All issues
Volume 35 (1980)
- Issue 6 Pages 721-
- Issue 5 Pages 673-
- Issue 4 Pages 581-
- Issue 3 Pages 517-
- Issue 2 Pages 405-
- Issue 1 Pages 36-
Volume 35, Issue 6
Displaying 1-8 of 8 articles from this issue
- |<
- <
- 1
- >
- >|
-
Cellular network in its induction and manifestationKazuhisa SAITO1980 Volume 35 Issue 6 Pages 721-737
Published: November 25, 1980
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (2734K) -
Katsushi YOKOTA, Keiichi EBINA, Osamu SAKAGUCHI1980 Volume 35 Issue 6 Pages 739-745
Published: November 25, 1980
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSInvestigation was made on the properties of a hemolytic toxin isolated from the culture filtrate of the Link strain of Aspergillus flavus. The molecular weight of this toxin was estimated to be approximately 10, 000 by gel filtration, and its isoelectric point around pH 4.5 by the ampholine-electrofocusing method. It was stable in a pH range from 4 to 8, and unstable below pH 3 and above pH 9. The preparation was stable to heat-treatment up to 38C at pH 6.0 for 1 hour. Most of its activity was lost after heating at about 55C under the same conditions.
The hemolytic activity was activated by the addition of cholesterol or G-strophanthin. It was inhibited slightly by gangliosides and ZnCl2, and remarkably by HgCl2 and iodine. The oxidative phosphorylation in vitro of renal mitochondria from mice was inhibited by a high concentration (500μg/0.1ml) of the hemolytic toxin. This toxin induced moderate azotemia in mice in which the NPN value was 145-160mg/dl 12-24 hours after intravenous injection with 1mg of the toxin.View full abstractDownload PDF (2044K) -
Masayoshi IMAGAWA, Haruyo KITAGAWA, Tomio ICHIKAWA, Masaomi KONDO1980 Volume 35 Issue 6 Pages 747-751
Published: November 25, 1980
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSBacillus megaterium QM B1551 spores were germinated by the addition of 5mM CdCl2.Ca and dipicolinic acid were released and the heat resistance was lost from them in the germination with glucose and KNO3. O.D. during the CdCl2-induced germination was less than a half of that during the germination with glucose and KNO3.
When spores were germinated by the addition of CdCl2, they failed to incorporate leucine and consume oxygen in the medium. These results suggest that the very first step in the process of germination may have occurred without any following step.View full abstractDownload PDF (593K) -
Hiroshi USHIODA, Akiyoshi TSUJI, Masatoshi OGAWA, Sachiko GOTO, Senzo ...1980 Volume 35 Issue 6 Pages 753-763
Published: November 25, 1980
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSGlucose fermentation is regarded as an important criterion for differentiation of Staphylococcus from Micrococcus. For this purpose, the ICSB standard method and the Evans and Kloos method are available. Neither of them, however, is necessarity satisfactory in simplicity, rapidity, or accuracy.
In the present investigation, these methods were improved. The glucose fermentation test medium (GF medium) used was formulated with the following composition: tryptone (Difco), 17.0g; soytone (Difco), 3.0g; glucose, 10.0g; sodium chloride, 2.5g; sodium thioglycolate, 0.5g; L-cystine, 0.25g; sodium sulfite, 0.1g; agar (Difco), 3.0g; brom cresol purple (BCP), 0.04g; deionized water, 1, 000ml; pH 7.0.
All the ingredients were dissolved by heating at 100°C. The medium was dispensed into test tubes (18×180mm) in 20-ml amounts. The tubes were autoclaved at 115°C for 15 minutes and cooled immediately to make a semisolid deep agar medium. Prior to use, the tubes of the medium were boiled for 10 minutes to expel dissolved oxygen, and cooled down to about 45°C. A loopful of tryptic soy broth culture of the test organisms or a colony on an agar plate separated by a loop or needle was inoculated into a tube of GF medium to make a homogeneous suspension. The medium was mixed gently by shaking, allowed to solidify at room tem perature, and incubated at 37°C. Glucose fermentation was read 48-72 hours later.
Positive glucose-fermentation is denoted by abundant growth in all parts of the medium accompanied with pH changes. Negative glucose-fermentation is denoted by growth only on the surface of the medium accompanied with pH changes.
The improved method was proved to be useful, since such mineral oil as used in the ICSB standard method was not required. The used tubes can be cleaned easily. The avoidance of sticking culture much shortened the incubation period till reading. Anaerobiosis was maintained, even when the medium was shaken vigorously before the time of inoculation. It was possible to distinguish anaerobically grown organisms clearly from aerobically grown ones. Tests performed on many strains confirmed the usefulness of GF medium. It was proved that the improved method was superior to the ICSB standard method in simplicity, rapidity, and accuracy.View full abstractDownload PDF (7027K) -
Masanori ISHIBASHI, Yoshio KINOSHITA, Selena Lam, Yoshifumi TAKEDA, To ...1980 Volume 35 Issue 6 Pages 765-766
Published: November 25, 1980
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (169K) -
1980 Volume 35 Issue 6 Pages 767-771
Published: November 25, 1980
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (753K) -
1980 Volume 35 Issue 6 Pages 772-782
Published: November 25, 1980
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (1855K) -
1980 Volume 35 Issue 6 Pages 783-799
Published: November 25, 1980
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (2825K)
- |<
- <
- 1
- >
- >|