巻号一覧
18 巻, 10-11 号
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変法中西の培地 (TCBSカンテン培地) について小林 俊一, 榎本 省二, 坂崎 利一, 桑原 章吾1963 年 18 巻 10-11 号 p. 387-392
発行日: 1963/11/25
公開日: 2011/06/17
ジャーナル フリーIt was reported by Nakanishi (1963) that a new selective plating medium consisting of ox bile, -thiosulphate, citrate, lactose, sucrose and skim milk is suitable for the selective isolation of both cholera vibrio and pathogenic halophilic bacteria (Vibrio parahaemolyticus).
This composition was modified by the authors as follows and named TCBS agar medium peptone 10 g/l, yeast extract 5 g/l, Na. citrate 10 g/l, Na. thiosulphate 10 g/l, NaCl 10 g/l, Fe. citrate 1 g/l, ox bile (powder) 5 g/l, Na. cholate 3 g/l, sucrose 10 g/l, agar 15 g/l, B. T. B (0.2 % aqueous sol.) 20 ml/l, thymol blue (0.2 % aqueous sol.) 20 ml/l ; pH 8.6.
After 24 hours incubation on this medium, freshly isolated strains of cholera vibrio and sucrose fermenting strains ofVibrio parahaemolyticusproduce large, smooth and yellow-coloured colonies, and sucrose-non-fermenting strains blue colonies.
Gram positive bacteria, proteus and coli-group are strongly inhibited. Some strains of proteus can grow on this medium, but the colonies are so small and colorless that they are easily distinguishable.抄録全体を表示PDF形式でダウンロード (849K) -
第1報超薄切片によるCandida albicansの内部構造について岩田 和夫, 平田 恒彦1963 年 18 巻 10-11 号 p. 393-404
発行日: 1963/11/25
公開日: 2011/06/17
ジャーナル フリーBy means of ultrathin section and electron microscopic technic the fine structures ofCandida albicansin yeast phase grown on Sabouraud's dextrose agar were revealed. Fixation of specimens proved to be superior in revealing the fine structures using a 2 % potassium permanganate aqueous solution (at room temperature for 48 hours) than with an osmic acid solution. Embedding was done in a routine method. The results obtained were as follows:
The cell wall, relatively thick, consists of the three layers, two electron less dense ones divided by a dense interspace. The cytoplasmic membrane, very thin, is recognized.
The nuclear apparatus is surrounded by a continous double-membraneous layer with pores; The nucleolus-like structures are observed as an electron dense region.
The mitochondria with cristae mitochondriales are also surrounded by a continous double-membraneous layer as in the nucleus.
The endoplasmic reticulum seems to be connected with the outer layers of both nucleus and mitochondria. The structures resembling the lamella, potassium permanganophilic uniform granules and vacuoles are also observed inside the cytoplasm.抄録全体を表示PDF形式でダウンロード (19976K) -
室橋 豊穂, 吉田 幸之助1963 年 18 巻 10-11 号 p. 405-409
発行日: 1963/11/25
公開日: 2011/06/17
ジャーナル フリーVarious types of Mycobacteria were tested on their acid-fastness employing victoriablue simultaneous staining (a modification of Hallberg's procedure) and nitric acid alcohol of various compositions as the decolorizing agent. The results revealed that the nitric acid alcohol composed of a rather low concentration of nitric acid (0.1-0.5%) in 70% ethylalcohol was obviously stronger than 3% hydrochloric acid alcohol or 4-8% nitric acid alcohol (100%) in the decolorizing effect and resulted in the complete decolorization of the acid-fast stain of Saprophytic Mycobacteria and M. fortuitum within 20 minutes of rinsing. Noteworthy is that the decolorized cells by the acid-alcohol of the abovementioned composition are completely restainable by the ordinary acid-fast staining procedures employing 3% hydrochlonic acid alcohol or 4 % nitric acid alcohol (100%) as the decolorizer.
Seventy six strains of Saprophytic Mycobacteria were tested employing abovementiond procedure and 74 strains among them were completely decolorized. Remaining 2 strains which resisted to this procedure were confirmed as the non-photochromogens (Unclassified M.) by other biological properties.
This method seems to be practically available as a very simple test method in distinguishing saprophytic strains from other types of Mycobacteria.抄録全体を表示PDF形式でダウンロード (893K) -
1報非定型抗酸菌による免疫実験乾 晁1963 年 18 巻 10-11 号 p. 410-417
発行日: 1963/11/25
公開日: 2011/06/17
ジャーナル フリーThe present paper describes the study on the virulence of various strains of unclassified mycobacteria and the comparison of protective effects for infection with human Mycobacterium tuberculosis between those strains and BCG.
1. Unclassified strains employed here were virulent for mice in the order of photochromogen P-16, non-photochromogen Ueda, scotochromogen Watanabe and non-photochromogen 121326.
2. When mice immunized with the above strains and BCG were challenged with M tuberculosis strain Kurono, the multiplication in the lung was apparently inhibited only in the mice immunized with BCG. However, the multiplication in the spleen was inhibited also in the groups of mice immunized with unclassified mycobacteria to the same extent as in the BCG-immunized ice. No difference in the degree of inhibition was noted between the mice immunized with strain Ueda and those immunized with strain Watanabe.
3. When the survival time of the challenged mice was compared, the mice immunized with strain Ueda showed a protective effect comparable to that by BCG immunization. The immunization with strain P-16 also conferred a protection, though weaker than that by BCG, but almost no effects were obtained by the immunization with strains Watanabe and 121326.
4. There was a clear parallelism between the in vivo multiplication of strains of unclassified mycobacteria and pathological findings in organs caused by them.
5. The mechanism of immunity conferred by unclassified mycobacteria was discussed and a possibility of its difference from the immunity by BCG has suggested.抄録全体を表示PDF形式でダウンロード (1627K) -
I. 感染初期における網内系の役割橋本 信也1963 年 18 巻 10-11 号 p. 418-427
発行日: 1963/11/25
公開日: 2011/06/17
ジャーナル フリーStudies on the role of the reticuloendotherial system (abbr. RES) in the early phase in mice infected with Salmonella were carried out.
Salmonella typhimurium as a virulent strain, Salmonella typhi as an avirulent strain and hybrid No.19 (a recombinant between Salmonella typhimurium and Escherichia coli), which is avirulent in mice but is antigenically identical with Salmonella typhimurium, were used. Intravenous injections of these trains did not show a significant difference in the uptake by the RES in the early phase of infection.
After previous treatment of the RES with heat-killed Salmonella typhimurium, living hybrid No.19 and thorotrast as a blocking colloidal material, the challenge of living Salmonella typhimurium into mice was employed. In the pretreatment with thorotrast it was clearly observed that the clearance of bacteria from the blood stream was depressed, the interval to the death by infection was shortened and a considerable amount of bacteria multiplied in the liver, the spleen, and the mesenteric lymphnode.
Using the carbon method as a RES function test, “blockade” was intensively observed in thorotrattreated mice.
The significance of the role that the RES plays in the early phase of infection, was discussed.抄録全体を表示PDF形式でダウンロード (1512K) -
1963 年 18 巻 10-11 号 p. 428-448
発行日: 1963/11/25
公開日: 2011/06/17
ジャーナル フリーPDF形式でダウンロード (4712K)
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