巻号一覧
22 巻, 8 号
選択された号の論文の7件中1～7を表示しています
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I) 芽胞形成過程におけるジピコリン酸の細胞内分布の変化川崎 近太郎, 近藤 雅臣, 西原 力1967 年 22 巻 8 号 p. 463-467
発行日: 1967/08/25
公開日: 2009/02/19
ジャーナル フリーIntracellular distribution of dipicolinic acid (DPA) during sporulation was studied by using a strain of B. subtilis. In the initial stage of sporulation, DPA was detectable only in the soluble fraction and the content rapidly decreased with increase in the content of DPA in the residue fraction. In contrast, no DPA was detectable in the supernatant derived from the cells in the stage of maximal sporulation.
When the matured spores were mechanically disrupted for a relatively short time, it was found that DPA could easily be released from the matured spores and the kinetic pattern of the release was not parallel with the release of protein and nucleic acid from the spores. On the distribution of DPA in the disintegrated spores, it was also found that this compound is mostly detected in the residual supernatant after ammonium sulfate fractionation.
From these results, it was suggested that DPA may be mainly synthesized outside the forespore and then accumulated inside the spore coat in a free from or at least in a loosely bound form.抄録全体を表示PDF形式でダウンロード (583K) -
川崎 近太郎, 近藤 雅臣, 手島 邦和1967 年 22 巻 8 号 p. 468-471
発行日: 1967/08/25
公開日: 2009/02/19
ジャーナル フリーForespores of B. subtilis were isolated from the sporangium by lysozyme treatment and the biological nature was compared with those of vegetative cells and mature spores. Isolated forespores could form colonies on nutrient agar plate. The forespores were resistant to the action of lysozyme and antibiotics but they were not resistant to heat treatment at 80C for 30min. UV killing curve of forespores showed the different pattern from those of vegetative cells and mature spores.抄録全体を表示PDF形式でダウンロード (421K) -
木下 恒雄, 中村 昌弘1967 年 22 巻 8 号 p. 472-477
発行日: 1967/08/25
公開日: 2009/02/19
ジャーナル フリーIt might be presumed that one of the possible etiologies of diarrhoe caused by non-pathogenic bacterial substance would be that some kind of enterobacteria of bacterial flora in intestinal tract absorbs VB12 which is necessary for digestion, prior to combination with intrinsic factor. Therefore, absorption conditions of VB12 to the bacterial cells were studied in order to clarify a possible etiology of diarrhoe.
Several factors affecting absorption of vitamin B12 by Lactobacillus leichmannii ATCC 4797 which requires VB12 for growth were determined by radioactive measurement, comparing to VB12 non-required other lactobacilli. Whole cells and cell wall fraction were used throughout the studies. The results obtained were as follows.
1. VB12 were absorbed specifically to the whole cell and cell wall of L. leichmannii for very short time and at low temperature, however, there was no complete relationship of absorption rate between dosage of vitamin B12 and numbers of bacterial cells.
2. Properties of VB12 absorption by cell wall were somewhat different from that by whole cells. These differences might be due to different characteristic of inner side of cell wall from out side of the cell.
3. Absorption of VB12 by the bacteria were inhibited by heating at 100°C, by treatment with formalin, ethanol and chloroform, and by digestion with trypsin, lipase as well as amylase, but were markedly stimulated by digestion with papain.
4. VB12 absorption to the cell wall was significantly stimulated in the presence of salts, whereas there were no effects of salts in the cases of whole cells.
5. No specific absorption of VB12 by VB12 non-required strain of lactobacilli occurred, even if the bacilli were administered by various treatments which might possibly convert from VB12 insusceptible cells to susceptible ones.抄録全体を表示PDF形式でダウンロード (723K) -
原田 賢治, 重原 進, 亀田 三男, 松山 達夫1967 年 22 巻 8 号 p. 478-481
発行日: 1967/08/25
公開日: 2009/02/19
ジャーナル フリーIn june, 1966, there was an outbreak of a water-borne epidemic of bacillary dysentery, in 4 areas of a city in Gunma Prefecture. The prevalence began on June 22 and came to an end on July 9, and 455 patients were detected. All of the isolated strains from patients were identified as Shigella sonnei phase 1, Col type 6 and 92.4% of which were tetracycline (TC) factors.
The four epidemic areas were supplied with two systems of public water derived from two wells independently. The two wells were located closely, 29m apart from each other. The wells were contaminated by sewage whic had leaked out from a sewer pipe, and this was considered as the cause of water-borne bacillary dysentery.
The water from the supplying system which was suggested to be contaminated, was collected out at various time, and was examined bacteriologically. In the cause of sampling, it was able to isolated two cultures of Shigella sonnei phase 1, Col type 6 with R (TC) factor from 100ml of a sample which was collected 5 days before the outbreak. From other samples were detected Escherichia coli but not dysenteric bacilli.
In the previous records of outbreak of water-borne disease, their nature and causation are not always clear. And the causative bacteria could not be confirmed directly from the comtaminated water.
As is apparent in this report, the authers were able to detect Shigella sonnei from the water supply which was the cause of water-borne bacillary dysentery, and proved that the characters of isolated strains from patients and that from water were identical.抄録全体を表示PDF形式でダウンロード (501K) -
その1. 本邦における野性株による成績松尾 吉恭, 名草 芳博1967 年 22 巻 8 号 p. 482-489
発行日: 1967/08/25
公開日: 2009/02/19
ジャーナル フリーThe authors observed a limited multiplication of acid-fast bacilli in mouse foot-pads inoculated with Mycobacterium leprae from an untreated patient with lepromatous leprosy in this country. There was no evidence of bacillary increase in any of the mice inoculated with a heat-killed suspension. Nor were any macroscopically visible changes noted. The acid-fast bacilli in the inoculum and the harvest were transferred to Ogawa's egg media, and incubated at 33°C and 37°C. The cultures were negative, eliminating the possibility of superinfection by culturable mycobacteria. Subcutaneous inoculations of mice in the abdomen did not produce any lesions, indicating that Mycobacterium lepraemurium, a known unculturable mycobacterium, did not exist in the inoculum and the harvest. There was an arrest in the multiplication of the bacilli at about 105 to 106 per foot-pad. These findings were compatible with those of Shepard and others, who recently reported degrees of successful transmission of leprosy to mice. It is likely that the authors isolated a strain of M. leprae in mouse foot-pads. The viability of M. leprae was not affected by freezing and storing at -30°C for 18 days.抄録全体を表示PDF形式でダウンロード (1113K) -
第1篇 緒言ならびに増菌法について吉井 善作, 原田 肥育, 坪田 洋子, 佐々木 正人, 三浦 伝一, 世良 行男, 二五田 公俊1967 年 22 巻 8 号 p. 490-499
発行日: 1967/08/25
公開日: 2009/02/19
ジャーナル フリー緒言においてはAn菌の分類学的研究が充分に確立されていない現状を述べた。そしてAn菌検索法の目的と内容を概述すると共に,従来の方法が必ずしも能率的でなかつた点を指摘し,簡易化を目指した動機を記した。
増菌培養法においては培地と培養条件を決定し,材料とその処理法についての実験を行ない,増菌法の必要性と使用区分を明らかにした。酵素学的術技は別に報告しているのでここでは概略を簡記するにとどめた。
以上の内容を要約再録すると次のごとくである。
(1). 検体(特に糞便の場合)が少数例の際は生食水で洗浄後,培地へ注入する。多数例の場合は洗浄せず,0.5g宛培地へ投入,三次にわたる継代培養を行なう。
(2). 材料の加熱処理は従来法の80°C, 20分を踏襲したが,熱耐性の弱い種の存在を予想すると将来は60°C, 20分なる条件も考慮せねばなるまい。
(3). 培地はBrewer's mediumに若干の変法を行なつたものに決定した(A培地)。これは好気,嫌気培養の兼用が可能であり,増菌効果はブイヨン,肝々ブイヨンの両者に劣らないのみならず,Anテストにわれわれの提唱する簡易法の適用が可能である。なお,好気培養専用(B培地),嫌気培養専用(A-1培地)をもそれぞれ考案した。
(4). 培養条件は37°C, 4日間に統一した。
(5). 増菌効果の推定にはAn活性を調べることによつて行なつた。このAnテストは試料洗浄の場合は第一次増菌培養時(第二次増菌培養は予備のため),非洗浄の場合は第二次増菌培養時行ない,予備に第三次増菌を補足することにした。
(6). 増菌法はAn菌検索法においては効果的であることを立証し,その必要性を強調した。但し,雑菌による汚染スタムの純化には必ずしも必要ではない。
(7). われわれの増菌法は従来法に比し,術技体系において著しく簡便であり,精度においても劣らないことを立証した。抄録全体を表示PDF形式でダウンロード (1334K) -
柏崎 守, 波岡 茂郎, 赤池 洋二1967 年 22 巻 8 号 p. 500-504
発行日: 1967/08/25
公開日: 2009/02/19
ジャーナル フリー無菌豚に乳酸菌を先行定着させ,遅れて大腸菌を投与した場合,両者の菌数が腸管内でどのように変化するかをしらべたところ,つぎのような成績が得られた。
1. 7日令の無菌豚に豚糞便由来の乳酸菌を径口投与したところ,糞便中への該菌の出現は,24時間後では認められなかつたが,48時間後には糞便1g中109個程度が検出されるようになり,以後この菌数は安定して持続した。
2. 投与乳酸菌が,糞便中109個程度に先行定着した5日後に,豚に病原性のない大腸菌を追加径口投与したところ,該菌の増殖が乳酸菌で抑制されることなく,すでに24時間後の糞便中には一挙に1010個程度検出され,大腸菌優位の糞便菌叢が成立した。この菌叢は,48時間後でも変わらなかつた。
3. 乳酸菌および大腸菌がそれぞれ定着した消化管各部での両菌の均衡は,上部消化管では乳酸菌が,また下部消化管では大腸菌がそれぞれ優位であつた。
4. これらの豚は,臨床上健康状態を保持し,下痢などの発生もみられなつた。抄録全体を表示PDF形式でダウンロード (695K)
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