The methods of Yarbro & Simpson and Hodgkinson & Williams were compared for the measurement of oxalic acid in urine. The mean values of coefficient of variation were 18.7% and 4.4%, respectively. With the method of Hodgkinson & Williams, the recovery of oxalic acid from urine was 87.5±13.2%.
One hundred and eight determinations of twenty-four-hour samples of urine were made using the methods of Yarbro & Simpson and Hodgkinson & Williams. The mean values of anhydrous oxalic acid per 1.73m
2 measured by these two methods were 47.1mg and 38.8mg, respectively. Values about 20% higher were obtained by the former procedure than by the latter. The correlation coefficient of the values measured by these two technique was 0.61, being far from a linear relationship.
When urine samples were collected without hydrochloric acid the values of oxalic acid measured were lower than when they were collected in bottles containing 10ml of hydrochloric acid. This difference was probably due to the fact that the acidification of urine by hydrochloric acid prevents calcium oxalate from the precipitation. So urine samples were collected in bottles containing 10ml of hydrochloric acid, and after the accumulation of a few hundred ml of urine, 1ml of hibitane was added.
Urine specimens thus obtained were not changed remarkably as to the concentrations of oxalic acid for six months at 4°C.
It was concluded that at present Hodgkinson & Williams's method was the most suitable one for the routine clinical use among the methods so far reported.
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