki Masuda, Masataka Hirai, Yoshihisa Ohtawara, Atsushi Tajima, Yo ...
    1990 Volume 81 Issue 4 Pages 577-582
    Published: April 20, 1990
    Released: July 23, 2010
    JOURNALS FREE ACCESS
    Rats with bladder tumor induced by BBN were treated by intravesical instillation of 0.8mg of (2″R)-4′-O-tetrahydropyranyladriamycin (THP) (9rats) or adriamycin (ADM) (8rats) dissolved in 0.2ml of distilled water. Thirty minutes later, the bladder was removed surgically. Rats without the tumor received the same treatment of THP (8rats) or ADM (9rats). THP and ADM infiltrations to the normal bladder tissue and the tumor were estimated by the use of the photonic microscope system, since both drugs were known to emit characteristic fluorescence. It was found that infiltration of THP to the tumor tissue was more prominent in amounts and deeper than that of ADM, while smaller amounts of THP infiltrated into the normal mucosa compared to ADM. The fact might explain the clinical finding that THP instilled intravesically in half a concentration of ADM showed the same effect on the tumor as ADM.
    Subsequently, tissue concentrations of THP and ADM were estimated by high perfomance liquid chromatography. Either THP or ADM was instilled intravesically for 30 minutes to 6 rats with bladder tumor. Similarly, either THP or ADM was instilled in 5rats without the tumors. Contrary to the result of the photonic microscope system, the tissue concentration of THP was not different from that of ADM not only in the tumor tissues but also in the normal bladder ones. Furthermore, the tissue concentration of both drugs in the normal bladder was higher than that in the bladder tumor. The causes were considered as follows: since the amount of infiltrated drugs was very small in the face of the large amount of removed tissues, it was difficult to find the difference between both drugs. The weight of tumor tissues was so much larger than the normal bladder tissues that the tissue concentration of both drugs in the bladder tumor was low compared to that in the normal bladder.
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  • Shozo Ohta, Ryuichiro Konda, Kiyohide Sakai, Satoru Kuji, Seiichi Orik ...
    1990 Volume 81 Issue 4 Pages 583-588
    Published: April 20, 1990
    Released: July 23, 2010
    JOURNALS FREE ACCESS
    The function of 99 refluxing kidneys of 61 children up to two years old at the time of diagnosis was evaluated by 99mTc-DMSA renoscintigraphy, urinary β2-microglobulin (β2-MG), α1-microglobulin (α1-MG) and N-acetyl-β-D-glucosamidase (NAG).
    High grade reflex (grade IV, V) was found in 45% of the cases.
    76%, 51% and 92% of the cases showed abnormaly high value of urinary β2-MG, α1-MG and NAG, respectively. These results indicate that they have already had tubular dysfunction at the time of diagnosis.
    DMSA renoscintigraphy of 82 refluxing kidneys of 49 chirdren was performed. Of these, 48% had renal scar and 28% had serious renal dysfunction [DMSA uptake rate less than 18%]. 10-20% of low grade VUR had less than 18% DMSA uptake rate.
    Two patterns were noticed in the group in which urinary β2-MG ranged 0.33-1.0. One was characterized by good bilateral renal function with slight tubular damage and the other serious renal dysfunction with fixed tubular damage. No remarkable recovery of the renal function was noticed after antireflux operation in the second pattern cases. In many cases, in which urinary β2-MG was more than 1.0, improvement of DMSA uptake rate was noticed after antireflux operation.
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  • Wataru Sakamoto, Taketoshi Kishimoto, Keisuke Yamamoto, Toshikado Sugi ...
    1990 Volume 81 Issue 4 Pages 589-592
    Published: April 20, 1990
    Released: July 23, 2010
    JOURNALS FREE ACCESS
    Extracorporeal shook wave lithotripsy (ESWL) was performed for the treatment of 1277 urolithiasis patients at Osaka City University Hospital from July, 1985 to December, 1988. A total of 1788 ESWL treatments were carried out using Dornier HM3 kidney lithotripter. 964 patients (75.5%) underwent only one ESWL treatment, while 313 patients (24.5%) more than two ESWL treatments. We retrospectively examined the factors for requiring more than two ESWL treatments. Not only stone number, size and location, but fragility were considered to be the main causes for requiring more than two ESWL treatments. According to component analysis of ureteral stones, which were hard and resistant to shock wave, calcium apatite content of these stones turned out to be high (p<0.001). As for fragility, the residual stones created by ESWL were more difficult to be disintegrated that the nontreated stones of the same size (p<0.05).
    “Fragility” of the stones before shock wave and residual stones after ESWL are considered to be a major problem in ESWL treatment of urinary stones to be solved in the years to come.
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  • Satoshi Nagamori
    1990 Volume 81 Issue 4 Pages 593-600
    Published: April 20, 1990
    Released: July 23, 2010
    JOURNALS FREE ACCESS
    DNA content in renal cell carcinoma was investigated to examine the tumor heterogeneity and was correlated with their morphologic grades. A total number of 147 paraffin-embedded samples (2-6 samples with a mean of 5) from 30 tumors were analyzed by flow cytometry. DNA aneuploid patterns were demonstrated in 5 of 9 grade 1 tumors (56%), 15 of 18 grade 2 tumors (83%) and 3 of all grade 3 tumors (100%), while aneuploid DNA histograms were exhibited in 20 of 57 grade 1 samples (35%), 45 of 81 grade 2 samples (56%) and 8 of 9 grade 3 samples (89%). DNA aneuploid patterns were demonstrated more significantly in grade 2 and 3 samples than in grade 1 samples (p<0.02 and p<0.01, respectively). Consequently, 73 samples (50%) showed DNA aneuploid patterns and 74 samples exhibited DNA diploid patterns. Eleven tumors (37%) showed homogeneous DNA ploidy patterns (7 tumors were diploid and 4 tumors were aneuploid only), while 19 tumors (63%) showed DNA heterogeneity, 17 of these 19 tumors demonstrating diploid as well as aneuploid samples. DNA heterogeneity was not found when the tumor samples examined were fewer than 3 samples. However, 19 of 25 tumors (76%) in which more than 4 samples were examined showed DNA heterogeneity, the incidence of which tended to be increased with upgrading of the tomor-3 of 9 (33%) in grade 1 tumors, 13 of 18 (72%) in grade 2 tumors and 3 of all (100%) in grade 3 tumors. In correlation with DNA heterogeneity and morphologic heterogeneity, 13 of 17 tumors (76%) with morphologically heterogeneous grade also showed DNA heterogeneity, while only 6 of 13 tumors (46%) exhibiting morphologically homogeneous grade showed DNA heterogeneity. Eighty-nine samples from morphologic heterogeneous 17 tumors were divided into two groups. One was high grade group and the other was low grade group. Aneuploid samples were exhibited in 24 of 34 samples (71%) for the former group and 25 of 55 samples (45%) for the latter. Aneuploid samples were demonstrated significantly more frequently in the high grade group than in the low grade group (p<0.02).
    It appears that DNA heterogeneity is exhibited in more than half of renal cell carcinoma. The greater part of heterogeneous tumors have diploid as well as aneuploid samples. Sampling the paraffin-embedded materials, we must choose the part of the highest grade area in its tumor. DNA heterogeneity in renal cell carcinoma apears to be closely related to the numbers of tumor samples examined and their morphologic grades.
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  • Masaaki Tachibana, Seido Jitsukawa, Nobuhiro Deguchi, Shirou Baba, Mak ...
    1990 Volume 81 Issue 4 Pages 601-607
    Published: April 20, 1990
    Released: July 23, 2010
    JOURNALS FREE ACCESS
    The relationship between histopathological characteristics and their long-term prognosis was studied on a total of 110 patients with bladder cancer who underwent total cystectomy from 1978 to 1985 at Keio University Hospital in the period after computer tomography (CT) was initiated for the evaluation of tumor stagings.
    The actuarial five-year survival rate in the 110 patients estimated by Kaplan-Meier method was 54.3%. Disclosed as histological poor prognostic factors (p<0.05) were the presence of non-transitional cell elements (actuarial five-year survival rate: 26.3%), INFγ for growth patterns (26.3%), grade-3 for histological grades (39.1%), lymph-duct involvement ly(+) (28.2%), venous involvement v(+) (25.2%), muscle-invaded tumor and extravesical lymphnode involvement.
    As for the comparative relation among these histological factors, grade-3 tumors tended to have a high incidence of lymph-duct involvement (73.2%), venous involvement (26.8%) and positive lymphnodes (35.7%) when compared to grade-2 tumors. The analysis of the degree of influence of those histological prognostic factors using the non-proportional hazard model revealed that the major priorities were positive lymphnodes (p=0.0006), histological types (p=0.0036) and lymph-duct involvement (p=0.0839).
    These results suggest that intensive adjuvant treatment should be indicated for improving the prognosis of patients with bladder cancer who have undergone total cystectomy when non-transitional cell elements and/or vascular involvement are evidenced.
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  • Noriharu Mikata, Sadao Imao, Atsushi Katoh
    1990 Volume 81 Issue 4 Pages 608-613
    Published: April 20, 1990
    Released: July 23, 2010
    JOURNALS FREE ACCESS
    Stereographic reproduction of human organs, now under development as an application of computer graphics, aims at delineating the three-dimensional spread of a lesion, simulating an operation and drawing up a radiotherapeutic plan. We are currently attempting to obtain a stereo display of the kidney using a 3DCT method based on X-ray CT data obtained by scanning a 4mm slice in cases of bilateral renal calculus of the template type, renal bleeding and renal carcinoma.
    This 3DCT method was capable of displaying a stereo image of the kidneys relatively with ease since it made analysis possible, using a computer built inside the X-ray CT device.
    The development of a software program capable of stereographically displaying many organs on the same screen is an awaited future project.
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  • Masamichi Hagiwara, Masaaki Nakazono
    1990 Volume 81 Issue 4 Pages 614-617
    Published: April 20, 1990
    Released: July 23, 2010
    JOURNALS FREE ACCESS
    Eight patients underwent pouchmetry at 12 months after creation of the colonic continent urinary reservoir (CUR) for supravesical diversion. Pouchmetry revealed a volume-dependent tonic and phasic increase in pouch pressure in 3 patients who had undergone pouch construction using the partially detubularized right colon (Heineke-Mikulicz type closure after splitting the transverse and ascending colon at the antimesenteric border), whereas the volume-dependent phasic increase in pouch pressure was much less remarkable in 5 patients whose pouch was constructed using the totally detubularized right colon (Heineke-Mikulicz type closure after splitting the whole colonic segment including the cecum). The maximum pouch pressure at the pouch volume of 400 to 500ml was significantly higher (p<0.05) in the partial detubularization group (46±6.0cmH2O, mean±S. D.) than in the total detubularization group (12±1.6cmH2O). In creation of the low-pressure pouch using the right colon, detubularization should extend to the whole portion of the isolated colonic segment.
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  • Hideaki Oda, Hitoshi Hamada, Shinji Nabeshima, Masayoshi Yokoyama, Hid ...
    1990 Volume 81 Issue 4 Pages 618-625
    Published: April 20, 1990
    Released: July 23, 2010
    JOURNALS FREE ACCESS
    Immunizing mice with a transitional cell cancer (TCC) tissue in the renal pelvis, we produced a monoclonal antibody (EH14) against new epithelial antigens. After the mice were immunized repeatedly, their splenic cells were harvested and fused with NS/1 myeloma cells. The normal kidney tissue of the same patient was used on Dot blots to select the hybridoma. A a result, one hybridoma whose antibody (EH14) reacted very strongly with TCC but only faintly with normal kidney tissue or normal bladder mucosa was obtained. On immunohistochemistry, EH14 stained all of the 29 TCC tissues. EH14 also stained uterus cancer (7/7) and gastric cancer (6/6) as well as the normal squamous cell and many types of the normal epithelium. All of the lymphnodes containing metastatic bladder cancer were strongly stained with EH14. EH14, however, did not stain interstitial tissues, muscles and sarcomas. The molecular weight of the antigen recognized by EH14 was 14KD and 28KD on Western blot analysis, and the antigen was stable with formalin or ethanol. The antigen was not the same as that reported previously, and may be useful as a histological marker of TCC.
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  • Hironobu Watanabe, Masaaki Morioka, Yoshiteru Kohama, Fumito Komatsu, ...
    1990 Volume 81 Issue 4 Pages 626-629
    Published: April 20, 1990
    Released: July 23, 2010
    JOURNALS FREE ACCESS
    The first case was a 63-year-old man with edema in the lower extremities, and the second case was a 78-year-old man complaining of general fatigue and loss of apptite. Both cases had bilateral hydronephrosis and renal failure due to ureteric obstruction. Both patients with histologically proven idiopathic retroperitoneal fibrosis were studied by magnetic resonance imaging (MRI). MRI was performed using a superconducting magnet operating at 0.5 tesla and spin echo images were obtained. The retroperitoneal fibrous plaque appeared at an intermediate intensity on T1 weighted image and at a high intensity on T2 weighted image.
    They underwent intraperitoneal ureteral transposition and postoperative administration of predonisolone. Although their renal functions and hydronephroses improved after the treatments, the fibrous plaque was still observed on MRI.
    It was considered that MRI was an excellent method of choice in the diagnosis and follow-up of retroperitoneal fibrosis.
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  • Makoto Harima, Ryoji Yasumoto, Hideki Kawashima, Masazumi Asakawa, Tak ...
    1990 Volume 81 Issue 4 Pages 630-633
    Published: April 20, 1990
    Released: July 23, 2010
    JOURNALS FREE ACCESS
    An asynchronous case of triple primary malignant tumors of bladder, stomach and lung was reported.
    The patient was treated by total cystectomy and ileal conduit plasty for a bladder tumor in 1978 and subtotal gastrectomy and Billroth-I plasty for a gastric cancer in 1984.
    In 1987, a lung cancer was found and the biopsy revealed a small cell carcinoma. Though combined chemotherapy was done, he died from the lung cancer 2 years later.
    Fulfilling the Warren and Gate's criteria, this case may be said to be an asynchronous case of triple primary cancers.
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