Based on the previous finding that the Crepidotus polysaccharide exhibited a definite suppressive effect against Sarcoma 37 in mice, the effective dose, the antiumor spectrum and the route of administration were examined in this report. It was found that the injection of 250∼50mg/kg suppressed effectively the tumor in most of animals. Both pre- and post-treatment with the polysaccharide were effective. As to the route of injection, the intraperitoneal administration was most effective. The subcutaneous injection gave the least effect. When the dose was divided, 3 injections of 10μg gave high inhibition rate. While the polysaccharide suppressed the solid tumor of Ehrlich carcinoma and Sarcoma 180 in addition to Sarcoma 37, it failed to inhibit ascites form of these tumors and AH 13 ascites hepatoma in the rat. Also, a definite inhibition was not seen with syngeneic tumor such as MH 134 in C3H/He and solid tumor recently derived from spontaneous mammary carclnoma. Thus, the antitumor action of the polysaccharide showed a similar pattern to that of the yeast cell wall polysaccharide.
1. By the procedure of transferring cultures to medium containing 50% of ox bile, the bile-tolerant strains of Lactobacillus and Streptococcus could be made after 15th passage. 2. The variants were more tolerant to sodium cholate, sodium taurocholate, sodium glycocholate and sodium desoxycholate, respectively, than their parent strains. 3. It was considered that the variants were more tolerant to bile salts of human being than their parent strains.
Four strains of mouse-passage M. leprae provided by Shepard were inoculated into the left hind foot-pads of CF#1 mice. The number of bacteria inoculated per foot-pad ranged from 7.89×102 to 2.37×103, 8.6 to 20.6% of which were stained solidly. The mice were housed in an animal room at 20±1°C, and fed the usual pelleted commercial chow. Three to four mice from a group of mice which were inoculated with a strain were sacrificed about 6 and 9 months after inoculation, and the inoculated foot-pads were dissected off and pooled for each harvest. The number of acid-fast bacteria was counted, the ratio of solidly staining bacteria determined and the generation time calculated. It was conclusively demonstrated that M. leprae could be transmitted to the mouse foot-pad. Multiplication of M. leprae was consistent but limited so that it reached a maximum logarithmic phase by about 6 months after inoculation, which was followed by a plateau phase. The calculated generation time from inoculation to a maximum logarithmic phase ranged from 18.2 to 23.6 days. The ratio of solidly staining bacteria rose during this period and declined later. The acid-fast bacteria from inoculum and harvest were inoculated to Ogawa's medium and incubated at 33° and 37°C for 3 months. No cultivable mycobacteria were isolated. Subcutaneous inoculation of the bacteria to mice failed to produce any lesions.