Journal of Environmental Conservation Engineering
Online ISSN : 1882-8590
Print ISSN : 0388-9459
ISSN-L : 0388-9459
Volume 36 , Issue 7
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  • Xiaoyu HUANG, Akiko MATSUMOTO, Akihiro OHNISHI, Masaru SAKAMOTO, Naosh ...
    2007 Volume 36 Issue 7 Pages 501-508
    Published: July 20, 2007
    Released: December 25, 2008
    JOURNALS FREE ACCESS
    The present study was aimed at enrichment of hydrogen fermentative microflora that can utilize garbage as fermentation substrate. It was shown that stable hydrogen fermentation was performed using enriched microbes from sewage sludge compost. During the enrichment culture, the microflora were analyzed by the FISH method and the PCR-DGGE method. As a result, predominant microbes of hydrogen production were determined to be from the genus Clostridium belonging to a Gram positive Low G+C group. Furthermore, it was supposed that genus Bacillus contributed to the stability of hydrogen productivity from garbage by genus Clostridium. In the batch culture, under pH control at 6.0, it was ascertained that enriched microflora obtained from sewage sludge compost had sufficient hydrogen productivity using garbage, and yielded 2.03mol-H2/mol-hexose. It is supposed that the microflora of sewage sludge compost is effective as inoculum of the hydrogen fermentation system when using garbage as substrate.
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