The Japanese journal of animal reproduction
Print ISSN : 0453-0551
Volume 20, Issue 3
Displaying 1-7 of 7 articles from this issue
  • Tetsuyuki SUGA
    1974 Volume 20 Issue 3 Pages 81-82
    Published: November 30, 1974
    Released on J-STAGE: May 15, 2008
    JOURNAL FREE ACCESS
  • Masaroshi HAYASHI
    1974 Volume 20 Issue 3 Pages 89-94
    Published: November 30, 1974
    Released on J-STAGE: May 15, 2008
    JOURNAL FREE ACCESS
    The persent study was designed to clarify the composition of the blood of adult Beagle bit-ches during the estrous cycle, pregnancy, and the postpartum period. Ultramicroanalytical procedures were used to determine serum non-protein nitrogen, cholinesterase, glucose, blood urea nitrogen, cholesterol, alkaline phosphatase, total protein, albumin/globulin ratio, calcium and creatinine values, hemoglobin, hematocrit, and erythrocyte and leukocyte count of pheri-pheral blood.
    Alterations observed during the estrous cycle were an increase in cholinesterase and non-protein nitrogen values in the anoestrous stage and an increase in alkaline phosphatase value in the diestrus stage. On the other hand, the serum choesterol value was low in the early stage of anoestrus and the creatinine value also low in the diestrous and anoestrous stages.
    The serum cholinesterase value increased gradually from the 10th day of pregnancy to the day of parturition. The albumin/globulin ratio tended to decrease during gestation. A de-crease in erythrocyte count, hemoglobin, and hematocrit value occurred in the late period of pregnancy.
    The serum cholinesterase and alkaline phosphatase values increased in the postpartum period, whereas the blood urea nitrogen value decreased in the same period. No other serum components after parturition were significantly different from those during pregnaacy. All the hematological values were lower in the postpartum period than during gestation.
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  • Ikuo DOMEKI, Tatsuo NAKAHABA, Makoto YAMAUCHI, Takuo MAKING
    1974 Volume 20 Issue 3 Pages 95-100
    Published: November 30, 1974
    Released on J-STAGE: May 15, 2008
    JOURNAL FREE ACCESS
    Radioimmunoassay method (RIA) was compared to competitive protein binding assay (CPBA) and fluorometric assay method for the determination of plasma progesterone of the cow. Peri-pheral blood progesterone of the cow during estrous cycle wasdetermined by RIA.
    High potent antiserum against progesterone-3-oxime-BSA (optimal dilution, 1 : 40, 000) was prepared for RIA. Calibration curve made by the antiserum showed clear lineality between 0 and 400 pg of progesterone, and percent binding of 3H-progesterone was 70% or higher at 0 pg. The antiserum also showed highly specificity against progesterone, and no significant cross reac-tion was found against other various steroids except 5α-pregnanedione.
    In RIA method, values of progesterone determined on the crude samples which was extracted from blood samples with ether (direct method), were compared to those determined on the purified samples obtained by earring out column chromatography (chromatographic method). There was a good correspondence in the values of progesterone between direct and chro-matographic methods.
    No significant differences were foundbetween the direct method of RIA, CPBA and flu-orometric assay method, in valuesof progesterone, determined on the same plasma samples collected from the cow atvarious stages of the estrous cycle.
    Concentrations of plasma progesterone, determined by the direct method of RIA in 9 cows during estrous cycle, showed minimum level (0.2-0.3 ng/ml) at estrus and maximum level (3.2-5.8 ng/ml) during luteal stage of 11 to 16 days after ovulation. The concentration was rapidly decreased about 4 to 6 days before the next ovulation.
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  • Motoaki UMEZU, Genzaburo MIURA, Saburo TAKEUCHI
    1974 Volume 20 Issue 3 Pages 101-104
    Published: November 30, 1974
    Released on J-STAGE: May 15, 2008
    JOURNAL FREE ACCESS
    Effects of 2 synthetic LH-RH (decapeptide amide-p-Glu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2: (Gn-RH), nonapeptide-des-Gly-NH210, Proethylamide9: (Gn-RH-I) on ovulation of adult and immature rats were investigated.
    Results obtained were as follows: 1) In adults, LH-RH disolved in 0.5 mlsaline was injected i. v. at 2:00 PM of diestrus or metestrus day.
    Rats were killed on the following day after LH-RH injection on diestrus day and on 4 days after the injection of metestrus day to examine the ampulla for ova.
    i) Ovulation was observed in rats injected with more than 2 μg of Gn-RH or more than 0.2 μg of Gn-RH-I on diestrus day.
    ii) When 50 μg of Gn-RH or more than 2 μg of Gn-RH-I was injected, ovulation was delayed 1 day.
    2) In immature rats aged 2427 days, 3 IU of PMS was injected s. c. at 9:3010:00 AM to induce ovulation. Then clomiphene (Clomiphene-citrate: 5 mg per 100 g B. W. in 30% ethanol, after 6 hrs of PMS treatment) or Nembutal (4 mg per 100 g B. W., after 49 hrs of PMS treat-ment) was injected to block ovulation.
    i) In clomiphene treated rats, ovulation was induced by more than 0.2 μg of Gn-RH or Gn-RH-I.
    ii) In Nembutal treated rats, ovulation was induced by more than 2 pg of Gn-RH or more than 0.2 μg of Gn-RH-I.
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  • Toshihiko NAKAO, Kunitada SATO, Hitoshi ONO, Masaru MIYAKE
    1974 Volume 20 Issue 3 Pages 105-110
    Published: November 30, 1974
    Released on J-STAGE: May 15, 2008
    JOURNAL FREE ACCESS
    Progesterone, estrone, estradiol 17-β and estriol in the peripheral blood serum of 81 cows with cystic ovaries of which 23 exhibited continuons estrus, 28 showed irregular estrus and 30 were anestrus, were estimated by radioimmunoassay, and their relation to the sexual behavior and the effect of intramuscular injection of 20 mg of betamethasone (Betsolan, GLAXO) were discussed.
    (1) The mean levels (±S. D.) of serum progesterone, estrone, estradiol 17-β, estriol were as follows; 0.10 ± 0.006 ng/ml, 37.8± 4.9 pg/ml, 125.3 ± 3.4 pg/ml, 23.6 ± 3.2 pg/ml, in continuous estrous cows; 0.31 ± 0.008 ng/ml, 4.9 ± 2.3 pg/ml, 48.5 ± 3.0 pg/ml, 4.5 ± 6.1 pg/ml in irregular estrous cows and 0.46 ± 0.056 ng/ml, 10.7 ± 4.5 pg/ml, 17.4 ± 5.8 pg/ml, 3.6 ± 5.2 pg/ml in anestrous cows, respectively.
    (2) Cows having higher concentrations of progesterone showed higher conception rates (pregnant cows/treated cows) and conceived in shorter periods following the treatment as compared with those having lower concentrations of it. On the other hand, cows having higher concentrations of estradiol 17-β required longer days to conceive than those having lower concentrations of it, although there were no differences in their conception rates.
    (3) Conception rates and their confidential limits (P ?? O.05) and the average days (± S. D.) from treatment to conception were 78.3%, 89.758.9%, 77 ± 54 days for continuous estrus cows, 67.7%, 78.548.8%, 93 ± 61 days for irregular estrous cows and 83.3%, 92.368.5%, 58± 31 days for anestrous cows, respectively.
    (4) From these findings, it may be concluded that there is a correlation between sexual behavior and the blood levels of estrogens and progesterone of cows with cystic ovaries, which also relate to the effect of treatments with corticosteroids.
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  • Seiji TAKAYAMA, Mikio FUKAZAWA
    1974 Volume 20 Issue 3 Pages 111-114
    Published: November 30, 1974
    Released on J-STAGE: May 15, 2008
    JOURNAL FREE ACCESS
  • Yasunori WADA, Masao SINO
    1974 Volume 20 Issue 3 Pages 115-121
    Published: November 30, 1974
    Released on J-STAGE: May 15, 2008
    JOURNAL FREE ACCESS
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