The Japanese journal of animal reproduction
Print ISSN : 0453-0551
Volume 10, Issue 4
Displaying 1-8 of 8 articles from this issue
  • A. HANADA, K. HIROE, T. TOMIZUKA
    1965 Volume 10 Issue 4 Pages 109-113
    Published: 1965
    Released on J-STAGE: May 15, 2008
    JOURNAL FREE ACCESS
    1. Microspectrophotometric observations have been made of the DNA content of bull spermatozoa during storage in yolk-citrate diluent at 4°C to reexamine the earlier results by SALISBURY et al1). 2. It was found that hydrolysis for twelve minutes was improper to sperm DNA. The optimum hydrolysis time with IN-HCI at 60°C was about 7 minutes. 3. There was a significant difference in the head area of spermatozoa between live and dead cells, judged by Eosin staining technique. In dead spermatozoa, the increase of the head area was recognized. 4. The amount of Feulgen-DNA in bull spermatozoa decreased significantly after 5th day of the storage when examined at 0, 5th, 10th and 19th days.
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  • M. IMAI, S. SASAMOTO, T. SUZUKI
    1965 Volume 10 Issue 4 Pages 114-119
    Published: 1965
    Released on J-STAGE: May 15, 2008
    JOURNAL FREE ACCESS
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  • IV. Effect of unfractionated sheep pituitary gonadotrophin (Vetrophin) on the spermatogenic function in bull
    A. OGASA, Y. SUGAWA, S. MATSUYAMA
    1965 Volume 10 Issue 4 Pages 120-123
    Published: 1965
    Released on J-STAGE: May 15, 2008
    JOURNAL FREE ACCESS
  • T. SUGIE, T. SOMA, H. ONUMA
    1965 Volume 10 Issue 4 Pages 124-127
    Published: 1965
    Released on J-STAGE: May 15, 2008
    JOURNAL FREE ACCESS
    Two Holstein heifers were superovulated by the administration of gonadotropins, PMS and HCG, to donate the fertilized ova for the ova transplantation in cattle.
    From one of the donors, a morula stage embryo was collected using non-surgical technique and three ova were recovered from another one following the slaughter.
    The morula embryo from the former donor and an 8 cell ovum from the latter, were transferred non-surgically into the uterine horn of two recipient heifers individually, using specially designed apparatus for the ova transfer in cattle. Both of the recipients conceived following the trial.
    A normal bull calf was born 268 days after transfer of the morula stage embryo. It was confirmed by the blood typing test that the calf originated from the transferred embryo. Another recipient aborted at the 80th day of pregnancy.
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  • T. NAKAHARA, Y. KANEDA, T. KATAOKA, M. YAMAUCHI
    1965 Volume 10 Issue 4 Pages 128-136
    Published: 1965
    Released on J-STAGE: May 15, 2008
    JOURNAL FREE ACCESS
    In our ealier report, it was demonstrated that the antihormone against human chorionic gonadotrophin (Anti-HCG) might be produced easily in cattle. The present studies were designed to investigate the formation of the antihormone against pregnant mare serum gonadotrophin (Anti-PMS) in cattle receiving this hormone. Results obtained were summarized as follows:
    1) Responses occurred in immature mice to PMS used in present studies.
    Gonadotrophic potencies of PMS used in present study were assayed biologically by employing immature female mice and the results obtained were shown in Fig. 1. Each samples of PMS solutions at the concentrations of 1.5 IU, 3.0 IU, 6.0 IU and 12.0 IU/ml. were injected subcutaneously into inguinal region of immature female mice twice daily for 2 days (total of 1 ml.), and these animals were killed 100 hours after the first injection. Cornification of vaginal epithelial cells, formation of corpora lutea and haemorrhagic follicles in the ovaries and ovarian weight were examined, and the relations between the dosage (IU) and the responses were shown in the table presented under Fig. 1.
    2) Method to determine bovine serum Anti-PMS.
    Determination of bovine serum Ante-PMS was performed biologically by using immature female mice. Two parts (2/3 ml.) of undiluted bovine serum sample and 1 part (1/3 ml.) of saline solution of PMS with graded concentrations were incubated for 1 hour at 37°C, and then these mixed samples were assayed by the same method as that above mentioned for examining the potencies of PMS. This method (original method) was compared with two other methods (serumdilution method and simultaneous injection method), and no appreciable difference in PMS inhibiting titers between the original method and the two other methods could be found (Table 3 and 4).
    3) Formation of Anti-PMS in cattle following injection of PMS.
    Four bull calves were injected with large quantities of PMS intravenously or intramuscutlarly. One of them (No. 1) was received single injection of 180, 000 IU of PMS, and the other 3 animals (No. 2-4) were received 13-15 injections of 1, 000 IU or 2, 000 IU of PMS (total of 1, 5000-30, 000 IU) at 3 or 7 days interval. The results of the determination of Anti-PMS in the serum samples collected from these animals were shown in Table 1. In 3 animals (Nos. 1, 2 and 3) the determinations of Anti-PMS were negative on the serum samples collected at 5 to 50 days after the PMS injection. But in the remaining one (No. 4) which was injected intramuscularly with 2, 000 IU of PMS weekly for 13 times, Anti-PMS was determined to be positive on the serum sample collected an 14 days after the last injection, the inhibiting titer of the serum being 18.0 IU per ml. or more.
    4) Formation of Anti-PMS in cattle following reinjection of PMS.
    Four animals same as those employed in the previous experiment were reinjected with PMS 92-152 days later. Two of them were reinjected daily with 2, 000 IU of PMS for 7 days and the remaining 2 were received weekly 10 reinjections of 2, 000 IU. By these reinjections, serum Anti-PMS become positive, in all of them, their inhibiting titers being about 4.5-9.0 IU per ml. as shown in Table 2. The potencies of the serum Anti-PMS increased gradually from 5 to 10 days after the last PMS injection, reached the maximum level about 10 days later, and thereafter fell rapidly.
    5) From these results, it seemed that the Anti-PMS might not be produced in cattle easily by injecting the dosage of PMS which was used generally for treating ovarian disorders.
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  • S. INOUÉ
    1965 Volume 10 Issue 4 Pages 137-138
    Published: 1965
    Released on J-STAGE: May 15, 2008
    JOURNAL FREE ACCESS
    Einige bekannte Kennzeichnungen für die Versuchsnager eignen sich besonders zu dem Falle des Goldhamsters (Mesocricetus auratus auratus) nicht. Der Verfasser, ebenso wie Jung1), verwendet aber auf die Markierung Schnitte in das Fell des Rücks nach dem Schema mittels des 7. 4. 2. 1-Kodes (Abb. 1), das im Randlochkartenfeld oft gebraucht worden ist. Dadurch kann man ein tausend Tiere von Nr. 000 bis Nr. 999 gleichzeitig unterscheiden (wenn man nur bis hundert oder zehn Tiere markieren muss, gebrauche, respektiv, die linken und rechten Längsstreifen oder den mittleren). Solche Methode kann auch zu weissen Ratten sowie Mäusen angewandt werden: diesmal darf man nur Pikrinsäure oder andere Farblosung statt des Schnitts auf das Fell auftragen. Diese Kennzeichen sind etwas besser im Vergleich mit der Teuffelischen Methode, weil die geschnittene oder aufgetragene Tupfe einfacher, weniger in der Art und also leicht zu machen und lesen sind.
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  • I. Maturation division of the oocytes in ovulated cattle
    I. ISHIBASHI
    1965 Volume 10 Issue 4 Pages 139-141
    Published: 1965
    Released on J-STAGE: May 15, 2008
    JOURNAL FREE ACCESS
  • II. Maturation division of the oocytes in superovulated cattle treated with gonadotronhin
    I. ISHIBASHI
    1965 Volume 10 Issue 4 Pages 142-147
    Published: 1965
    Released on J-STAGE: May 15, 2008
    JOURNAL FREE ACCESS
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