巻号一覧
24 巻, 3-4 号
選択された号の論文の6件中1～6を表示しています
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選択毒性を中心として田中 信男1969 年 24 巻 3-4 号 p. 169-173
発行日: 1969/04/25
公開日: 2009/02/19
ジャーナル フリーPDF形式でダウンロード (635K) -
1. O2:K3およびO2:K28K抗原の免疫学的特異性とその糖構成工藤 泰雄1969 年 24 巻 3-4 号 p. 174-182
発行日: 1969/04/25
公開日: 2009/02/19
ジャーナル フリーStudies were made to clarify the immunochemical properties and sugar constituents of K antigens of Vibro parahaiemolyticus. Two strains belonging to the O group 2, the Nakatsukawa (O 2:K 3) and the T-1698 (O 2:K 28), were employed. Purification was made by means of phenol-water extraction, several times of freezing and thawing to remove nucleic acid and ultracentrifugation.
The properties of the K substances finally obtained were as follows.
1. K substances showed high degrees of serological specificity when they were subjected to reaction with homologous anti-K sera by the Ouchterlony gel-diffusion method.
2. Contamination with O antigen or any other antigenic substance in these final products were denied by the Ouchterlony method, although the K substances gave two specific precipitation lines.
3. These two K substances were considered to be a kind of hapten. When rabbits were injectedwith 80mg of the substance, no detectable K antibody was produced in them. Besides, the substances showed no toxic effect on mice.
4. Chemical analyses of these substances revealed that each substance was an acidic polysaccharide which consisted mainly of galactose and rhamnose, and that it contained no trace of such acidic sugars as uronic acid and sialic acid, in spite of the acidic characteristics it exhibited.抄録全体を表示PDF形式でダウンロード (6607K) -
DNaseおよびCoagulaseの有無と病原性の関係上條 清明1969 年 24 巻 3-4 号 p. 183-188
発行日: 1969/04/25
公開日: 2009/02/19
ジャーナル フリーStudies were carried out on five staphylococcal strains with special reference to coagulase production, deoxyribonuclease activity and virulence. The experimental animals used were mice of the dd strain. They were inoculated intraperitoneally throughout the experiments.
Results obtained are as follows.
1) The lethal effect of the broth culture differed from strain to strain, irrespective of coagulase production and nuclease activity. Some of the nuclease-positive strains were of lower virulence, while some of the coagulase-negative strains showed a high virulence.
2) The same result was obtained in any cases, as determined by lethality for mice within 14 days after inoculation or by the time of death after inoculation. It was not necessary to observe the animals beyond 72 hours for this purpose.
3) Some strains produced a toxic substance in the culture filtrate. The substance was eluted from an ECTEOLA- or DEAE-cellulose column with 0.001M∼0.02M phosphate buffer (pH 7.1). In some strains which were coagulase-negative and nuclease-positive, the culture filtrate showed an inhibiting effect against the virulence of the other strains.
4) Cell infiltration, induration and abscess formation were seen at the site of injection in some cases injected with coagulase-positive, nuclease-positive strains. Any coagulase-negative, nuclease-positive strains did not induced such effect, even though they showed a relatively high virulence.
5) The inoculated organisms were distributed mainly in liver, spleen, and kidney, irrespective of virulence.抄録全体を表示PDF形式でダウンロード (873K) -
佐藤 成美, 川田 十三夫1969 年 24 巻 3-4 号 p. 189-194
発行日: 1969/04/25
公開日: 2009/02/19
ジャーナル フリーA substance stimulating contraction of the isolated rat uterus in Tyrode solution was found both in the culture supernatant and in the soluble cellular fractions of Clostridium botulinum type A by the Magnus method. The contractile substance was separated from the culture supernatant and partially purified by Dowex column chromatography and paper chromatography.
This substance had a molecular weight lower than 180 and gave a pink spot (Rf value, 0.13) on the paper chromatogram run in butanol: acetic acid: water (3:1:1) by ninhydrin. It exhibited no positive reaction with diazo, Dragendorff, or Sakaguchi reagent.抄録全体を表示PDF形式でダウンロード (620K) -
室橋 豊穂, 吉田 幸之助1969 年 24 巻 3-4 号 p. 195-201
発行日: 1969/04/25
公開日: 2009/02/19
ジャーナル フリーA biological significance was suggested to be shown by an acid-fastness map devised from investigation of the effect of ultraviolet irradiation on the acid-fastness of mycobacteria. Then, attempts were made to cultivate Mycobacterium lepraemurium Hawaii from nodules of infected mice in a cell-free, semi-synthetic soft agar medium. The basic composition of this medium was modified to some extent from that used for the successul cultivatioh of M. paratuberculosis Johnei.
Experiments were carried out repeatedly five times. Those organisms which had multiplied even to a limited extent were observed to form microcolony-like masses, which were composed of elongated, strongly acid-fast rods. It was interesting to note that when a medium contained both glycerol and glucose as carbon sources in addition to ferricion, it seemed to support the growth of M. lepraemurium to some extent.抄録全体を表示PDF形式でダウンロード (6982K) -
I. 患者結節からの人癩菌分離培養の最初の試み室橋 豊穂, 吉田 幸之助1969 年 24 巻 3-4 号 p. 202-211
発行日: 1969/04/25
公開日: 2009/02/19
ジャーナル フリーOne-tenth ml each of bacterial suspensions derived from nodules of 4 different L-type patients was inoculated into 0.1% semiliquid agar media of various semi-synthetic base compositions and incubated at 37°C. The results revealed that rather large colony-like masses of bacteria had appeared, in most cases, after about 20 weeks and persisted even after about one year of incubation at 37°C. These masses varied in size and shape. Generally, they were composed of smaller, round bacterial masses of 10 to 20μ in diameter, confluent with one another. Each mass seemed to have boundary around it. It consisted of strongly acid-fast rods, stainable with malachite green. It was suggested that the bacterial masses might be colonial growths.
Bacterial suspensions prepared from nearly one-year-old cultures by centrifugation induced essentially the same intradermal reaction in leprous patients as Dharmendra's antigen. Therefore, it was revealed clearly that the bacterial masses appearing in culture tubes were composed of M. leprae.抄録全体を表示PDF形式でダウンロード (7969K)
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