巻号一覧
24 巻, 11 号
選択された号の論文の7件中1～7を表示しています
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II. トキソイド製剤中の培地成分赤真 清人, 山本 昭夫1969 年 24 巻 11 号 p. 595-601
発行日: 1969/11/25
公開日: 2009/02/19
ジャーナル フリーIn this series of studies, peptone and heart infusion were studied for allergenicity with special reference to the reaction of immediate type in guinea pigs, since some of the side reactions of tetanus toxoid following the inoculation were apparently related to the allergy caused by materials contained in the medium used for the toxin production.
The previous paper showed that heart infusion was more allergenic than peptone, and that the allergenicity was presumably related to substance(s) of large molecular weight. On the other hand, the toxin production was promoted by substance(s) of small molecular weight contained in the infusion.
This paper demonstrated the existence of allergenic substance(s) derived from the constituents of the medium in the preparations of purified tetanus toxoid.
To remove the antigenic substance(s) effectively, fractionation with ethanol or dialysation of peptone or heart infusion was tried, and the following conclusions were obtained.
1) All the components effective for the toxin production in heart infusion were dialyzable. A maximum toxin production was shown with an amount of 0.5% of the dialysate. Most of the effective materials of peptones were also dialyzable.
2) No significant differences were observed in the potencies of the toxins between media consisting of dialyzed and undialyzed materials of both constituents.
3) The toxoid sample derived from a medium consisting of the dialysate of both components was higher in purity and lower in allergenicity to materials in medium than that derived from a medium containing undialyzable materials. No significant difference was found in potency between both toxoid samples.
4) The effective components for toxin production in heart infusion were soluble in 85% ethanol. The soluble fraction, however, still contained allergenic materials, though it induced no precipitation with trichloroacetic acid.抄録全体を表示PDF形式でダウンロード (836K) -
小林 卓1969 年 24 巻 11 号 p. 602-611
発行日: 1969/11/25
公開日: 2009/02/19
ジャーナル フリー1. Antibody response hardly occurred for about 3 months in mice administered with 50μg per g of body weight of capsular polysaccharide of Klebsiella pneumoniae (CPS), while mice responded well when administered with 0.15μg per g of body weight of CPS.
2. Immune response of mice paralyzed by CPS to such unrelated antigens as influenza virus A (PR 8), sheep red blood cells, and flagellar antigen of Salmonella typhosa was partially inhibited.
3. Cold agglutinin to chicken red blood cells was produced in mice paralyzed by CPS. There was a close correlation between the production of cold hemagglutinin and the dose of CPS administered.
4. No blockade of the reticuloendothelial system by India ink affected significantly the immunsuppression of paralyzed mice to the unrelated antigens.
5. Mice paralyzed by CPS showed a marked increace in γ-globulin of the serum and severe hypertrophy of the spleen accompanied by an increase in plasma cells and Russell bodies.抄録全体を表示PDF形式でダウンロード (1414K) -
服部 宏1969 年 24 巻 11 号 p. 612-622
発行日: 1969/11/25
公開日: 2009/02/19
ジャーナル フリーBy the enzyme-labeled antibody method, Escherichia coli was examined for cellular localization of O antigen. Peroxidase was conjugated with γ-globulin which had been isolated from anti-O rabbit serum. Coupling was accomplished, by using p, p'-difluoro m, m'-dinitrophenylsulfone (FNPS).
Conjugated antibody reacted with Escherichia coli cells or cell wall preparation. The electron microscope was used for observation of the reaction. Electron density increased in places unequally and mosaic-like in the three most external layers of the cell wall. This observation is interesting, since Petris has suggested that lipopolysaccharide may make mosaic with lipoprotein in the cell wall of Escherichia coli. When the conjugate reacted with heterogeneous Escherichia coli cells, there was no increase in electron density. The peroxidase-labeled antibody method seems to be excellent, as it has a high specificity and conjugate penetrates tissues more easily than ferritin.
In addition, the ferritin-labeled antibody method, the cell fractionation method, and the Ouchterlony method were used to observe the cellular localization of O antigen.
Endotoxin was extracted by the phenol-water method or with trichloracetic acid. When observed by electron microscopy, endotoxin showed a variety of shape and had an extremely large myelin-like structure. The section of endotoxin appeared as three layers, and had some similarities to the outer surface of the whole cell.抄録全体を表示PDF形式でダウンロード (7757K) -
秋元 佑介1969 年 24 巻 11 号 p. 623-630
発行日: 1969/11/25
公開日: 2009/02/19
ジャーナル フリーPaying attention to the ontogenetic dissociation of immunologic competence in chickens, i.e. the thymus-dependent and the bursa dependent immune systems, the author performed some experiments in an attempt to elucidate the complicated immunopathogenetic mechanism of expeimental allergic encephalomyelitis (EAE). Seven-week-old chickens were divided into five groups, which were intact normal, surgically bursectomized, hormonally bursectomized, surgically thymectomized, and given rabbit anti-thymic lymphocyte serum after surgical thymectomy, respectively. All of them were sensitized with encephalitogenic basic protein (EBP) in Freund's adjuvant. EBP had been extracted from the bovine spinal cord by Kies's method.
The “bursaless” chickens lacking in humoral immunity exhibited clinical symptoms, such as ataxia and paralysis, and typical demyelinative lesions in the brain and spinal cord, showing the complete absence of anti-EBP antibody in serum with certainty. By contrast, the “thymusless” chickens devoid of cellular immunity manifested no clinical or histological evidence of EAE, in spite of the presence of serum anti-EBP antibody. It was histologically demonstrated that EAE of a “bursaless” chicken was transferred passively to another “bursaless” bird by the aid of sensitized lymphoid cells, but not to a “thymobursaless” bird. These results suggest that the thymus-dependent lymphoid cells of recipients passively sensitized with the donor's sensitized cells may participate in the development of EAE.
From the findings mentioned above, it was apparantly confirmed that EAE could be provoked by sensitized thymus-dependent lymphoid cells without participation of humoral anti-EBP antibody. Accordingly, the demyelination of EAE may be attributed to the glio- and myelinotoxicity of cellular antibody to EBP.抄録全体を表示PDF形式でダウンロード (2413K) -
第1報 colicin産生の基礎的条件久保田 憲太郎, 石倉 康宏, 和田 英太郎, 刑部 陽宅1969 年 24 巻 11 号 p. 631-637
発行日: 1969/11/25
公開日: 2009/02/19
ジャーナル フリーSince Abbott and Shannon introduced a systematic colicin typing of Shigella sonnei their method have widely been used for epidemiological studies of group D Shigellosis. However, the inhibition pattern based on their qualitative determination somewhat lacks in reproducibility.
As the first step to improve the colicin typing of Shigella sonnei, attempts were made to establish a quantitative method for the determination of colicin activity and then examine basic conditions for the colicin production quantitatively. Types 5, 11, and 12 strains were used throughout the experiment. Both liquid and solid media were employed.
The following results were obtained.
1. Dorset egg medium was superior to cooked meat medium for the storage of colicin-producing strains.
2. Colicin production was better at 37°C than at 30°C, although the difference in bacterial growth was little between the two temperatures of incubation.
3. Similar to Escherichia coli which produces K and V colicins, yeast extract or cattle blood increased the colicin production by Shigella sonnei. Hence brain heart infusion (Difco) supplemented with 5% cattle blood was a medium suitable for the colicin production.
4. Basic conditions for the colicin production on the solid medium were similar to those in the liquid medium, except that the colicin activity of type 12 strain began to decline at 48 hours of cultivation.抄録全体を表示PDF形式でダウンロード (783K) -
第2報 定量法によるAbbott & Shannon法の再検討石倉 康宏, 児玉 博英, 刑部 陽宅, 久保田 憲太郎1969 年 24 巻 11 号 p. 638-643
発行日: 1969/11/25
公開日: 2009/02/19
ジャーナル フリーThe quantitative method described by the authors in their previous report was used to examine the production of colicin from various type strains of Shigella sonnei, and the specificity and susceptibility of the indicator strains.
The results obtained are summarized as follows.
1. There were quantitative differences in the production of colicin as determined by Abbott and Shannon's method amoung the type strains used. A significant difference in the production of each colicin was observed in case a strain produced two different types of colicin.
2. Some indicator strains used by Abbott and Shannon exhibited instability in their susceptibility to the same colicin produced by various strains to be typed, depending on the amount of colicin, or on the degree of the colicin activity.
This may be one of the main causes for the occurrence of unstable patterns classified on the basis of Abbott and Shannon's typing. Poor reproducibility observed in certain cases may also be improved, if the bacterial counts of the indicator strains are adequate.抄録全体を表示PDF形式でダウンロード (631K) -
1969 年 24 巻 11 号 p. 644-655
発行日: 1969/11/25
公開日: 2009/02/19
ジャーナル フリーPDF形式でダウンロード (1604K)
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