巻号一覧
17 巻, 10 号
選択された号の論文の9件中1～9を表示しています
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IV. 酸沈澱物質の一分画と菌体ならびに莢膜成分との抗原関係高木 静雄, 広直 武司1962 年 17 巻 10 号 p. 817-821
発行日: 1962/10/25
公開日: 2011/06/17
ジャーナル フリーA fraction (F-3) which precipitated in higher titer with anti-B. anthracis serum was separated from the acid-precipitating substance which had been produced in culture fluids of B. anthracis and some exper-iments were perfurmed on the fraction for the purpose of elucidating whether it was identical with the capsular polypeptide or one of the degradation products of the cellular substances. Fraction F-3 was a substance which differed from the capsular polypeptide not only in amino acid composition but also in serological activity. The paper chromatographical analysis showed the presence of six or seven amino acids and two unidentified ninhydrin-positive components in the hydrolyzate of fraction F-3 and the presence of glutamic acid and a trace of alanine in the hydrolyzate of the capsular polypeptide. Fraction F-3 precipitated in high titer when anti-B. anthracis serum was used, but the capsular polypeptide gave no precipitate with the same antiserum.
Fraction F-3 was serologically closely related to the acid-precipitating substance isolated from the crude extract of living cells of B. anthracis.抄録全体を表示PDF形式でダウンロード (1478K) -
(II) 各種動物大喰球の抗菌性について大野 治俊1962 年 17 巻 10 号 p. 822-828
発行日: 1962/10/25
公開日: 2011/06/17
ジャーナル フリーThe results of typical experiments in hostparasite relationships at cellular level, using staphylococci and Salmonella enteritidis to mononuclear phagocytes systems, have been presented.
The intracellular destruction of staphylococci in normal rabbit, guinea pig, rat, mouse derived from each peritoneal cavity was observed, and each pathogenicity was differentiated by each cellular levels.
The intracellular multiplication of Salmonella enteritidis in normal mice, guinea pig and rat mononuc-lear phagocytes was observed.
In Salmonella enteritidis to rabbit mononuclear systems the multiplication of the organisms was significantly restricted.抄録全体を表示PDF形式でダウンロード (3623K) -
抗血清被動免疫マウスにおける種々の経路からの攻撃実験 (死菌免疫効果の機序)秋山 武久, 前田 勝利, 牛場 大蔵1962 年 17 巻 10 号 p. 829-834
発行日: 1962/10/25
公開日: 2011/06/17
ジャーナル フリーWhen mice passively immunized with anti-O serum were challenged intravenously by virulent organisms of Salmonella enteritidis, a slight inhibition of bacterial multiplication could be observed only in the blood, and the bacteria in the liver and spleen multiplied as rapidly as in controls. The antilethal effect of the passive immunization was recognized only against an intravenous challenge with a small dose (5×10-7 mg) of bacteria.
When challenge infection was carried out through the subcutaneous (10-4 mg bacteria) or per oral (2-4 mg) routes, no inhibition of bacterial multiplication was noted at all either inregional lymph nodes or in liver and spleen of the immunized mice.
The hitherto obtained deta concerning the effect of killed vaccines in experimental typhoid of mice, which was shown to be antilethal only when immunized mice were challenged intraperitoneally, have been discussed with the above-mentioned results.
Since the marked clearance phenomenon by antibodies could be observed only in the peritoneal cavity, it seemed reasonable to assume that an antilethal effect was obtained when the clearance was so extensive that very few bacteria could escape from loco to organs. On the contrary, after challenge through various routes other than peritoneal cavity, bacteria, not receiving marked “clearance” effects at loco, easily multiplied in organs of the immunized mice.抄録全体を表示PDF形式でダウンロード (1284K) -
2. 感作動物の腹腔細胞によるPassive transfer浅見 望, 片岡 哲朗, 三浦 馨1962 年 17 巻 10 号 p. 835-838
発行日: 1962/10/25
公開日: 2011/06/17
ジャーナル フリーIn order to analyse the intradermal reaction between human leprosy bacilli and murine leprosy bacilli, human leproso bacilli and tubercle bacilli, murine leprosy bacilli and avian tubercle bacilli, experiment for passive transfer was performed, by using peritoneal cells with hypersensitivity to tuberculin type in guinea pigs.
Results were obtained as follows:
1. Peritoneal exudate cells from sensitized donor guinea pigs of human leprosy bacilli, murine leprosy bacilli, tubercle bacilli and avian tubercle bacilli were capable of transfer to normals recipients. The intradermal reaction of recipients were a similar specificity to that of donor.
2. The specificity of human leprosy bacilli was different from that of murine leprosy bacilli.
3. The human leprosy bacilli had a similar specificity to that of tubercle bacilli.
4. The specificity of murine leprosy bacilli was essentially the same as that of avian tubercle bacilli.抄録全体を表示PDF形式でダウンロード (758K) -
続報1. 抗酸菌フアージの菌体への吸着および増殖に及ぼす表面活性剤の影響川原 弘1962 年 17 巻 10 号 p. 839-843
発行日: 1962/10/25
公開日: 2011/06/17
ジャーナル フリーIn order to reveal the host-mycobacteriophage relationships, the effects of four kinds of surface active agents on adsorption and multiplication of mycobacteriophage were examined. All experiments have been made using Jucho-B1 system as host- phage system.
Non ionic surface active agent (Tween 80) inhibited adsorption of the phages to the bacilli and multi- plication of the phages. It is thought that inhibition of multiplication of this phage is caused by that of adsorption of the phage to the bacilli. Cationic surface active agent (Acetamine 24) inactivated free phage, but had little effect on adsorption and multiplication. Anionic surface active agent (Emahl O) inactivated free phage and inhibited adsorption, but did not multiplication. This is due to promotion of bacterial growth by Emahl O. Dual ionic surface active agent (Cellogen F) had no effect on free phage and adsorption of B1 phage, but exhibited a weak inhibition of multiplication.
Calcium ion promoted adsorption of micobacteriophage B1 to the bacilli.抄録全体を表示PDF形式でダウンロード (1021K) -
新井 武利1962 年 17 巻 10 号 p. 844-848
発行日: 1962/10/25
公開日: 2011/06/17
ジャーナル フリーIn the cultivation of El Tor vibrio Denken strain, in liquid media, the bacterial growth and hemolysin production are not parallal in various composition of culture media. In there sorts of synthetic media, Casamino acids medium, medium containing 18 amino acids, and medium containing 8 amino acids, 8 amino acids medium is best in the hemolysin production per unit amount of cells.
To investigate the mechanism of this phenomenon, the Fe content of these media was measured according to the O-phenanthroline method; but, the difference of Fe content in these three media was not so definite as to explain the reason of above mentioned phenomenon. Then, after removing the total Fe in the three sortes of media, FeSO4·7H2O was added in various amount to these media, and relation between the bacterial growth and hemolysin production was investigated, but the effect of Fe on the hemolysin production was not clear; the addition of FeSO4·7H2O in 50 γ/ml to 8 amino acids medium seemed somewhat helpful for the hemolysin production.抄録全体を表示PDF形式でダウンロード (834K) -
須子田 キヨ, 弥吉 真澄1962 年 17 巻 10 号 p. 849-856
発行日: 1962/10/25
公開日: 2011/06/17
ジャーナル フリーIn this study a comparison of agar-diffusion precipitin and γ-globulin in the serum of rabbits infected with mycobacterium and immunized with dead mycobacterium was carried out. The γ-globulin was tested by means of paper electrophoresis. The γ-globulin in the serum of immunized rabbits increased following higher ithmunization, and agar-diffusion precipitin gave positive results also, while globulin in the serum of rabbits inoculated with living mycobacterium increased during early infection, but agar-diffusion precipitin seemed to be produced later. The bands of precipitate made by the agar-diffusion precipitation test between the serum of rabbits infected with mycobacterium and the antigen derived from mammalian tubercle bacilli and atypical mycobacteriUm, indicated a confluency with the band which was produced by the reaction between the serum of rabbits immunized with dead mycobacterium and antigen mentioned above. Therefore, there is no doubt that the agar-diffusion precipitin in the rabbits sera infected with mycobacterium and sera immunized with dead mycobacterium is qualitatively equal, even though quantitatively somewhat different. Some rabbits injected intratesticularly with living human tubercle bacillus H37Rv showed a marked pathological change and an increase of γ-globulin and also a positive agar-diffusion reaction.抄録全体を表示PDF形式でダウンロード (6559K) -
浅見 望, 神薗 稔, 清水 裕子, 片岡 哲朗1962 年 17 巻 10 号 p. 857-861
発行日: 1962/10/25
公開日: 2011/06/17
ジャーナル フリーThe kinds and methods of preparation of adjuvants for sensitization to PPD were experimented in guinea pigs.
Results: As adjuvant emulsion of arlacel A and bayol F, arlacel A and drakeol and arlacel A and Krearol were capable of the similar sensitization. But emulsion of paraffin oil and anhydrous lanoline were less effective. The proportion of arlacel A and bayol F was best in 1: 9 (volume/volume). Inoculation with 2mg or 0.2mg of PPD had capacity of the similar sensitization. Emulsion of PPD with Tween 80 were lower sensitive than PPD without Tween 80.抄録全体を表示PDF形式でダウンロード (787K) -
第1 報ツベルクリン活性ペプチドの抗原性について染谷 四郎, 林 治1962 年 17 巻 10 号 p. 862-866
発行日: 1962/10/25
公開日: 2011/06/17
ジャーナル フリーThere have been many reports on the antigenicity to induce hypersensitivity of the tuberculoprotein in experimental animal. It is still a matter of controversy, however, whether the hypersensitivity induced by the administration of tuberculoprotein is of the delayed tuberculin type or of the anaphylactic type.
In the present studies, it was attempted principally to test the antigenicity of various kinds of tuberculoproteins and tuberculin active peptide in the induction of delayed type hypersensitivity in guinea pigs. The following results were obtained.
It was observed that the guinea pigs sensitized with purified tuberculin proteins (PPD-S and π) did not show any sign of hypersensitivity at 4 hours after skin testing but developed a marked positive response to 1.0 γ PPD-S at 24 and 48 hours. Therefore, all of these reactions are considered to be of the delayed type in their time of onset
Morever, relatively large amounts of extremely pure tuberculin active peptide in water-in-oil emulsion was not able to induce either immediate or delayed type hypersensitivity during the whole period of 10 weeks after the inoculation of tuberculin active peptide.
Accordingly, it is suggested that the ability of various kinds of purified tuberculoprotein derivatives to induce tuberculin type hypersensitivity reported by many researchers was either due to the contamination of lipopolysaccharide and other bacillary constituents or the antigenicity of protein itself, while tuberculin active peptide had no or very low, if any, antigenicity to induce delayed type hypersensitivity.抄録全体を表示PDF形式でダウンロード (662K)
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