Journal of Environmental Conservation Engineering
Online ISSN : 1882-8590
Print ISSN : 0388-9459
ISSN-L : 0388-9459
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Volume 39, Issue 7
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  • Analysis of the Degradation Characteristics and Microbial Community during Anaerobic Digestion of Methanol Wastewater
    Feng YAN, Takuro KOBAYASHI, Yu-You LI, Tatsuo OMURA
    2010 Volume 39 Issue 7 Pages 426-433
    Published: July 20, 2010
    Released on J-STAGE: October 11, 2011
    DOIhttps://doi.org/10.5956/jriet.39.426
    JOURNAL FREE ACCESS
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    The effect of hydraulic retention time (HRT) on the anaerobic digestion of wastewater containing methanol was investigated in a continuous experiment using three CSTR digesters. Three digesters continuously fed by artificial wastewater containing methanol were operated at a HRT 2,5 and 15 days, respectively. No accumulation of volatile fatty acids were observed in each reactor during the operation. The removal efficiency of COD ranged from 90% to 92% . Methane-producing activity tests suggest that direct methanol-utilizing pathway is predominant in the methane fermentation of the methanol. The results of cloning analysis revealed that sarcina-shaped cells beloning Archaea were predominant in the two reactors with HRT 2 days and HRT 15 days. FISH analysis results suggest that Methanosarcina-targeted cells were predominant in the reactors with HRT 2 days and HRT 15 days.
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