The effects of adrenalin, acetylcholine and oxytocin on the spontaneous motility of the excised uteri of rats have been observed under the same experimental method as written in the previous report. And in this experiment, every excised uteri were examined under the condition of the load with four and five times' weight for each uterus, because under these condition, the charac-teristic uterine motility, as the contraction of uterus and the stabilized tonus, at each stage of the estrous cycle was clearly observed.
The results obtained were as follows;
1. The spontaneous uterine motility was inhibited markedly after the administration of adre-nalin and the tonus also was descended at each stage of the estrous cycle.
2. By the acetylcholine administration, the spontaneous uterine motility was excised remark-ably and the tonus was also raised clearly at the estrus stage.
3. The excised activity due to the administration of acetylcholine was inhibited strongly by the subsequent administration of adrenalin.
4. In the case of oxytocin administration, the most strongest contraction, such as tetanic contraction of the uterus was observed at the estrus stage.
5. This strongest contraction due to the administration of oxytocin was relaxed clearly by the subsequent treatment of adrenalin.

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Fifty five bloodless uterine washings were collected from 22 normal and 25 infertile lactating Holstein-Friesian cows.
Uterine washings were obtained using an endotracheal tube with a polyethylene bac placed in the uterus per vaginam. The uterine cavity was flushed repeatedly three times with 100 ml phy-siological saline. The washings were stored in an ice box.
The recovery rate of flushing fluid used to obtain uterine washings was 50 % in average (4070 % in range).
The lipid content was estimated by the silver dichromate method of Bloor as modified by Katsura-Hatakeyama, toatal-N was determined by the micro-Kjeldahl procedure and reducing sugar by the colourimetric technique of somogyi, glycogen by the anthrone method of Good and Morris as modified by Kanemaki¹⁴⁾.
The results obtained were as follows.
1. In normal group, the levels of total lipid, total-N and reducing sugar in uterine washings showed a definite variation with stage of estrous cycle. Each amount of three constituents was lowest at estrus and increased gradually during the luteal stage. The values within 5 days to 15 18 days postestrus were maintained significantly higher than that at estrus.
The ratios of the constituent levels at 1518 days postestrus to those at estrus were 2.8 in total lipid, 3.9 in total-N and 5.1 in reducing sugar, respectively. Glycogen was detected little in the washings.
2. In the groups of acute and chronic-catarrhal endometoritis, the values of three constituents in uterine washings recovered at mid-luteal stage (10 to 15•8 days postestrus) were lower than those at the same stage of cycle in the normal group, and significant difference appeared in total lipid and total-N levels of both groups.
3. Each mean value of the components in follicular cyst and lutein cyst groups was situated between the values at estrus and mid-luteal stage of cycle. Total lipid and total-N levels in lutein cyst group were significantly lower than those at the same stage (10 days postestrus) in the normal group.
4. Levels of the three constituents in ovarian atrophy group were lowest among the five in-fertile groups, and the values of total lipid and total-N were significantly lower than those at estrus in the normal group.

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Luteinizing hormone was extracted from the pituitary of cattle foetuces, and its content was determined by the bioassay method on the rat ventral prostate weight (GREEP et al. 1942).
LH was found in the extract obtained from the foetal pituitary of both sexes from the 5th month of gestation. The content of the pituitary hormone seemed to increase with the progress of gestation.
The luteinizing hormone content was higher in the male pituitaries than in the female ones, and this difference became remarkable in the foetuses of the 89th month of gestation.

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The effect of some amides on the motility of bull spermatozoa was investigated before and after pellet freezing of semen.
Each aliquot of collected semen was diluted ten times with egg yolk-citrate diluent containing one of amides (form-, lact-, acet-, propion-, malo-, butyl-, valer-), ethylene glycol, DMSO, or glycerol (control) at a final level of 0.31, 0.93, 1.55, 2.17 and 3.1 M after dilution.
Sperm motility was estimated with television-video record equipment connected with phase-contrast microscope.
When diluted semen was incubated at 20°C for 30 min., the best sperm motility was observed in lactamide diluent as well as in glycerol diluent (Fig. 1).
Other amides were inferior to these two diluents, but acetamide maintained rather good mo-tility which was followed by propionamide, formamide and malomide. Effect of malonamide over 1.55 M level on sperm motility was unable to investigate owing to its low solubility. In butylamide sperm motility dropped near to zero at 1.55 M level and in valeramide sperm died immediately after dilution even at 0.31 M level. When diluted semen was cooled to 5°C and frozen on a dry ice block in 0.1 ml pellet form after equilibration for 36 hrs and thawed on a metal plate at 40°C, all amides, except butylamide and valeramide, showed some protective effect for sperm survi-val against freezing injury (Fig. 2).
Among them, the best protective effect was observed in lactamide diluent, then in acetamide and formamide diluent, when semen was thawed without thawing media. Propionamide and ma-lonamide gave low protective effect.
Striking and contrasted changes of post-thawed sperm motility were observed by redilution of thawed semen with thawing media (buffered milk-isotonic to raw semen)⁶⁾ in the samples diluted with formamide and lactamide diluents.
In the former, the motility was greatly improved at 0.93 M level, but in the latter the moti-lity was injured significantly by redilution.
These phenomena is inferred to be related with the permeability of the amides into the cells and also with the degree of injury of living sperm after freezing and thawing.
From these findings, formamide and lactamide will can be used as new cryoprotective agents for spermatozoa.
Fertility triale are undergoing with these amides in cattles, swine and rabbit.
Parts of the paper had been presented at the 13th annual meeting of The Japan Frozen Semen Research Society on Sept. 2 3, 1971 and at the antumn meeting of The Japanese Soc. Anim. Reprod. at Kyoto on Oct. 27, 1971.

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The ovulating hormone (O. H.) content in the mouse pituitary was determined throughout the normal 4-day estrous cycle, bioassayed by the extra-ovulation inducing potency. The preovulatory decrease of O. H. onset soon after 10 am of the day of proestrus from the maximal level in the cycle, and cotinued through 1pm, 4 pm, 6 pm, 9 pm, and finally the content of O. H. reached to the minimal level at 0 am of the day of vaginal estrus. It is revealed that the release of O. H. is continued further even after the period, 5 pm of the day of proestrus, when the enough quantity of O. H. to cause ovulation has already been released.
Since the onset of decrease of pituitary potency was observed at 10 am, the injections of pentobarbital (Nembutal) was started at 10 am of the day of proestrus for the blockade of ovulation. Although the single injection was not effective, the repetition of additional injections for every 2 hrs. until 10 pm was attained the blockade of ovulation in all animals. This is also the case where the injection is started at 1 pm. These results may indicate that pentobarbital anethesia which covers all over the term of preovulatory O. H. decrease in the pituitary, is able to delay the periodical ovulation by 24-hrs.
A sudden increase of pituitary O. H. content was observed from noon to 4 pm of the day of metestrus. By this increase the content of O. H. attained to a level of the early morning of pro-estrus. Repeated injections of Nembutal for every 2 hrs. from noon to 10 pm on the day of metestrus also caused a delay in ovulation by one day together with a delay in appearence of the vaginal smear of proestrus.
In the period from noon to 4 pm of the days of estrus and diestrus, an appreciable change in 0. H. content of the pituitary was observed.
It is noteworthy that the time of fluctuation of O. H. content in pituitary was found every day around the time coresponding to the so-called "critical period" in the day of proestrus.

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[in Japanese]

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The effects of combined use of glycerol and DMSO on the post-freeze livability of boar sper-matozoa diluted in honey-yolk diluent⁹⁾ were examined. The final glycerol or DMSO levels in diluted semen were 0, 1, 2 and 4 % (v/v) respectively. Diluted semen was frozen by pellet-form method¹⁰⁾, and preserved at -79°C for 24 hours. Frozen semen was thawed on the metal plate at 38°C, and the motility was estimated microscopically at 38°C. Eosin-nigrosin stain was made to determine the survival rate.
The motility and survival results are shown in Table 1 and 2.
The results of statistical analyses on the post-freeze motility and survival are shown in Table 3.
The best result was obtained in the sample containing 2% glycerol only. It was indicated that the addition of DMSO to the diluent containing glycerol did not improve the post-freeze livability.

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