Japanese poultry science
Print ISSN : 0029-0254
Volume 36, Issue 6
Displaying 1-8 of 8 articles from this issue
  • Tomohiro SASANAMI, Jianzhi PAN, Makoto MORI
    1999 Volume 36 Issue 6 Pages 343-353
    Published: November 25, 1999
    Released on J-STAGE: November 12, 2008
    JOURNAL FREE ACCESS
    In quail oocytes, homologous protein to mammalian ZPC is a 33-kDa glycoprotein, which is one of the components of the inner perivitelline membrane. In order to investigate the effects of various hormones on production of quail ZPC, granulosa cells were cultured with or without FSH, LH, epidermal growth factor, insulin-like growth factor I, insulin-like growth factor II or transforming growth factor α in the presence of [3H] leucine and the incorporation of the radioactivity into ZPC was measured by immunoprecipitation of proteins. When the granulosa cells were cultured with FSH, the incorporation of radioactivity was increased. The effect of FSH was more pronounced in the cells obtained from the immature follicles than the matured one. These results demonstrate that FSH is one of the factors that stimulate ZPC production in cultured granulosa cells of Japanese quail.
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  • Yasuhiro KONDO, Chikara MATSUDA, Asaki ABE
    1999 Volume 36 Issue 6 Pages 354-363
    Published: November 25, 1999
    Released on J-STAGE: November 12, 2008
    JOURNAL FREE ACCESS
    Effects of administration of two antigens, sheep red blood cell (SRBC) and Brucell abortus (BA), via the bursa of Fabricius (BF-sensitization with antigens) on immune responses were studied in growing chicks. The effects of BF-sensitization with a bacterial antigen (BA) on IgA-producing cell count in the lamina propria of the jejunum were also examined. Antibody titers were significantly increased by BF-sensitization from 1 weeks of age to 11 weeks of age. Maximal augmentations of anti-SRBC and -BA antibody productions were observed in chicks BF-sensitized at 6 to 8 weeks of age. Responses after 4 and 7 days of intravenous booster injection of SRBC in chicks received the BF-sensitization at 1 and 6 weeks of age were higher than those of control chicks which received the i.v. administration alone. The responses were also higher than the control in chicks received the BF-administration of BA at 1, 6 and 11 weeks. Augmentation of antibody productions after the i.v. booster injection were maximal in chicks received the administration via the BF at 6 weeks of age, except for the response at 4 days after the i.v. injection of BA. Number of anti-SRBC-antibody-producing cells were increased by BF-sensitization from 1 week of age to 15 weeks of age, with maximal augmentation in chicks sensitized at 6 weeks of age. The counts after booster i.v. injection were also increased in chicks BF-sensitized from 1 week of age to 15 weeks of age. The counts received the i.v. booster injection were also maximal in chicks BF-sensitized at 6 weeks of age. Numbers of IgA-producing cells in the lamina propria of the jejunum were significantly increased by BF-sensitization with BA at 9 weeks of age as compared with those of age-matched untreated chicks. These results suggested that follicle-associated epithelium (FAE) of the BF contributed to establishment of the intestinal defence mechanism by IgA-producing lymphocytes as estimated in M cells of ruminants.
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  • Jianzhi PAN, Tomohiro SASANAMI, Makoto MORI
    1999 Volume 36 Issue 6 Pages 364-370
    Published: November 25, 1999
    Released on J-STAGE: November 12, 2008
    JOURNAL FREE ACCESS
    The extracellular investment of oocytes in a number of species contains specific adhering molecule for spermatozoa ("sperm receptor") to which spermatozoa bind as a prelude to fertilization. However, little is known about the nature of sperm receptors in avian species. In quail, the oocyte at the time of fertilization is bounded by the perivitelline membrane, which is a three dimensional network of fibers consisted of two glycoproteins with molecular masses of 33kDa and 175kDa. In order to elucidate the sperm receptor in the perivitelline membrane of quail oocyte, we examined the effects of antiserum against 33kDa protein, homologous protein of mouse sperm receptor, ZPC, on the interaction of spermatozoa to the perivitelline membrane.
    Ejaculated semen was obtained from male quail during natural mating with female. A piece of the perivitelline membrane of the largest follicle was incubated for 40min at 39C with 2×106 spermatozoa/ml with or without 100μg/ml soybean trypsin inhibitor. The membrane was stained with Schiff's reagent for counting the number of perforation. Adherent spermatozoa were stained with 4', 6-diamidino-2-phenylindole and counted under a fluorescent microscopy. Sperm-induced perfora-tion was observed by the incubation of the perivitelline membrane with spermatozoa, and the binding of spermatozoa was observed by the incubation in the presence of trypsin inhibitor. Sperm binding, as well as perforation of the perivitelline membrane by spermatozoa, was inhibited by the addition of the antiserum against quail ZPC. These studies suggest the importance of quail ZPC for the sperm adhesion to the perivitelline membrane during fertilization.
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  • Koichi SHIMAZAWA, Tsutomu ARAKI
    1999 Volume 36 Issue 6 Pages 371-376
    Published: November 25, 1999
    Released on J-STAGE: November 12, 2008
    JOURNAL FREE ACCESS
    The effects of rearing types (floor rearing and flock cage rearing) on meat color and muscle fiber characteristics in local meat-type chicken and broiler were examined. In broiler, body weight, muscle (M biceps femoris) weight and meat color were not significantly different between floor rearing and flock cage rearing. But local meattype chicken reared in flock cage had smaller body weight and muscle weight and lower lightness (L* value) of meat color than local meat-type chicken reared on floor. Both breeds (broiler and local meat-type chicken) reared on floor tend to be higher percentages area of type II A fiber than those reared in flock cage. Local meat-type chicken had a stronger tendency to be affected on distribution of muscle fiber area by rearing types than broiler. In broiler, fiber diameter was not significantly different between floor rearing and flock cage rearing. But local meat-type chicken reared in flock cage had smaller fiber diameter than local meat-type chicken reared on floor. These results suggested that the effects of rearing types on meat-color and muscle fiber characteristics in local meat-type chicken were stronger than those in broiler.
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  • Takashi BUNGO, Shin-ichi KAWAKAMI, Atsushi OHGUSHI, Masataka SHIMOJO, ...
    1999 Volume 36 Issue 6 Pages 377-381
    Published: November 25, 1999
    Released on J-STAGE: November 12, 2008
    JOURNAL FREE ACCESS
    It is known that central administration of glucagon-like peptide-1 (GLP-1) (7-36) amide suppresses feeding in rats and chicks, but the mechanisms for this response are still open with special reference to the chick. The present study was done to determine whether GLP-1 induces sleep-like behavior on the neonatal chick. Intracere-broventricular injection of GLP-1 (7-36) amide altered, in a dose-dependent fashion, the behavioral postures of chicks and induced sleep-like behavior compared with the saline control. These results indicate that GLP-1 in the central nervous system may be one of the factors to induce sleeping of the neonatal chick.
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  • Naoyuki HIRABAYASHI, Yasuhiko MATSUKI, Emako SUZUKI, Makoto USAMI, Yas ...
    1999 Volume 36 Issue 6 Pages 382-387
    Published: November 25, 1999
    Released on J-STAGE: November 12, 2008
    JOURNAL FREE ACCESS
  • H.S. KANG, J.H. SON, Y. KARASAWA, H.Y. JEON, K.H. NAHM
    1999 Volume 36 Issue 6 Pages 388-393
    Published: November 25, 1999
    Released on J-STAGE: November 12, 2008
    JOURNAL FREE ACCESS
    The study was carried out to clarify the role of cecum in protein nutrition of the chicken. It was examined in this experiment whether the blocking of entry into the ceca of urine from the cloaca and of digesta escaped from the absorption at the upper intestine by cecectomy. The effects of cecectomy on nitrogen utilization and nitrogen excretion were compared between chickens fed a 5% protein diet and a 5% protein diet supplied with urea. When a 5% protein diet was fed, total nitrogen excretion and nitrogen balance were significantly decreased and increased by the cecectomy, respectively. Similar responses were obtained in chickens fed on a 5% protein diet supplied with urea. The cecectomy in both dietary groups significantly decreased uric acid excretion (P<0.05), urea and ammonia excretion tended to increase and decrease, respectively. None of blood uric acid, urea and ammonia concentrations were significantly changed by cecectomy in either dietary group. It is concluded that the cecectomy decreased uric acid production and tended to increase nitrogen balance, in similar manners that cecal ligated chickens fed on a low protein diet and a low protein diet plus urea.
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  • 1999 Volume 36 Issue 6 Pages 394-397
    Published: November 25, 1999
    Released on J-STAGE: November 12, 2008
    JOURNAL FREE ACCESS
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