巻号一覧
20 巻, 11 号
選択された号の論文の9件中1～9を表示しています
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吉野 敏行1965 年 20 巻 11 号 p. 609-616
発行日: 1965/11/25
公開日: 2009/02/19
ジャーナル フリーMutual transfer of the fimbriation from the fimbriate donor cells to the non-fimbriate recipient cells was demonstrated in the selected recipient hybrids from the crosses between E. coli and Sh. flexneri.
In the recipient hybrids which have been transferred the ability of the fimbriation, there were shown some biological characteristics different from the parent recipient cells, from which the hybrids were originated, in addition to the alteration of the antigenic structure and the modification of sugar fermentation, which were transferred from the parent donor cells, the dissociation of the colony form was clearly observed.
Further observations on the segregations of the hybrids presented an evidence suggesting that the transfer of the ability of fimbriation might be due to the genetic recombination of fimbriation controlling gene(s), probably episome-like in nature, and that the recipient fimbriate hybrids seemed to be heterogenous in genotype.抄録全体を表示PDF形式でダウンロード (3888K) -
広尾 哲三1965 年 20 巻 11 号 p. 617-622
発行日: 1965/11/25
公開日: 2009/02/19
ジャーナル フリーAntibody promoting factor which flowed out into peripheral circulating blood during several hours in early stage after the intravenous invasion of antigen, in rabbits, was separated and identified with “inosine” by Dr. E. SAKAKIBARA et al.
In the present paper, the action of inosine was discussed about the net-production of bovine serum gamma globulin which was synthesized in the basic media, instead of measuring antibody which was formed in it during 2 hours after the start of liver slices incuvation. The net-production was measured by the quantitative precipitating method.
(1) Normal bovine liver slices formed 115±24.15ug/gr. w.w. of bovine serum gamma globulin as net-production, but when inosine was added to 2.5×10-4M as a final concentration into the basic media, it was increased about 2.5 times as compared with normal bovine liver slices.
(2) Several substances related to inosine, i.e. ATP, ADP, AMP, IMP, GMP, hypoxanthine, adenosine and adenine etc., were compared with the activating effect of inosine on the net-prodution of bovine serum gamma globulin. Adenosine displayed an increasing effect as well as inosine, ATP and ADP much less than adenosine, but on the contrary, hypoxanthine, adenine, AMP, IMP and GMP etc, had a little or no effect on the action.抄録全体を表示PDF形式でダウンロード (714K) -
第1報 菌の即時染色法石田 名香雄, 熊谷 勝男, 木村 瑞雄1965 年 20 巻 11 号 p. 623-626
発行日: 1965/11/25
公開日: 2009/02/19
ジャーナル フリーA basic dye commercially used as the brightener agent in detergents of the washings, , Calcofluor white, was found by Darken to be useful for the vital staining of several bacteria. Our study was further developed in respect to a specificity of a differential staining between animal and plant cells. Several cells of plant origin such as bacteria, Candida and Chlorophyta can be stained by the dye, but all of the tissue culture cells of animal origin so far examined can not be stained at all. When 100mcg/ml of the dye was added to the cells without receiving any fixation procedure, the plant cells can be differentiated from the animal cells, instantaeneously.
Although the living bacteria without any fixation are never stained with dyes such as methylene blue, the bacteria labeled with Calcofluor white, which were able to grow and divide, were stained with a solution of methylen blue.抄録全体を表示PDF形式でダウンロード (413K) -
一言 広1965 年 20 巻 11 号 p. 627-633
発行日: 1965/11/25
公開日: 2009/02/19
ジャーナル フリーA purified coagulase was obtained from a culture filtrate of Staphylococcus aureus St-213 which proved to be homogenous in immunological, electrophoretical and Spinco Model E ultracentrifugal studies. Treatment of the material with trichloroacetic acid prior to passing through Dowex-1 and Diethylaminoethyl-Sephadex A-50 columns yielded a product which showed a 280 fold increase in activity per mg of nitrogen than the crude culture filtrate. The sedimentation coefficient of the treated material was S20, w=1.59S.
The treated material was composed of 9 amino acids (i.e. lysin, aspartate, threonine, serine, glutamine, glycine, alanine, valine and methionine) and lacked carbohydrates, lipids and phosphates. Therefore, this purified material may be classified as a purified protein derivative (PPD) capable of exhibiting a specific coagulase type reaction against homologous and heterologous type antiserum when tested immunologically using the agar gel diffusion technique.抄録全体を表示PDF形式でダウンロード (3033K) -
西田 博1965 年 20 巻 11 号 p. 634-642
発行日: 1965/11/25
公開日: 2009/02/19
ジャーナル フリーNaturally occurring R factors are classified into two types, fi+ and fi-, depending on their fi characters. The term fi is an abbreviation of fertility inhibition and fi+ and fi- mean, respectively, the presence and absence of suppression of the functions of the sex factor F of Escherichia coli K-12.
It was found that fi- R factors reduce the efficiency of plating of phage λ in K-12; fi+ R factors did not have this inhibitory action. We have studied the mechanism of suppression of progeny formation of phage λ by fi- R factors and have obtained the following results.
1. The adsorption of phage λ was not altered by the presence of any R factors.
2. Transduction of the ability to ferment galactose with λdg was reduced by fi- R factors in the recipients.
3. The lysogenization with λ was also reduced by the presence of fi- R factors.
4. Spontaneous production as well as ultraviolet induction of λ was not affected by any R factors.
5. Zygotic induction of prophage λ was not affected by any R factors in the recipients.
6. “Helper effects” of modified normal λ were observed in the transduction of Gal+ with unmodified λdg into restricting E. coli carrying fi- R factors with the exception of R factor N-1.
From these results, it is concluded that suppression of progeny formation of phage λ by fi- R factors is due to some step after adsorption of the phage to the bacteria. It is further assumed that the nucleic acid of phage λ is broken down following injection.
Furthermore, it was found that phage λ undergoes host-induced modification in the host carrying some fi- R factors. At least two types of fi- R factors were recognized by the types of their restriction and host-induced modification of phage λ.
Lastly, CaCl2 exhibited an antagonistic action against the restriction of phage λ by fi- R factors; MgCl2 did not show such an antagonistic action.抄録全体を表示PDF形式でダウンロード (1059K) -
阿部 久夫1965 年 20 巻 11 号 p. 643-650
発行日: 1965/11/25
公開日: 2009/02/19
ジャーナル フリーUntil recently, Greig test has been used as the most important method of differentiation between Vibrio cholerae and Vibrio eltor. In these several years, however, it has become difficult to depend only on this method for the differentiation of these two groups, since there has been found many strains of V. eltor which are negative in soluble hemolysine test. Therefore, several other methods have been reported by various workers for the rapid differentiation of both groups, one of these is the difference of sensitivity against polymyxin-B. It was found by the authors that the same difference of sensitivity is also observed with colistin, an antibiotic closely related to polymyxin-B, and we have devised a disk method using disk which contains a proper amount of colistin. This report is chiefly concerned with the experimental conditions of the test method.
Results obtained are as follows:
1) V. cholerae and V. eltor can be definitely differentiated by the sensitivity against colistin.
2) Paper disks containing a proper amount os colistin can be used as the rapid and simple method for the differentiation of these two groups. V. cholerae forms a zone of inhibition of growth around the disk, whereas V. eltor forms no visible inhibitory zone.
3) For the differentiation test, the disk containing 1, 000u of colistin methansulfonate at pH 6.0 is most excellent; the disk which contains 150u of colistin sulfate is also useful. The 50u disk of polymyxin-B is inferior to the above mentioned colistin disks for this purpose.
4) Non-agglutinable vibrio showed the same response as V. eltor to colistin disk.抄録全体を表示PDF形式でダウンロード (860K) -
第1報 台湾におけるジフテリアの疫学および分離株の生物学的性状について郭 文芳1965 年 20 巻 11 号 p. 651-657
発行日: 1965/11/25
公開日: 2009/02/19
ジャーナル フリーA survey was made of the monthly incidence of diphtheria patients who visited the clinic of National Taiwan University Hospital, sites of diphtherial lesions and tracheotomy during two arbitrarily chosen periods, i.e. September, 1959-December, 1960 and June, 1963-January, 1965. At the same time, the numbers of specimens subjected to bacteriological examinations, and those bacteriologically confirmed were indicated for each month. In general, the peak of epidemic was from late autumn to early spring of the following year, (October, November, December and January), although sporadic cases were observed during interepidemic periods.
Among the 41 strains of C. diphtheriae isolated during the period, September, 1959-December, 1960, 78% belonged to intermedius type and the rest (22%) belonged to mitis type. In contrast, among 105 strains isolated during the period June, 1963-January, 1965, only 8.7% belonged to intermedius type, while 91.3% showed characteristics of mitis type. No gravis type strains were isolated. All of these strains showed sensitivity to penicillin, streptomycin, chlortetracycline, oxytetracycline, chloramphenicol, erythromycin, sigmamycin, ampicillin, and cloxacillin.
All the strains showed toxigenicity when tested by intradermal virulence test in rabbits, although there were differences in the grades of virulence among these strains. However, by Elek test 21% showed negative reaction. Among the strains which showed positive Elek test, those which required shorter time to produce bands of precipitin reaction tended to be more virulent in rabbit virulence test. There was no correlation between the types and virulence of these strains. Generally, there was no shift in the grades of virulence during these two periods of observation, although during September, 1959-December, 1960, more strains of intermedius type with greater virulence were isolated as compared with those strains of the same type isolated during the period June, 1963-Junuary, 1965 (0.05>P>0.01).抄録全体を表示PDF形式でダウンロード (860K) -
第2報 Candida albicans免疫処置によるウサギおよび他種動物における特異症候群の発現とその条件の検討岩田 和夫, 粟田口 重美1965 年 20 巻 11 号 p. 658-662
発行日: 1965/11/25
公開日: 2009/02/19
ジャーナル フリーConditions for establishing the unique syndrome indicating the involvement of the central nervous and vestibular systems of rabbits caused by Candida albicans was further traced.
Of the strains of C. albicans tested, those which were most virulent against mice proved to be most capable of inducing the syndrome in rabbits.
It was also found that other strains of the same species of Candida would stimulate a similar reaction.
Tests were made on other experimental animals. It was found that the Japanese quails and mice exhibited similar clinical symptoms, e.g., torticollis and ataxia, whereas chickens, guinea pigs and cats did not.抄録全体を表示PDF形式でダウンロード (3477K) -
1965 年 20 巻 11 号 p. 663-680
発行日: 1965/11/25
公開日: 2009/02/19
ジャーナル フリーPDF形式でダウンロード (2626K)
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