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Volume 27 (1986)
- Issue 4 Pages 325-
- Issue 3 Pages 255-
- Issue 2 Pages 141-
- Issue 1 Pages 99-
Volume 27, Issue 1
Displaying 1-5 of 5 articles from this issue
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KANG WEI, SATORU TOMITA, ETSUKO YANAGAWA, KENSHI SUZUKI1986 Volume 27 Issue 1 Pages 99-104
Published: March 15, 1986
Released on J-STAGE: June 16, 2006
JOURNAL FREE ACCESSThe pBR322 DNA was irradiated with black light in the presence of 8-methoxypsoralen and digested by various restriction endonucleases including Hin dIII and Rsa I. It was found that for every restriction enzymes examined digestion products specific for pretreated DNA appeared as expected from the inhibition of cleavage presumably due to the formation of corsslinks at the cutting site or within the recognition sequence. Cleavage by Hin dill was affected sensitively by pretreatment of DNA with irradiation in the presence of 8-MOP. The results were discussed in terms of stringency of enzymes to the structural changes of DNA and/or of the unique base sequence favorable to form crosslink.View full abstractDownload PDF (173K) -
HIROSHI SATO, YOSHIHISA KUBOTA, SENTARO TAKAHASHI, OSAMU MATSUOKA1986 Volume 27 Issue 1 Pages 105-111
Published: March 15, 1986
Released on J-STAGE: June 16, 2006
JOURNAL FREE ACCESSEffect of calcium diethylenetriaminepentaacetate (Ca-DTPA) and macrophage activating substances, lipopolysaccharide (LPS) and zymosan, on 59Fe release from rabbit alveolar macrophages which ingested 59Fe-iron dextran was studied in vitro. The alveolar macrophages harvested by lung lavage, loaded with the 59Fe-iron dextran were cultured in Hanks'' balanced salt solution (HBSS) at 37°C for 8 hrs with or without the agents. With the addition of 10 mM Ca-DTPA, the 59Fe release was increased to about 4-fold of the control level. Effect of LPS or zymosan was not as large as that of Ca-DTPA, but the simultaneous use of LPS or zymosan with Ca-DTPA enhanced the more elevated 59Fe release.View full abstractDownload PDF (319K) -
YOSH MARUYAMA, JOSE M. FEOLA, CLARA MAGURA, J. LAWRENCE BEACH1986 Volume 27 Issue 1 Pages 112-120
Published: March 15, 1986
Released on J-STAGE: June 16, 2006
JOURNAL FREE ACCESSR.B.E. of Cf-252 on lymphoid tissue was assessed by radiation study of spleen weight loss following acute Co-60, and low dose rate (L.D.R.) Cs-137 and Cf-252 irradiations. Acute Co-60 and L.D.R. Cs-137 dose-response followed two component exponential curves with a 1.3-fold greater effect of L.D.R. Cs-137 vs. acute Co-60 on the first slope and 1.9-fold greater effect for the 2nd slope. L.D.R. Cf-252 response was 1.3x greater than acute Co-60 but was 1.0 vs. L.D.R. Cs-137 for the first slope indicating a similar effect of Cf-252 mixed neutron/gamma radiation to L.D.R. gamma radiation in producing spleen shrinkage. There was no effect of different sequences and schedules of mixing acute Co-60 with Cf-252 irradiation observed by endogenous CFU-S survival. The R.B.E. of 1.0-1.9 indicates that lymphohemopoietic in vivo, presumably well oxygenated, does not respond acutely or as sensitively as hypoxic tumor where R.B.E. is 5-7.View full abstractDownload PDF (386K) -
KUNIO SHIRAISHI, HISAO KAWAMURA, GI-ICHIRO TANAKA1986 Volume 27 Issue 1 Pages 121-129
Published: March 15, 1986
Released on J-STAGE: June 16, 2006
JOURNAL FREE ACCESSTotal diet samples were collected from two urban areas in Japan, Sapporo and Kyoto, during the period from 1967 to 1968. The samples, ashed by dry-ashing and acid-digestion, were analyzed for elements by atomic absorption spectroscopy with the flame and electrothermal atomization. Average daily intake found in the two cities was determined as follows: sodium 4.4 g, potassium 1.6 g, magnesium 0.19 g, iron 11 mg, zinc 7.2 mg, aluminium 4.4 mg, manganese 3.8 mg, rubidium 1.9 mg, copper 1.3 mg, nickel 0.25 mg and molybdenum 0.16 mg, per person per day. By comparison with the ICRP Reference Man data, a little higher values were observed for sodium, strontium and manganese. For some other elements e.g. calcium, nickel, molybdenum and copper, about half the level of corresponding daily intake selected by ICRP for the Western Europeans and North Americans was noticed.View full abstractDownload PDF (415K) -
OSAMU YAMAMOTO, IZUMI FUJI1986 Volume 27 Issue 1 Pages 130-139
Published: March 15, 1986
Released on J-STAGE: June 16, 2006
JOURNAL FREE ACCESSAqueous adenine solutions of 5 × 10-4 M (containing 14C-adenine and buffered pH 7.0) were irradiated with 60Co gamma-rays and 3H beta-rays from tritiated water in the presence of N2, O2, N2O or t-BuOH-N2. Thin-layer chromatography (TLC) was carried out bidimensionally for separation of the radiolytically produced products and autoradiography was performed. Considerable differences were observed in the doseyield curves for the decomposition of adenine and for the product formation between gamma- and beta-radiolyses. As for the degradation yield, oxygen enhancement ratios, 3.19 in gamma-irradiation and 1.08 in beta-irradiation, were obtained at a dose of 3.0 × 103 Gy. Similar products were produced both under N2 and O2, but there were found a specific reaction of t-butanol radical with adenine, the high yield of hypoxanthine under N2O, and the higher degradation of the TLC origin-fixed products in beta-irradiation. The present results on adenine suggest, as reported previously for thymine, that a specific oxidative species is produced from water in beta-radiolysis but not in gamma-radiolysis.View full abstractDownload PDF (372K)
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