巻号一覧
24 巻, 8 号
選択された号の論文の7件中1～7を表示しています
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I. 培地基材の毒素産生促進効果と感作原性山本 昭夫, 赤真 清人1969 年 24 巻 8 号 p. 359-364
発行日: 1969/08/25
公開日: 2009/02/19
ジャーナル フリーMost of the side reactions after the inoculation of tetanus toxoid are apparently due to allergic reaction, since they are always observed after repeated inoculations. The allergenic substances may be originated either from the culture medium or bacterial cells. In this paper, therefore, the allergenicities of peptone and heart infusion included in the medium for tetanus toxin production were studied. The allergenicity was examined by the appearance of the allergic reaction of immediate type caused by the intracutaneous injection of the substance in question in guinea pigs previously sensitized with the substances.
It was proved that heart infusions were more potent in the allergenicity than peptones. The minimum amounts of the former and the latter appearing the visible reaction were 0.7∼1.3mcg and 4∼20mcg respectively. On the other hand, heart infusion was essential for the consistent production of a potent tetanus toxin. No significant differences were observed between infusions derived from whale and beef heart in the allergenicity as well as the ability to promote the toxin production.
It was then examined to see whether the allergenicity and the ability to promote the toxin production were resulted from the same substances in the infusion. Heart infusion was fractionated by gel filtration through Sephadex G-75 and several fractions were tested for the effect on the toxin production. It was shown that the toxin production was promoted by the substances of small molecular weight in the infusion. The allergenicity was presumably due to the substances of large molecular weight. Details on the latter will be reported in the future paper.抄録全体を表示PDF形式でダウンロード (717K) -
第4報 ブイヨンと合成または半合成培地におけるP. multocidaの増殖の比較尾藤 行雄, 小畑 忠英1969 年 24 巻 8 号 p. 365-367
発行日: 1969/08/25
公開日: 2009/02/19
ジャーナル フリーActive growth arose in broth after inoculation with a very few cells. On the other hand, a much larger inoculum size was required for growth in a semi-synthetic medium containing protein digest. The strain used could not grow in the latter medium even after inoculation with a large number of cells. With the same inoculum size, much more active growth was observed in broth than in the semi-synthetic medium, regardless of the concentration of nutrients. A strain which had failed to grow in the semi-synthetic medium grew less actively in broth of a higher concentration than in the ordinary one. While one-step growth arose in the semi-synthetic medium, a growth curve of two-step type was observed in broth. A few synthetic media and many substances were examined for growthpromoting activity. As a result, no excellent synthetic media nor growth factors equivalent to broth were found.抄録全体を表示PDF形式でダウンロード (348K) -
第5報 馬肉水中からの増殖有効物質の分離尾藤 行雄, 小畑 忠英1969 年 24 巻 8 号 p. 368-372
発行日: 1969/08/25
公開日: 2009/02/19
ジャーナル フリーIt was presumed that at least three growth factors for Pasteurella multocida might be contained in broth prepared from horse meat. Various data suggested that one of them might be pyrimidine or its derivative. Broth prepared from horse meat seemed to contain many substances which might promote growth only when they were added to other nutrients.抄録全体を表示PDF形式でダウンロード (626K) -
宮城県における5年間の動態石田 名香雄, 山本 仁, 今野 二郎, 白取 剛彦, 遠藤 好喜1969 年 24 巻 8 号 p. 373-379
発行日: 1969/08/25
公開日: 2009/02/19
ジャーナル フリーThe five years survey results of antibody rise in summer against Japanese Encephalitis Virus in pigs in Natori district of Miyagi Prefecture were analyzed with regard to (1) the time of antibody rise, (2) the steepness of the antibody positive slope to reach to the height of 100%, (3) the level of antibody titers and (4) the detection rate of 2-ME sensitive antibodies. These serological results obtained in pigs were finally correlated to the isolation rate of the Japanese Encephalitis Virus from mosquitoes collected in the same area and to the incidence of Japanese Encephalitis patients among 1, 800, 000 residents in Miyagi Prefecture.
On the basis of this limited 5 years survey, one can conclude as follows:
1. A hundred percent infection among pigs and a consistent isolation of the virus from mosquitoes have been the case for these 5 years. Thus the cyclic outbreak between mosquitoes and pigs existed every year, even in the absence of the spread to human.
2. The time of initial and consistent isolation of virus from mosquitoes coincided completely with the time of antibody rise among pigs, as was pointed out by us 5 years ago.
3. The earlier the appearance of initial antibody rise among pigs, the higher the incidence of infection among human.
4. A minor pre-outbreak and a minor post-outbreak of infection among pigs were noticed before and after the main outbreak among pigs. The fact was clearly illustrated by pursuing the 2-ME sensitive antibodies.
5. The height of antibody titers in pig sera was assumed to be a reliable indicator to predict the amount of virus harbored by mosquitoes. The titer in pig sera was lowest in a year of the least incidence of human infections, irrespective of the fact that 100% of pigs were infected every year.抄録全体を表示PDF形式でダウンロード (906K) -
吉岡 一, 佐竹 良夫, 村山 隆志1969 年 24 巻 8 号 p. 380-386
発行日: 1969/08/25
公開日: 2009/02/19
ジャーナル フリーIncreasing drug resistance and lowering phage typability are the characteristic features of staphylococci isolated at the Hokkaido University Hospital in the recent years. Of 285 strains obtained in 1966, only 105 (36.9%) were typable by means of the routine typing phages, with an occurrence of 63 strains (22.1%) with types 80/81. Seventy-seven of 180 phages derived from the untypable staphylococci lyzed one or more of the standard propagating strains, 46 (25.5%) of which were either PS 80 or PS 81. Cross lysis among the untypable staphylococci revealed 54 strains (30.0%) to be lysogenic.
The phages from the untypable strains of staphylococci could be divided into the following three groups according to the host range. Two of the three groups were akin to each other and also to phages 80 and 81 (phages A and C); the third group was related to phage group III (phage B). Typing of the 180 untypable staphylococcal strains with these three phage varieties resulted in successful typing of 74 strains (41.1%), 70 of which were sensitive to the 80 and 81 related phages A and C. The fact suggests that a substantial portion of the untypable hospital strains of staphylococci may be lysogenized by phages related to phage 80 or 81 and hence phage resistant.
In the present study, a close relationship was observed between the phage types of staphylococci and their drug resistance. Strains typable with the routine typing phages, except phages 80 and 81, were generally less resistant to ordinary antibiotics. Type 80/81, as well as the untypable strains of staphylococci, was more resistant, while such strains as lyzed by phages A and/or C were extremely resistant. The high and multiple drug resistance of the latter strains might have occurred through transduction by some special phages.抄録全体を表示PDF形式でダウンロード (802K) -
Mycobacterium fortuitumとMycobacterium abscessusとの鑑別について斎藤 肇, 田坂 博信1969 年 24 巻 8 号 p. 387-393
発行日: 1969/08/25
公開日: 2009/02/19
ジャーナル フリーAn Adansonian classification was presented for sixteen strains of Mycobacterium fortuitum and fourteen strains of M. abscessus of Runyon's Group IV.
It was based on 65 characters determined by 24 tests.
The S-values among the strains of M. fortuitum were 82 to 100 per cent, with the average of 93 per cent, and those among the strains of M. abscessus 85 to 100 per cent, with the average of 95 per cent.
M. abscessus appeared to be a distinct species, exhibiting S-values ranging from 49 to 71 per cent, or 57 per cent on an average, when compared with M. fortuitum.
It was easy to distinguish M. abscessus from M. fortuitum on the basis of acid production from fructose, utilization of sodium citrate, nitrate reductase, allantoinase, nicotinamidase, phosphatase stable against heating at 70°C, PABA degradation, growth at 42°C, iron uptake, 5 per cent NaCl tolerance, susceptibility to 0.15M sodium nitrite, and bluing or greening of water blue.抄録全体を表示PDF形式でダウンロード (714K) -
II. 培地組成,前処理および培養温度の検討室橋 豊穂, 吉田 幸之助1969 年 24 巻 8 号 p. 394-401
発行日: 1969/08/25
公開日: 2009/02/19
ジャーナル フリーBacterial suspensions prepared from the nodules of 3 cases of L-type patients were inoculated into 0.1% semi-liquid agar media of various base compositions for the primary isolation. Prior to the cultivation, the bacterial suspension was mixed well with an equal volume of 2% NaOH at 37°C for 60 minutes to eliminate contamination, and was neutralized with N-HCl to adjust its pH to 6.6∼6.8. In any of the media used, obvious increase was observed in number and size of bacterial masses, showing fairly strong resistance to the alkali treatment. It was revealed again that this organism could be cultivated in cell-free, semi-synthetic softagar (0.1%) media, and that the growth seemed to be better in the medium which contained both glycerol and glucose as carbon sources than those which contained glucose alone. With regard to the incubation temperature, it was demonstrated clearly that the adequate temperature for the growth was 37°C, and not 30°C or less.抄録全体を表示PDF形式でダウンロード (6656K)
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