By the use of the intestinal perfusion technique, the secretion of nitrogen into the cecal lumen and small intestine was investigated for single comb White Leghorn cockerels of various growth stages and those fasted or fed on different diets. Nitrogen concentration in blood of those chikens was also estimated. The results are summarized as follows:
1. The cecal and intestinal secretions of total nitrogen, uric acid and ammonia were large at the early stage of growth of chickens and decreased as the growth stage proceeded.
2. The secretion of nitrogen into cecal lumen was larger in the fasting than that in the feeding on the conventional diet. When the chickens were fed on fish meal diet, the secretion of nitrogen into the cecal lumen was larger than that in the fasting. On the other hand, the secretion of nitrogen into small intestinal lumen was not affected by the change of feeding condition.
3. When the chickens were fed on the semi-purified diet containing various levels of casein, the nitrogen secretion into the cecal lumen was larger after feeding on the casein-free diet than that after feeding on 10% casein diet. The secretion of nitrogen into the cecal lumen was increased with an increase of dietary casein level.
4. The nitrogen secreted into the cecal lumen was mainly composed of non-protein nitrogen, exceeding 96% of total nitrogen, but that secreted into the small intestinal lumen contained 20-30% protein nitrogen.
5. When the large amount of nitrogen was secreted into the cecal lumen, there was high concentration of nitrogen constituents in the blood.
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