巻号一覧
24 巻, 7 号
選択された号の論文の6件中1～6を表示しています
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武井 盈, 深津 利雄, 戸松 功克1969 年 24 巻 7 号 p. 311-316
発行日: 1969/07/25
公開日: 2009/02/19
ジャーナル フリーStrain ATCC 9811 of Streptococcus mitis, which has been suggested to belong to Streptococcus sanguis by a recent worker, was subjected to continuous culture in various types of sugar broth at 37°C every 3 days.
Culture was performed in a glass vessel (30ml in culture volume) at a flow rate of 18∼20ml per hour. The culture broth was agitated by a magnetic stirrer (at 3∼4 rps). One percent of such sugar as glucose, fructose, galactose, sucrose, or raffinose, and mixtures of monosaccharides were added to a basic broth which was composed of peptone, yeast extract and sodium chloride.
Bacterial plaques were formed on the surface of the glass vessel in the early stage only when culture was made with sucrose broth. They were peeled by a rotating rod of the agitator. As they grew larger, flow-out cells decreased in number, and the pH of the culture broth remained at a low level, reaching the lowest value in sucrose broth. Bacterial plaques were also formed in such types of broth containing 0.5, 0.2 and 0.1% of sucrose. There was a decrease in pH value after plaque formation. When culture was performed in sugar-free broth with an intermittent addition of sucrose up to approximately 1.0% (3 times per day at intervals of 4 hours only in daytime; there was no addition at night), bacterial plaques were also formed and the number of flow-out cells fluctuated irregulary. The pH value was maintained at a low level even after the interruption of sucrose supply at night.抄録全体を表示PDF形式でダウンロード (786K) -
上田 益巳1969 年 24 巻 7 号 p. 317-324
発行日: 1969/07/25
公開日: 2009/02/19
ジャーナル フリーSubcellular fractions were prepared from cells of Candida albicans and Candida tropicalis by disintegrating them with a French press type apparatus and successive fractional centrifugation. As a result, a cell-wall, a particulate, and a supernatant fraction were obtained. In analyses of these fractions by reducing with dithionite minus oxygenated difference spectra, it was shown that almost all the cytochromes, which were composed of a, b and c, or c plus c1, were distributed in the particulate fraction. Only a small amount of cytochrome was found in the cell-wall fraction and a trace amount of cytochrome c seemed to be distributed in the supernatant fraction. Furthermore, the greater portions of the succiante-and NADH-cytochrome c reductase activities were distributed in the particulate fraction, and small amounts of them in the other fractions. The particulate fraction has already been known to be composed mainly of the intracytoplasmic membrane system.
Subcellular fractions of another kind were prepared from sonically disintegrated spheroplast preparations of the cells of both organisms by successive fractional centrifugation. The fractions obtained in this manner were called a mitochondrial, a small particulate, and a soluble fraction. Electron microscopic observation of thin sections of these fractions revealed that the mitochondrial fraction consisted relatively homogeneously of mitochondria, and that the small particulate fraction was composed mainly of small fragments of the outer membrane of mitochondria, vacuoles originated from their inner membrane and other intracytoplasmic membrane systems. In investigation of the distribution of cytochromes in these fractions, it was proved that almost all the cytochrome systems that had been observed mainly in the particulate fraction were now distributed to a similar extent both in the mitochondrial and in the small particulate fractions, and that only a trace amount of cytochrome c was found in the soluble fraction. On the other hand, the succinate-and NADH-cytochrome c reductase activities were the most abundant in the mitochondrial fraction, relatively scanty in the small particulate fraction, and hardly observable in the soluble fraction.抄録全体を表示PDF形式でダウンロード (2816K) -
影山 成章1969 年 24 巻 7 号 p. 325-330
発行日: 1969/07/25
公開日: 2009/02/19
ジャーナル フリーThis investigation was designed to elucidate possible variation in the degree of natural resistance of mice which might arise as a consequence of experimental Salmonella infection, and to predetermine, if possible, the susceptibility of an individual mouse to Salmonella.
The capillary-tube insertion method which was developed in this investigation was carried out in the following manner.
One-tenth volume of broth culture (18∼24 hours) of a definite strain of bacteria was mixed with 0.8% nutrient agar previously melted and kept at 47°C. A capillary tube 0.75mm in inside diameter and 4mm in length was filled with this mixture, and then inserted into the subcutis of the abdominal wall of a mouse for 48 hours. After that the number of surviving bacteria in the tube was counted by the plate method.
Actually, during the insertion period of 2 days, humoral factors diffused well, and phagocytes infiltrated moderately in the tube. The number of viable bacteria seemed to represent the sum of humoral and cellular resistance. It is conceived that this method has many advantages for studies on the natural resistance of experimental animals.
When three capillary tubes containing S. enteritidis 1891, Escherichia coli B, and Staphylococcus aureus 209 P, respectively, were inserted into individual mice, the number of surviving bacteria of each strain in the same mouse was inversely proportional to the degree of the virulence of such strain. There existed a linear relationship between the number of viable organisms and the degree of natural resistance of each mouse. In other words, a mouse resistant to S. enteritidis was also resistant to E. coli B and Staph. aureus. Reversely, a mouse susceptible to the former was susceptible to the latter. A parallel relationship was demonstrated between the survival number in the tube inserted into the subcutis and the abdominal cavity.
Accordingly, it was assumed that the susceptibility of each mouse to S. enteritidis 1891 might be predetermined from the viable number of Staph. aureus in a tube inserted in the subcutis.抄録全体を表示PDF形式でダウンロード (3377K) -
2. K1∼46各K抗原の免疫学的特異性とその糖構成工藤 泰雄1969 年 24 巻 7 号 p. 331-337
発行日: 1969/07/25
公開日: 2009/02/19
ジャーナル フリーIn the previous paper, it was reported that the purified K antigens of Vibrio parahaemolyticus strains O2:K3 and O2:K28 had been shown to have antigenic specificity by the Ouchterlony method, and that analysis had clarified that these antigens were mainly composed of acidic polysaccharide. The present paper deals with the immunochemical characteristics of the remaining 43 of the 46 types of purified K antigens of the organism.
The results obtained are summarized as follows.
1) All the K antigens, except K 27 and K 16 which failed to release any K antigenic substance by the phenol water extraction method, were proved to be substances mainly composed of acidic polysaccharide.
2) Thirty-nine of the 43 types of purified K antigens showed antigenic specificity against homologous anti-KO serum by the Ouchterlony gel-diffusion method.
3) It was proved serologically that O3:K30 and O5:K14 had exactly the same K antigen, although they had different O antigens.
4) The pilot strain of the organism, O11:K35, which has lately been added as a new serotype, gave completely the same results with the pre-existing O5:K15 pilot strain.
5) Chemical analysis of the purified K antigens revealed that the sugar composition varied with the type of K antigens, except O2:K3 and O2:K28. The sugar detected were glucose, galactose, mannose, fucose, rhamnose, ribose, glucosamine, galactosamine, uronic acid, sialic acid, and several unknown kinds. Uronic acid or sialic acid was detected from 18 of the 43 K antigens tested.抄録全体を表示PDF形式でダウンロード (5252K) -
五十嵐 英夫, 太田 建爾, 善養寺 浩1969 年 24 巻 7 号 p. 338-344
発行日: 1969/07/25
公開日: 2009/02/19
ジャーナル フリーMany differential media have been devised for the identification of Shigellae, Salmonellae, and other enteric pathogens. None of them, however, had been designed for the determination of the activity of lysine decarboxylase, indole production, and motility of these organisms in a single tube of medium.
The present investigation was undertaken with an objective to reduce the man-hour and to simplify the conventional tedious procedures when a large number of samples were involved. The medium developed in it met those criteria and was named lysine-indole-motility (LIM) medium.
1) Composition and usage: LIM medium was composed of 10.0g of polypeptone (Daigo Eiyo Chemicals Co.), 3.0g of yeast extract (Difco), 1.0g of glucose, 10.0g of L-lysine monohydrochloride, 0.5g of L-tryptophan, 0.02g of bromcresol purple, 3.0g of agar, and 1, 000ml of distilled water. After these components were dissolved by heating at 100°C, pH was adjusted to 6.7±0.1. The resulting medium was dispensed into 10×100mm tubes in 3∼4ml amounts and sterilized at 121°C for 15 minutes. After the sterilization, the medium was cooled immediately by putting it into cold water. At use, organisms were picked up from a suspicious colony and stabbed into the middle of the medium. After 24 hours incubation at 37°C, reading was made.
2) Results of comparative study: Comparison of results was made between LIM medium and some known differential media. A total of 120 Salmonella strains, 166 Shigella strains, and 87 strains of the other enteric pathogen were used. There was a complete agreement on results. Therefore, the sensitivity and accuracy of the medium were demonstrated. Furthermore, in routine diagnostic use, all the enteric pathogens could be tested easily with the new medium in combination with triple sugar iron (TSI) agar.抄録全体を表示PDF形式でダウンロード (783K) -
ゴム腫生成機転に関する考察矢野 武1969 年 24 巻 7 号 p. 345-353
発行日: 1969/07/25
公開日: 2009/02/19
ジャーナル フリーIn experimental syphilis in rabbit, an exudative change which brings about destruction of the site of inoculation may be caused by the antigen-antibody complex.
In this investigation, an immunosuppressive agent was used to control the production of antibody.
On the other hand, multiple sensitization by the Treponema pallidum Nichols strain was repeated.
As a result, a gumma and a granuloma which was likely to change into a gumma were produced. In the central region of the gumma, the presence of Treponema pallidum was proved.
In the sensitized rabbits, serum antibody was measured by VDRL, TPHA, and TPIA. To check the presence of localized antibody, the fluorescein isothiocyanate-labeled antigen technique was applied.抄録全体を表示PDF形式でダウンロード (5074K)
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