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Volume 23 (1982)
- Issue 4 Pages 411-
- Issue 3 Pages 261-
- Issue 2 Pages 129-
- Issue 1 Pages 88-
Volume 23, Issue 1
Displaying 1-4 of 4 articles from this issue
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In Vitro Life Span and UV Sensitivity in Cells Obtained from a Multimalf ormed Premature Human FetusSADAYUKI BAN, SHOZO IIDA, NOKIN CHIN, AKIO A. AWA1982 Volume 23 Issue 1 Pages 88-98
Published: March 15, 1982
Released on J-STAGE: June 16, 2006
JOURNAL FREE ACCESSCultured human cells were established from various organs of a congenitally abnormal fetus. The life span of lung cells was equivalent to 62 PDN, but those of cells from skin, liver, bone marrow and kidney were very short. Chromosome analysis of cells derived from the lung and skin showed few aberrations, but aneuploid cells and chromosomal aberrations were frequent in the kidney cells. There was no difference in UV sensitivity between lung cells and kidney cells; both were about twice as sensitive as normal human fibroblasts. In this study, we did not find a correlation between the UV sensitivity and the length of life span in vitro, the amount of chromosomal aberrations or tissue origin of the cells.View full abstractDownload PDF (642K) -
T. UEDA, Y. SUZUKI, R. NAKAMURA, M. NAKAHARA, C. SHIMIZU1982 Volume 23 Issue 1 Pages 99-104
Published: March 15, 1982
Released on J-STAGE: June 16, 2006
JOURNAL FREE ACCESSThe appearance of radioactive Co in the liver of abalone from sea water was examined to consider the effect of chemical forms of Co(CoCl2 and cyanocobalamin; vitamin B12) in sea water upon the metabolisms in marine organisms. Organic 57Co(cyanocobalamin) from sea water appeared in the liver of abalone combining with a constituent with a molecular weight of 4 × 104. The constituent had the activity of vitamin B12, while inorganic 60Co(CoCl2) appeared combining with three constituents with molecular weights more than or equal to 1.5 × 106, 7 × 103 and less than or equal to 1.5 × 103 which did not show the activities of vitamin B12. The effect of chemical forms of Co in sea water is significant in its accumulation by some species of marine organisms.View full abstractDownload PDF (243K) -
TAKU KOYANAGI, MOTOKAZU NAKAHARA, MITSUE MATSUBA, SHIGEKI HIRANO1982 Volume 23 Issue 1 Pages 105-118
Published: March 15, 1982
Released on J-STAGE: June 16, 2006
JOURNAL FREE ACCESSRetention, distribution and excretion of radionuclides in a cephalopod, lidako, Octopus ocellatus, were observed by administering radioisotopes (54Mn-, 59Fe-, 60Co-, 65Zn-chlorides and 57Co-cyanocobalamin) into the mantle cavity by injection. Whole body radioactivity of the octopus was measured periodically after the injection for one to ten weeks to obtain the retention curve. At different stages after the injection, the sacrificed octopus was dissected into eight parts to examine the distribution of radionuclides and its change with the lapse of time. For some organs of the octopus, gel filtration chromatography (GFC) with Sephadex G-75 was applied to elucidate the binding of radionuclides with the constituents of the octopus.
Excretion patterns consisted of two or three components for every nuclides except 57Co-cyanocobalamin which showed monophasic elimination. 54Mn was lost most rapidly whereas the longest biological half-life was shown by 59Fe. The most significant distribution of radioactivity was observed for 57, 60Co and 59Fe in the branchial heart of the octopus, while no specific accumulation of 54Mn and 65Zn was shown in this organ. The different accumulation mechanisms between each chemical form of cobalt and among the nuclides were suggested from the GFC elution profiles of radioactivity in the branchial heart and the liver.View full abstractDownload PDF (466K) -
SHOZO SUZUKI1982 Volume 23 Issue 1 Pages 119-127
Published: March 15, 1982
Released on J-STAGE: June 16, 2006
JOURNAL FREE ACCESSErythrocytes irradiated with 60Co γ-rays took up more extracellular 42K+ and released more intracellular 42K+ during and immediately after irradiation at 37°C than did unirradiated control. The result of K+ transport was contrasted with the well-known K+ loss on prolonged incubation. However, total K+ and ATP levels in irradiated cells did not change significantly under the same conditions. Such radiation-induced K+ exchange was insensitive to 1 mM ouabain, an inhibitor of active transport, but sensitive to 20 mM NaF, an inhibitor of glycolysis. These results suggest that this K+ exchange is associated with glycolysis, but not with ouabainsensitive ATPase.View full abstractDownload PDF (288K)
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