The first limiting amino acid in two samples of yeast grown on methanol, a sample of yeast grown on ethanol, two samples of bacteria grown on methanol, and two samples of brewer's yeast as a kind of reference was determined, and gross protein value of these samples with supplementation of the first limiting amino acid was assayed biologically with starting chicks.
Amino acid content of experimental diets containing 20% of crude protein, of which 12% was derived from one of the samples, was calculated from amino acid composition of the samples determined chemically. Comparing with requirements of essential amino acids for starting chicks, those deficient in the diets were picked up, and supplemented to the diets. Growth response of chicks fed the diets revealed that the first limiting amino acid in all of the samples except Yeast K grown on methanol is sulfur-containing amino acids.
Gross protein value of all the samples except Yeast K was improved significantly by supplementation of DL-methionine at a level of 1-2% of crude protein in the sample. Except a sample of brewer's yeast, Yeast K without supplemental DL-methionine and other samples with DL-methionine had excellent gross protein value over 80.

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A second collaborative experiment of seven prefectural experiment stations was carried out to investigate the effects of restricted feeding during the rearing period and of induced molting during the laying period on prolonged egg production of hens. Of the chicks used in this experiment were hatched some in spring and some in autumn.
The seven participating experiment stations were divided into two groups. The first group was composed of Tottori, Shimane, Hiroshima and Kagoshima experiment stations where hatching was timed to take place in spring. The second group consisted of Yamaguchi, Ehime and Kumamoto experiment stations where hatching took place in autumn.
In each experiment station, the chicks were divided into two groups upon becoming 4 weeks old. From thereon up to the time they reached 18 weeks of age, they were subjected to a feeding regimen where by the control group was fed ad libitum, while the other was given feed the amount of which was 60% that of the control group. At 20 weeks of age, both groups were further divided into 4 sub-groups. In both groups, two of the sub-groups were forced to molt at 80 or 92 weeks of age. The other two were forced to molt twice, the first was at 56 or 68 weeks and the second was at 96 weeks of age. They were kept until 128 weeks of age, and production and economical traits were observed.
Restricted feeding delayed the time at which the first egg was laid. On the other hand, restricted feeding relatively reduced body weight but significantly increased egg production rate from 20 to 128 weeks of age. The hens hatched in autumn gave better performance when they were force-molted at a younger age than when they were force-molted at an older age. Feed consumption per kg of egg production was less in the restricted feeding group than in the full-feeding group.

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Eight hundred and fifteen, and 692 strains of Escherichia coli were isolated from one flock of growing broiler when they were one day old (designated as A group) and 28 days old (B group), respectively.
These strains were examined for sensitivity to tetracycline (TC), chloramphenicol (CP), streptomycin (SM), sulfonamides (SA), kanamycin (KM), aminobenzylpenicilline (AB-PC) and nalidixic acid (NA).
Of 815 strains (A group) and 692 strains (B group) examined, 789 (96.8%) and 678 (98.0%) were resistant to one or more of the drugs tested, respectively.
The strains showing double and triple resistance patterns were isolated mainly from A group, however, high incidence of triple or furthermore resistance patterns were recognized among the strains isolated from B group.
In the both groups, about 10% of the strains examined were resistant to only one drugs tested, but the remaining 90% them were multiply resistant to two or more of the drugs.
The drug resistance patterns recognized commonly among strains isolated from either groups of A or B, were single (SA or TC), double (TC-SA) and triple (TC-SM-SA) resistance patterns.
Of 789 (A group) and 678 (B group) resistant strains, 134 and 223 strains were selected for R plasmids transfer tests. R plasmids were detected among the resistant strains of each group, at the almost identical rate of 40.0% (A group) and 38.6% (B group), respectively.
It was suggested that kind of drugs used, doses and frequency of medications were related to developing of resistant strains.
It was recognized that the drug resistant trains of E. coli were isolated even from day-old chicks (prefeeding) at a high frequency, and a tendency to increasing of isolation of strains having more wide multiple resistant patterns were observed in aged chickens.

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Twenty-eight hens which laid a yolkless dwarf egg are obtained from a flock of 17, 990 White Leghorn hens (1 1/2 years of age) throughout the experimental period of one month. Autopsy examinations on 20 hens revealed that the internal ovulation had taken place on the preceding day of the production of the dwarf egg. From the laying record on the remaining 8 hens following the production of the dwarf egg, it was found that the interval between the lay of dwarf egg and the succeeding normal egg was within a normal range.
The insertion of artificial yolk (AY) into the oviduct near the time of natural or induced ovulation resulted in the production of well calcified dwarf eggs similar to those naturally produced. However, the calcified dwarf eggs were not produced when the AY was inserted soon after the lay of the terminal egg of a clutch.
It is suggested that the production of yolkless dwarf eggs with shell seems to be associated with the incidence of ovulation.

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Nutritive values of Moina macrocopa, a kind of zoo plankton in fresh water, and residue of poultry manure, in diluted slurry of which microbes for Moina macrocopa were grown, were determined biologically with 369 8-day-old White Leghorn male chicks.
A fresh sample of Moina macrocopa contained 95% of moisture. An air-dried sample contained 57% of crude protein, of which gross protein value was 97 with supplementation of DL-methionine at a level of 2% of crude protein. Available energy of the sample was 3.07kcal/g. Availability of phosphorus was 45.
Available energy and gross protein value of an air-dried sample of the residue of poultry manure, which contained 25% of crude fibre, were low, being 1.31kcal/g and 40, respectively. The sample contained 4% of calcium and 2% of phosphorus, of which availability was 107.
A system of recycling feed resources with zoo plankton as one of key roles was proposed.

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[in Japanese]

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[in Japanese]

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