The TB-MacConkey agar plate, prepared from commercial MacConkey agar medium supplemented with 0.4% tributyrin, was proved to be useful for detecting
Acinetobacter anitratus from contaminated chick embryos. Almost all the colonies showing lipolysis on this plate were those of
A. anitratus.
When this selective medium was used for bacterial detection from other materials, such as soil and water, some Gram-negative bacilli,
e.g., Pseudomonas, Alcaligenes, Flavobacterium and Aeromonas, also formed colonies surrounded with zones of opacity. They were easily discriminated from
A. anitratus by a set of screening tests; namely, O-F test, oxidase test, properties on KI medium and motility test.
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