All issues
Volume 17, Issue 12
Displaying 1-8 of 8 articles from this issue
- |<
- <
- 1
- >
- >|
-
Minoru SHIROTA, Kenji ASO, Akira IWABUCHI1962 Volume 17 Issue 12 Pages 917-927
Published: December 25, 1962
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSIt was revealed that GYB (glucose yeast broth) agar devoid of calcium carbonate was best fitted for counting the total number of viable bacteria in feces, regardless of their kinds. Widely used TGC (thioglycollate) broth was found far inferior to GYB-agar as regards fo: accuracy and sensitivity.
Quantitative estimation and isolation of lactobacilli from human feces were performed most excellently when LBS (Lactobacillus selective medium) 1 agar was used under anaerobic condition. IBS 1 agar is a modified medium of Rogosa's medium (1951), developed by the authors in order for better isolation of lactobacilli. It contains, besides LBS medium, liver extract, lactose and cysteine.
As for estimation of lactic acid bacteria (lactobacilli and streptococci), our tomato juice agar containing 0.1% furan-acrylic acid gave most excellent results.
Concomitant studies on various selective media currently used for other microbes confirmed that for enteric bacteria, MacConkey, Desoxycholate agar, SF (Streptococcus faecalis) medium as well as Staphylococcus medium No.110; for yaests and Candida, potato glucose agar or CGS (Sabouraud's medium containing guanoflacine) agar; were excellent selective media, respectively.View full abstractDownload PDF (1618K) -
V. The effects of BCG immunization and SM treatment on the guinea pigs infected with oil-coated tubercle bacilliSukeyoshi KUDOH1962 Volume 17 Issue 12 Pages 928-931
Published: December 25, 1962
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSIn the previous reports, the author presented repeatedly the marked phenomenon that the non-yathogenic acid-fast bacteria, such as M. phlei, produce severe lesions on various animals, when the bacteria are coated with fat or oil.
In this paper, some experiments are reported which were performed to explain the mechanism of the above phenomenon.
Both the BCG immunized and SM treated animals were infected by oil-coated and water-suspended tubercle bacilli. It was revealed that the oil-coated tubercle bacilli are protected from the effect of BCG immunization and SM treatment. It is considered, however, that the cause of this phenomenon is not only the protection by the oil-coating but the other factors.
Furthermore, from the view-point of this phenomenon, the author discusses about the so-called atypical mycobacteria isolated in Japan.View full abstractDownload PDF (679K) -
2. Mechanisms of the Intracerebral Infection with B. pertussis in miceShiro SOMEYA, Yoshinori KANEKO, Naomichi KASAI1962 Volume 17 Issue 12 Pages 932-937
Published: December 25, 1962
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSMany workers reported on the experimental studies on pertussis by the intraperitoneal, intranasal or intracerebral routes of infection. Although the mice could be killed when they were injected intraperitoneally and intranasally with large amounts of B. pertussis, the death would be principally caused by the toxicity of the inocula itself and not infection. Therefore, the intracerebral infection in mice has been generally used in experimental studies of pertussis immunity and the protection test of pertussis vaccine.
In the present studies it was intended to examine the multiplication of B. pertussis (phase I: 18-323, variant:#33 and phase III: Sugata) inoculated intracerebrally and its relationship to the death of infected mice, and also the death of mice inoculated intracerebrally with various doses of killed organisms of five strains of B. pertussis /phase I: 18-323, Tohama; variant:# 33, # 29; phase III: Sugata. And the results obtained are as follows;
Mice inoculated intracerebrally with lethal doses of 18-323 and # 33 strains of B. pertussis showed a marked rise in the number of viable organisms in the brain at 5 to 7 days after infection. All mice die within 4 to 7 days after infection showing the microbial populations of 108 to 1010 viable units in the brain. The number of viable organisms in the brain of each mouse, which did not show any symptoms of meningitis or encephalytis, was under 10 viable units at 5 to 7 days after infection and thereafter a striking reduction of microbial populations was observed. On the other hand, mice inoculated with B. pertussis of phase III showed a remarkable decrease of viable organisms in the brain during the entire observation period and all mice survived.
The ability of killed B. pertussis of various phases was compared at doses of 107, 108 and 109 organisms. Death rate of each group of mice inoculated with 109 organisms of phase I and variant strains of B. pertussis was 80 to 100 per cent respectively, and 107 and 108 organisms of these strains killed no or only a few mice. Moreover, mice inoculated with 107, 108 and 109 organisms of phase III B. pertussis did not die within 72 hours after inoculation.
From the above mentioned findings, it will be concluded that the death of mice infected intracerebrally with lethal dose of virulent B. pertussis is due to the toxin produced in the brain as the result of the growth of organisms to the level of 108-109 viable units.View full abstractDownload PDF (1142K) -
Report 3. A study on the inhibitory action of Radix liquiritiae fractions on bacterial growth and the increased potential of resistance in bacteriaDaiji MANO1962 Volume 17 Issue 12 Pages 938-941
Published: December 25, 1962
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSAfter extracting Radix liquiritiae from Ether, Acetone by ethanol, it was further fractionated into. three R. liquiritiae fractions, (A), (B), and (C). With these fractions a series of experiments was conducted to find out the inhibitory action of these fractions on the bacterial growth as well as the increased potential in the bacterial resistance against these fractions and compared the same of Allium sativum fraction (A). Original Staph. aureus 209 P, PC, SM-resistant strains and PC-SM double resistant strain served as the bacterial materials. The results of the study are summarized in the following.
I. R. liquiritiae fraction (A) has been found to show the inhibitory action on the original strain of Staph. aureus 209 P and its drug resistant strains only, but (B) and (C) fractions show no effect on the bacteria. To other strains of bacteria the fractions (A), (B), and (C) do not show any inhibitory effect. However, R. liquiritiae fraction (A) shows its inhibitory action on the growth of Staph. aureus 209 P even at the concentration of 1/5 that of A. sativum fraction.
2. In the successive tissue culture of four strains, the original strain, PC, SM- drug resistant strains of Staph. aureus 209 p in the media containing R. liquiritiae fraction (A) and A. sativum fraction (A), it has been found that the increase in the resistance potential against A. sativum fraction (A) is considerably different between the original strain and the drug resistant strains of Staph. aureus 209 P, but that against R. liquiritiae fraction (A) is considerably potentiated in both the original and the drug resistant strains. In other words, at the tenth generation, the original strain shows the 6-fold resistance, while PC, SM-resistant strains 8-fold resistance and PC-SM double resistant strain 10-fold against A. sativum fraction (A). On the other hand, R. liquiritiae fraction (A) imparts the resistance to both the original strain and the drug resistant strains in the same degree, namely, only 3-fold resistance at the tenth generation of the successive tissue culture.View full abstractDownload PDF (668K) -
Superinfection of a host bacterium with two R factorHajime HASHIMOTO1962 Volume 17 Issue 12 Pages 942-952
Published: December 25, 1962
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSE. coli 0-26 and E. coli K-12 were superinfected with three types of R factor: R (CM), R (TC) and R (SM). When R (CM) or R (TC) factor was superinfected to R+ (TC) or R+ (CM) cell, interferrence between the two factors was always found, i. e. 1) decrease of transmission frequency of R factor, comparing with the case that R-cell was used as a recipient, 2) substitution of the two R factors, 3) unstable existence of doubly infected R factors.
Between R (SM) and R (TC) or R (SM) and R (CM) factor, interference between the two R factors was not found. Doubly resistant cells were easily obtained, indicating that the double resistance (SM. TC or SM. CM) was stable after successive subculturieg and the cells carried two types of R factor (R (SM) and R (TC), or R (SM) and R (CM)).
But one of the two R factors was transferred segregately to recipient by conjugation, suggesting that the two R factors existed separately in a host bacterium. When R (TC) and R (CM) factors were doubly infected to a host bacterium, (CM. TC) resistant cells were obtained. As the result of interaction between the two types of R factor present in a host bacterium, recombinant factor was obtained R31 (CM. TC). The recomb inant factor was able to transfer its resistance by conjugation and was also transduced as one unit into E. coli K-12 by PIkc phage.View full abstractDownload PDF (2130K) -
1962 Volume 17 Issue 12 Pages 953-964
Published: December 25, 1962
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSDownload PDF (2493K) -
1962 Volume 17 Issue 12 Pages 965-989
Published: December 25, 1962
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSDownload PDF (5570K) -
1962 Volume 17 Issue 12 Pages 990-994
Published: December 25, 1962
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSDownload PDF (940K)
- |<
- <
- 1
- >
- >|