巻号一覧
22 巻, 5-6 号
選択された号の論文の6件中1～6を表示しています
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III. 種々の分画によるタンパクへのアミノ酸のとりこみ尾藤 行雄, 増井 武次1967 年 22 巻 5-6 号 p. 299-304
発行日: 1967/06/25
公開日: 2009/02/19
ジャーナル フリーAmino acid incorporation into proteins by various fractions of Pasteurella multocida was examined
and the following results were obtained.
1) Among the ribosomal fractions the components which migrated faster to the cathode in zone
electrophoresis were relatively active in amino acid incorporation, and the remainders were less active.
2) The incorporation of C14-leucine into proteins by ribosomes was found without the addition of
supernatant fluid. This reaction was not dependent upon the addition of ATP and ATP generators and
GTP, not prevented by ribonuclease or chloramphenicol, and proceeded at 0°C at the rate of about 60 per
cent of full activity. The majority of C14-leucine were incorporated into the inner part of peptide chains.
3) The incorporation of a small amount of C14-leucine into proteins was found by the addition of
supernatant fluid. From various observations it is suggested that this reaction was not the incorporation into
the internal linkage of peptide chains due to the synthesis of peptides.
4) The ribosomes had 68 S of sedimentation coefficient at the presence of 0.01M magnesium ion. In
contrast with the above finding, the ribosomes prepared in the absence of magnesium ion and dialyzed against
the buffer not containing magnesium ion were separated into two subunits, having a 25.6 S and 46.6 S of
sedimentation coefficient respectively. The treatment of ribosomes prepared in the absence of magnesium ion
with 0.1M EDTA led to the substantial disappearance of ribosomes and subunits.
5) The ribosomes were composed of 42.3 per cent of protein, 35.2 per cent of ribonucleic acid, 19.9
per cent of chloroform-methanol extractable substance and 0.6 per cent of polysaccharide.抄録全体を表示PDF形式でダウンロード (3543K) -
第1報 Micrococcus fasteus n. sp. Soeda et al. (1964)の性状について添田 百枝, 大友 道子, 青梅 美恵子1967 年 22 巻 5-6 号 p. 305-311
発行日: 1967/06/25
公開日: 2009/02/19
ジャーナル フリーMany cases of staphylococcal infection resistant to antibiotic therapy have been successfully treated by the use of toxoid prepared from resistant Staphylococcus aureus strains in our laboratory. In recent one year, we have encountered occasional cases with skin lesions which do not respond to such toxoid therapy in spite of the fact that morphologically staphylococci-like microorganisms can be isolated from the corresponding lesions almost in pure state.
We have collected 9 strains from patients with chronic, skin lesions which did not respond to both antibiotics and staphylococcal toxoid, and examined their detailed biological and serological properties in order to know the reason why they being resistant to such therapies.
These cocci are spherical cells similar to staphylococci, but they are usually arranged in dipro or tetra forms and never be grouped in irregular clusters as often seen in Staphylococcus aureus or epidermidis. In addition, they are not uniform in size and tend to be somewhat larger than staphylococci. For these reasons they are not so difficult to identify from staphylococci by simple microscopic examination of directstained smears from the lesions or their cultures.
Their biological properties are quite similar to those of staphylococci except for being incapable of producing nitrite from nitrate. They are variable in the coagulase test and almost all strains are resistant to the antibiotics, particularly to penicillin. Their colonies on agar media are also similar in appearance to those of staphylococci and cannot be identified by simple inspection.
A soluble, thermolabile exotoxin is produced by them which causes tissue necrosis or death in experimental animals and heamolyzes rabbit erythrocytes. This exotoxin is antigenic like staphylococcal exotoxin and gives rise to a specific antitoxin which neutralyzes it, and can be altered to toxoid form by treatment with formalin according to the method used for preparation of staphylococcal toxoid.
We have prepared M. fasteus toxoid adopting the representative strains and applied it to clinical cases for therapeutic purpose of chronic skin lesions uncontrollable by antibiotics or staphylococcal toxoid which proved capable of inducing complete disappearance of M. fasteus in a short period, which was followed by the rapid healing of the skin lesions.抄録全体を表示PDF形式でダウンロード (780K) -
清水 敏夫1967 年 22 巻 5-6 号 p. 312-320
発行日: 1967/06/25
公開日: 2009/02/19
ジャーナル フリーSawai et al. (1966) previously reported inactivation of venom of the habu (Trimeresurus flavoviridis) by dihydrothioctic acid (DHTA). In this study, effectiveness of DHTA in inactivating mamushi venom was investigated.The results showed that the hemorrhagic and necrotic action of mamushi venom were also prevented by DHTA which was added to the venom in equal or half amount and incubated at 37 degree C for one hour, and injected intramuscularly into the legs of white mice, subcutaneously into the plantar side of legs of mice or intramuscularly into rabbits.Mice and rabbits were then, immunized with mamushi venom treated with equal amount of DHTA. After four weeks, 3 boosters of the same amounts of toxoided venom were given at the interval of one week. And the neutralizing effects of the sera of immunized animals were tested. They were also challenged intramuscularly into the legs with the venoms. The results indicated that 0.1ml of sera of immunized animals neutralized from four to sixteen mhds (minimum hemorrhagic dose) by the method of intramuscular or intracutaneous injection.After the direct challenge of venom on immunized animals, it was also indicated that the local lesion of treated animals were decreased in considerable degree compared with control animals. Serum treatment of envenomated rabbits which were previously given toxoided venom were more successful than the animals without toxoid treatment.抄録全体を表示PDF形式でダウンロード (2313K) -
第3報 日本脳炎患者血清の2-Mercaptoethanol感受性について大塚 悟, 真子 憲治, 森 良一1967 年 22 巻 5-6 号 p. 321-325
発行日: 1967/06/25
公開日: 2009/02/19
ジャーナル フリーThe sensitivity of 2-mercaptoethanol (2-ME) on HI antibodies in patients with Japanese encephalitis were studied on 320 patients occurred in Fukuoka Prefecture during 1963-1966. The results obtained are as follows:
1. Most of the HI antibodies detected within 3 weeks after onset were found to be 2-ME sensitive, and therafter most of them changed to 2-ME resistant gradually.
2. Of 264 patients having detectable HI antibodies within 7 days after onset, 34 sera (12.8%) were found to be 2-ME resistant, and a rise of the titers were observed in 16 cases among them. Thus the findings indicate that, in some cases, HI antibodies may be insufficient to prevent JE virus infection.
3. Of 60 mortal cases having HI antibody, 55 (91.6 %) sera obtained within 7 days after onset were found to be 2-ME sensitive, and the remainning 5 2-ME resistant.抄録全体を表示PDF形式でダウンロード (558K) -
鈴木 ミツヱ, 原田 賢治, 松山 達夫1967 年 22 巻 5-6 号 p. 326-328
発行日: 1967/06/25
公開日: 2009/02/19
ジャーナル フリーThe authors performen the bacteriological and epidemiological observations on the food poisoning incidence in October, 1966, which was caused by a strain of E. coli.
Epidemiological observations revealed that the number of patients, with principal symptoms of stomachache, nausea, vomitting, diarrhea and fever, 15 with 18.5% of morbidity. the patients were all adult and the mean time of incubation period was 10.5h.
E. coli strains were isolated almost in pure culture on the media used diagnostically from 5 patients examined. These cultures originated as single lactose-fermenting colonies on the media, which were there-after subcultured and identified by fermentative and biochemical reactions as Escherichia coli. These cultures were also identified by biological and serological reactions as E. coil O-111, B4 and agreed with the biological properties of type 1 described by F. Kauffmann. Widal reaction did not show any rise in O- antibody against the strain E. coil O-111, B4 which was isolated from the patients.抄録全体を表示PDF形式でダウンロード (369K) -
松山 達夫, 鈴木 ミツヱ, 中嶋 隆, 原田 賢治1967 年 22 巻 5-6 号 p. 329-331
発行日: 1967/06/25
公開日: 2009/02/19
ジャーナル フリーThe authors performed the bacteriological and epidemiological observations on the food poisoning incidence in June, 1965, which was caused by a food “sushi”.
Epidemiological observations have revealed that the number of patients with principal symptoms of gastroenteritis without fever were 66 with 61.1 per cent of morbidity. The mean time of incubation period was 3-4h.
The strain of Staphylococcus aureus was isolated from the food caused the poisoning, and also from stools and vomits of patients. They were identified by fermentative and biochemical reactions as Staphylococcus aureus, of phage type 85/80抄録全体を表示PDF形式でダウンロード (372K)
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