巻号一覧
17 巻, 6 号
選択された号の論文の14件中1～14を表示しています
- |<
- <
- 1
- >
- >|
-
第4報数種酵素処置の鼡癩菌感染力に及ぼす影響中村 昌弘1962 年 17 巻 6 号 p. 387-390
発行日: 1962/06/25
公開日: 2011/06/17
ジャーナル フリーThe effects of various enzymatic digestions on the infective activity of Myc. lepraemurium were investigated. Each enzyme powder was resolved to give a concentration of 0.2% in a 10% bacterial suspension, and was incubated for several hours with occasional agitation at 37°C in the bath.
The Kumamoto strain of Myc. lepraemurium was used for these experiments and the enzymes employed were as follows: trypsin, papain, ficin, diastase, lipase, protamylase, ribonuclease, pronase and lysozyme. One tenth ml of each bacterial suspension containing enzyme was subcutaneously inoculated in mice at 1, 3, and 5th hour after incubation, respectively. Mice tested were killed after 2 or 5 months of inoculation, and the infectious activities of the bacilli treated with enzyme were determined by microscopical examinations. The results.obtained demonstrated that Myc. lepraemurium was not inactivated by the treatment with trypsin, papain, ficin and diastase for five hours at 37°C, however, it was inactivated by other enzymes tested within five hours, especially lipase inactivated clearly the infectious activity of Myc.lepraemurium within three hours at 37°C. These results would contribute to the studies regarding to bacterial compositions and isolation procedureof this bacillus from the infected tissues.抄録全体を表示PDF形式でダウンロード (600K) -
II. 成立条件の追究佐藤 久蔵, 岡田 和夫, 菊田 和子, 竹島 竹, 天田 賢1962 年 17 巻 6 号 p. 391-397
発行日: 1962/06/25
公開日: 2011/06/17
ジャーナル フリーThe results obtained from the interfernce tests in mice, guinea-pigs and rabbits were similar to each other, with the evidence that each of the animals was protected against the challenge with more than lethal doses of Bacterium tularense, when they were injected with avirulent or inactivated strains of the organisms one or three days before the challenge. Such early protection occurred prior to the production in animal body of agglutininis and protective antibodies and according to Kitamura et al., the histological pictures were principally the same with those observed in the case of interference phenomenon with ectromelia virus. Furtheremore, Kitamura et al., on the bases of their histopathological findings, proposed that the interference phenomenon was different from the immune phenomenon and it manifested itself as the form of weakened infection. This findings seemed to be harmonious with our findings obtained by observing the change in number of the challenge organisms in the bodies of immunized, interfered and control animals. In immunized animals the challenge organisms disappeared with the lapse of time after challenge and all the animals could survive the challenge. The interfered animals held the challenge organisms for a considerable longer time as compared with immunized animals and were alive for a longer time than the control some of them surviving the challenge. In control animals, the challenge organisms multiplied in their body representing a linear growth curve and killed all the animals by the same dose as was used in the case of immunized and interfered animals.
In the successful demonstration of the interference phenomenon, there was a certain quantitative relationship between interfering agent and challenge agent and that the subcutaneous injection of both the agents was found to be best suited for this purpose. This interference phenomenon is non-specific phenomenon and interfering organisms were not influenced by physical and chemical treatments.抄録全体を表示PDF形式でダウンロード (978K) -
第9報C3H系とddY系間の初代雑種マウスにおける鼡癩重感染実験川口 陽一郎1962 年 17 巻 6 号 p. 398-401
発行日: 1962/06/25
公開日: 2011/06/17
ジャーナル フリーAs stated in the preceding report, agouti mice of (C3H×ddY) Fl were characteristic in their response to subcutaneous inoculation with murine leprosy bacilli, roughly half of them being of malignant type and the rest being of benign type of mouse leprosy. However, when these Fl hybrids had been vaccinated with BCG, challenge lesions took the feature of benign type in all cases. This observation led us further to the study on the influence of primary infection with murine leprosy bacilli, instead of BCG, upon the leproma development of superinfection in these F1 hybrids.
Mice of (C3H×ddY) F1 were infected subcutaneously with murine leprosy bacilli. Superinfection was made by subcutaneous inoculation 16 weeks later only in the mice of these F1 hybrids, showing malignant leproma at the site of primary infection.
About half of superinfected mice showed intermediate but rather benign leproma at the challenged site. In the remaining mice, however, the lesions of superinfection were malignant or intermediate but nearer to malignant type. Thereupon, the typical benign leproma could not be observed in all experimental animals.
It may be stated as a conclusion from the result of this experiment that murine leprosy primary infection can not convert the superinfection from the malignant type to the benign type unlike BCG vaccination.抄録全体を表示PDF形式でダウンロード (620K) -
II. 内毒素の感染増強作用について中野 昌康, 斎藤 和久, 沖津 冨士夫, 牛場 大蔵1962 年 17 巻 6 号 p. 402-407
発行日: 1962/06/25
公開日: 2011/06/17
ジャーナル フリーInfection-enhancing effect of purified lipopolysaccharide (endotoxin) from S-type Salmonella enteritidis was clearly observed even when its very small doses were given to mice intraperitoneally together with low-virulent R-type S. enteritidis. This effect of endotoxin was detected to a comparable extent also after several days following infection, but thereafter reduced gradually.
Lipopolysaccharide fractions extracted from various mutants of S. enteritidis and Proteus OXK also showed the property of enhancing the infection with low-virulent S. enteritidis. This infection-enhancing effect of endotoxin was not reduced at all by the previous administration of antiserum to mice. The acidhydrolysate of endotoxin also showed infection-enhancing effect.
Mechanisms of this effect of endotoxin were studied by following the distribution of infecting32Plabeled bacteria and the changes of cells counts in peritoneal fluid. Although the phagocytic activity of peritoneal exudate cells was not affected, if any, by administration of endotoxin, the bactericidal activity of them was markedly reduced. A majority of the bacteria were not killed in the peritoneal cells of mice which received endotoxin, tnd gradually multiplied in them. Total counts of peritoneal exudate cells markedly decreased after administration of endotoxin, while no remarkable chages of their differential counts occured. It was concluded that endotoxin enhanced bacterial infection by reducing the mobilization of peritoneal exudate cells as well as damaging their bactericidal properties rather than by affecting their phagocytosis.抄録全体を表示PDF形式でダウンロード (850K) -
林 長男, 岩原 繁雄1962 年 17 巻 6 号 p. 408-412
発行日: 1962/06/25
公開日: 2011/06/17
ジャーナル フリーThe methods of administration in this experiment conformed to R. Freter's Shigella flexneri type 3a of logarithmic stage were administered directly into the stomach of guinea pigs. In the first experiment, animals were sacrificed at 1, 2, 3, 4, 6, 8, 24, 48 and 72 hours. Then, these animals were dissected and the number of dysentery bacilli in the gastrointestinal tract were examined by cultivation.
1) 113×108 live organisms were administered to each of 9 animals. These organisms passed through stomach and small intestine very quickly, and reached to the large intestine in one hour. After 72 hours, numerous dysentery bacilli were detected from the distal ileum to the distal rectum.
2) 18×106 live organisms were administered to each of 7 animals. These organisms reached to the proximal part of the large intestine in one hour. After 4 hours, dysentery bacilli were detected only in the large intestine of these animals. After 24 hours, these organisms disappeared from the gastrointestinal tract.
In the second experiment, two groups of animals were used. One group was nourished witth “Sunmeal” and the other with “Oriental MF”.Dysentery bacilli were administered in the animals with the techniques described above. Animals were sacrified day about. These animals were dissected and the total live dysentery bacilli in the gastrointestinal tract were counted by cultivation.
1) 5 animals on “Oriental MF” were administered 50×108 live organisms. Even after 7 days, these animals had 104-105 of total live organisms in the large intestine.
2) 4 animals on “Sun-meal” were administered 25×106 live organisms. Dysentery bacilli were detected in the large intestine up to 3 days.
3) The elevation of serum-antibody titre was observed (max.×32). But copro-antibody tests were negative.抄録全体を表示PDF形式でダウンロード (816K) -
第2報酵母エキス中に不足しやすい成分入江 良三郎, 矢野 信礼, 森地 敏樹, 見坊 寛1962 年 17 巻 6 号 p. 413-416
発行日: 1962/06/25
公開日: 2011/06/17
ジャーナル フリーGrowth supporting abilities of yeast extract-glucose broth prepared from various commercial yeast extract samples for L. blugaricus were compared.
There were considerable differences in the growth supporting ability among the lots of yeast extracts and in some cases, only very poor growth was obtained. It was considered that in these media, some of the growth factors reported in the preceding paper were insufficient to obtain normal growth.
Additions of “tween 80” and panthetine markedly improved the growth and heavy growth was obtained irrespective of the quality of yeast extract used.
Pantethine could be replaced with 0.01 per cent of cysteine. An explanation for this result is, as discussed in the preceding paper, that pantothenic acid which is present in yeast extract medium shows some pantethine activity only in the presence of relatively large amount of cysteine.
At first, the nature of “tween 80” active substance (s) in yeast extract was considered to be phospholipid (s) such as lecithin, since egg lecithin supported growth when added in place of “tween 80”. However, this substance (s) could not be extracted with 95 per cent ethanol and ethanol-ether mixture.抄録全体を表示PDF形式でダウンロード (537K) -
第1報黄色葡萄球菌および腸内細菌の発育阻止効果, Penidllin, Streptomycinの人工耐性菌について真野 大二1962 年 17 巻 6 号 p. 417-421
発行日: 1962/06/25
公開日: 2011/06/17
ジャーナル フリーWith the use of extracts of three kinds of plants (Lycoris radiata, Narcissus tazetta, and Allium sativum) diluted with neutral bouillon the author studied antagonistic action of various strains of bacteria to the dose of 0.1 mg of these extracts and obtained the following results: Namely, the extracts of Lycoris radiata and Narcissus tazetta did not show any inhibitory action on the growth of bacteria while the extract of Allium sativum demonstrated very strong inhibitory effect.
1. The extract of Allium sativum at the concentration of 1-5 mg/ml showed an inhibitory action on the growth of susceptible bacteria; 6 strains of Staph. aureus, 3 strains of Candida albicans, 5 strains of E. coli, 3 of Proteus and one of Sh. flexneri, to the total of 18 strains, and the extract at the concentration of 10-50 mg/ml showed a complete inhibitory effect on the growth of 6 strains of Ps. aeruginosa.
2. It was also recognized that 16 strains of Staph. aureus exposed to 1, 000 μ/ml of PC and 10, 000 γ/ml of SM and 3 strains of E. coli exposed to 1, 000γ/ml of SM, the total of 19 strains of resistant bacteria, revealed inhibitory effect on the growth the same as in the case of susceptible strains.
3. In the experiments conducted to see whether or not a reinforced inhibitory effect could be attained with the mixture of the extract of Allium sativum combined with phenol, there could be observed no such reinforced effect on the growth.
As can be seen from these findings, some plant extracts do possess inhibitory action on the growth of certain strains of bacteria and it is an interesting problem which requires further studies.抄録全体を表示PDF形式でダウンロード (755K) -
各種合成培地の増殖力の比較および安士培地の改良西村 七郎1962 年 17 巻 6 号 p. 422-427
発行日: 1962/06/25
公開日: 2011/06/17
ジャーナル フリーI have investigated the growth supporting abilities of three synthetic media for the growth of S. pullorum and S. gallinarum Used strains were 35 strains of S. pullorum and 11 of S. gallinarum Tested synthetic media were those reported by Yasushi (1959), Schoenhard and Stafseth (1953) and it's modification by Gilfillan et al.(1955).
Many of the used strains of S. pullorum could grow moderately in the composition of Yasushi, but in the other two media only six strains showed good growth, the growth of the others were scanty or none.
The reason of this difference of growth response was explained by the fact' that many strains of S. pullorum required proline and glutamic acid for their growth, and the two mediaother than Yasushi's lack these amino acids.
All tested strains of S. gallinarum except one showed good growth in three media. Therefore, the growth requirement of S. gallinarum seems more non-exacting than S. pullorum.
5 strains of S. pullorum could not grow fairly in the Yasushi's composition, and a modification of Yasushi's medium was attempted. As the results, it was confirmed that the addition of histidine and methionine, increase of the added amount of glucose were effective for the increase of growth.
One strain of S. pullorum, strain No.21, could not grow even in the modified composition of Yasushi's medium, the growth requirement of which must be investigated in future.抄録全体を表示PDF形式でダウンロード (832K) -
有効物質の同定 (1)榊原 栄一, 萩原 一成, 阪本 正子, 上延 冨久治, 広尾 哲三1962 年 17 巻 6 号 p. 428-433
発行日: 1962/06/25
公開日: 2011/06/17
ジャーナル フリーThis experiment was tried to extract and purify the substance which accerelatesboth the promotion and production of antibody and to determine its molecular structure
At first, according to the method reported in the previous report, a crude effective substance was extracted by the treatments with Amberlite IR 120 H+ resin and hydrochloric acid in serum which was separated from the rabbit's blood in the early stage after the second vaccination of typoid vaccine containing O antigen.
Secondly, the crude substance was repeatedly treated, taking the following procedures: i. e. it was dissolved in water, absorbed with an active animal carbon-powder and filtrated, then condensed and finally was purified into a needle crystal.
Thirdly, the physical and chemical properties of the substance thus purified coincided completely with the characters of the substance which had been reported several times before. Nevertheless it did not exhibit the promoting and producing actions of antibody. From this fact, the purified substance could not be determined to be the very object. But it was determined to be “hypoxanthine” as the results of colorimetriclphenomena, protein-reactions, elementary analyses, paperchromatograms and spectro-photometric measurements by ultraviolet- and infrared-ray, etc.抄録全体を表示PDF形式でダウンロード (811K) -
第1報諸種の処置による赤痢菌のマウス感染成立の試み岩田 和夫, 本江 博子, 福永 昇1962 年 17 巻 6 号 p. 434-442
発行日: 1962/06/25
公開日: 2011/06/17
ジャーナル フリーA continuous oral administration of antibiotics, mostly chloramphenicol, in combination with resistancelowering agents, such as carbon tetrachloride, cortisone acetate and croton oil, was very effective in establishing infections in mice when they were challenged with resistant strains of Shigella 2b and 3a. The inoculated organisms remained in the intestinal tract for about a week in most of the animals and for as long as 4 or 5 weeks in a few. Pathologic examination revealed that lesions developed in various organs, especially the intestinal tract, showing acute or chronic colitis.
Infection was not induced in animals which were administered sensitive strains; in most of the mice injected with the sensitive strain, no organisms could be found in their feces after 1 or 2 days. The absence of organisms in the feces may be attributed to the difference in treatment of mice with antibiotics; the antibiotic was administered for 2 or 3 days and stopped one day prior to the inoculation of organisms.
Thus, the elimination of intestinal microbial flora with the aid of antibiotics is primarily significant in establishing Shigella infection in mice and that the resistance-lowering agents seem to play a secondary role, in accelerating the infection.
Differences in the serotype of Shigella (2b and 3a) and in the sex (male and female) and strains (dd and CFW) of mice did not seem to influence the grade or course of infections as far as the results obtained were concerned.抄録全体を表示PDF形式でダウンロード (3396K) -
第1報L細胞の抗酸菌食菌に影響を与える血清の種類について中村 昌弘, 志方 弘之1962 年 17 巻 6 号 p. 443-447
発行日: 1962/06/25
公開日: 2011/06/17
ジャーナル フリーThe effects of sera on the infection of Mycobacteria to the established cell derived from mouse were investigated in vitro. The cells used were L cell which was derived from mouse fibroblast and HeLa cell, and the strains of Mycobacteria employed were Myc. tuberculosis hominis H37Rv, H37Ra, H37Rv-INHresistant, STM-resistant, Myc. tuberculosis bovis BCG and saprophytic mycobacteria smegmatis, respectively. The following results were obtained.
1. There were some sera having different effects in the course of phagocytosis of Mycobacteria by the L cell and HeLa cell.
2. In general, it was demonstrated that the guinea pig serum promoted phagocytosis of Mycobacteria by the L cell. This promoting effect of the serum might be due to the complement-like substance because the effect was inactivated by heating at 60°C for 30 min.抄録全体を表示PDF形式でダウンロード (3735K) -
川原 弘1962 年 17 巻 6 号 p. 448-452
発行日: 1962/06/25
公開日: 2011/06/17
ジャーナル フリーIn order to find any distinctive marker among three mycobacteriophages, A6, B1 and C3, which have different host ranges, respectively, the properties of thermostabilities, ultraviolet- and pH stabilities of these phages were examined and the results obtained were summarized as follows:
1. A6 phage was the most thermolabile of three phages studied. B1 and C3 phages were thermostable as compared with A6 phage. At high temperature, B1 phage was more stable than others.
2. B1 phage was easily and promptly inactivated and A6 phase was gently and gradually inactivated by ultraviolet irradiation.
3. In general, three phages were stable in alkali, and labile in acid, but in alkali solution, C3 was relatively stable, and in acid solution, A6 was stable, respectively, among the phages.抄録全体を表示PDF形式でダウンロード (700K) -
1962 年 17 巻 6 号 p. 453-468
発行日: 1962/06/25
公開日: 2011/06/17
ジャーナル フリーPDF形式でダウンロード (3094K) -
1962 年 17 巻 6 号 p. 469-476
発行日: 1962/06/25
公開日: 2011/06/17
ジャーナル フリーPDF形式でダウンロード (1514K)
- |<
- <
- 1
- >
- >|