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Volume 18, Issue 8
Displaying 1-6 of 6 articles from this issue
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I. On Fluorescent Treponemal Antibody Test using the Reiter Treponeme (RFTA Test)Tatsuo SASAHIRA1963 Volume 18 Issue 8 Pages 335-343
Published: August 25, 1963
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSIn 228 problem sera, the results obtained by fluorescent treponemal antibody test using Reiter and Nichols strain of Treponema pallidum were compal ed and a high degree of correlation existed between the results obtained by both methods RFTA test was as quite specific for syphilis as FTA test.
It is evident that the complement method of fluorescent treponemal antibody test for syphilitic sera with anticomplementary action gave negative results and thus this method should not be used as a routine technique of FTA or RFTA.
The author devised a quantitative technique of RFTA test by using the 2-fold dilutions of syphilitic sera. It was shown that the RFTA titers of the same spcimens were always higher than the RPCF or reagin titers and furthermor the RFTA titers did not correspond to the FTA titers. Possible explanations for this result are discussed.View full abstractDownload PDF (1647K) -
Akio YAMAMOTO, Masako TSUKAMOTO, Ryosuke MURATA1963 Volume 18 Issue 8 Pages 344-347
Published: August 25, 1963
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSFactors influencing synthesis of alpha toxin by Cl. perfringens PB6K, were investigated by using a synthetic medium supplemented with a peptide (glycyl-L-asparagine) or a fraction derived from a protein. digest. Among various factors studied, amounts and kinds of phosphate, reducing agent and sugar seemed to affect greatly the yield of toxin. As a reducing agent, thioglycolic acid was by far superior to ascorbic acid for toxin production, while the latter was more suitable for growth. Regarding phosphate, a mixture. of Na2HPO4 and KH2PO4 was more favourable for toxin production than that of K2HPO4 and KH2PO4, while the latter supported a better growth. Fructose was preferable to sucrose or glucose so far as the yield of toxin was concerned.View full abstractDownload PDF (602K) -
Tohru TOKUNAGA, Yasuo MIZUGUCHI, Toyoho MUROHASHI1963 Volume 18 Issue 8 Pages 348-353
Published: August 25, 1963
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSPresented in this paper were the successful induction experiments of lysogenic mycobacteria, Myc. sp. Li (L1), by the use of ultraviolet light or hydrogen peroxide. Special sensitivity of this temperate phage to the different kinds of media was effectively utilized in the present experiments.
1. Phage Li released from Myc. sp. Li (L1) was rapidly inactivated in a M/15 phosphate buffer or physiological saline, but not in broth, distilled water and Tris buffer. Addition of more than 0.002 M CaCl2 prevented the inactivation. Inactivated phage Li was, however, not reactivated by the addition of Ca.
2. Myc. sp. L1 (L1) was successfully induced by the ultraviolet irradiation. Latent period after the induction was about 120-150 minutes, rise period about 150 minutes and burst size was calculated as about 5.
3. Myc. sp. L1 (L1) was successfully induced by hydrogen peroxide, too. Maximum induction rate was observed 8 minutes after the addition of hydrogen peroxide.View full abstractDownload PDF (1049K) -
Tomio KAWATA, Terutaka INOUE1963 Volume 18 Issue 8 Pages 354-361
Published: August 25, 1963
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSCells of clostrida, such as Clostridium tetani, Clostridium septicum and Clostridium butyricum, growing exponentially in a hypertonic sucrose-thioglycolate medium were quantitatively converted into osmotically fragile spheroplasts after addition of penicillin (1-1, 000 μ/ml) or glycine (1-3 %). The induction of spheroplasts occurred more rapidly at higher concentrations of the spheroplasting agents. In C. butyricum the rod cells transformed to irregular polyhedral forms as well as spheres.
The process of the spheroplast formation was followed by the electron microscope. Shortly after penicillin or glycine was added, the rod cells developed globular extrusions either centrally, subterminally or terminally. In C. butyricum the angular as well as globular extrusions were developed. The extrusions progressively enlarged to form spheres or irregular polyhedral forms (C. butyricum) and the retained portions of the rod attached to them withered away. The resulting spherical and polyhdral bodies, which appeared to have lost most parst of the cell wall, retained flagella but were non-motile.View full abstractDownload PDF (8219K) -
Yoshinori KANEKO, Fumiko MORIWAKI, Riichi SAKAZAKI, Shoji ENOMOTO1963 Volume 18 Issue 8 Pages 362-367
Published: August 25, 1963
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSIn the first part of this report the significance of blood constituents in tellurite medium was studied with the results that the blood corpuscles haemolysed by adding equal volume of sterile destilled water was sufficient for the growth of C. diphtheriae and the haemolysed corpuscles (HB) was effective to inhibit the growth of staphylococcus to the extent of 103.
Using HB as the supplement, heart infusion agar medium added with yeast extract to 0.5% was the most satisfactory base medium. In this case potassium tellurite can be increased to 0.05%.
The results of the clinical uses of this HB Medium are presented.View full abstractDownload PDF (909K) -
3. Booster Effect of the Challenge Dose in the Intracerebral Infection of MiceYoshinori KANEKO, Fumiko MORIWAKI, Shiro SOMEYA1963 Volume 18 Issue 8 Pages 368-373
Published: August 25, 1963
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSThe mouse protection test of pertussis antigen by the intracerebral method has been generally accepted as the most satisfactory method of assaying the potency on human bodies. However, the difficulty of this method is the reproducibility, mainly due to the variety of the virulence of the challenge dose.
In the minimum requirement of pertussis vaccine it is stated that the challenge culture should be of such virulence that the LD50 for the virulence control mice contains not more than 500 bacteria according to the plate colony count. From the viewpoint of reproducibility it is considered that this description of the challenge culture might not be enough from the following results.
In this report the results of the protection test by the original 18-323 strain and its substrain are reported. The original strain has been used in Japan without any selection of the colony on the plate for more than 10 years since the introduction from The United States. The LD50 of this strain in this report was 70 organisms by the plate count. The substrain was the strain, which was selected from the original strain on the plate and the LD50 was 10 organisms by the plate count. All of the colonies of this substrain on the plate were haemolytic and smooth.
In the protection test using the original strain the mice challenged by 670 LD50 survived more than the mice challenged by 42 LD50.
On the other hand, in the case of the substrain, there was observed no such result and the mice were killed to more extent with the increased dose of challenge.View full abstractDownload PDF (736K)
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