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Volume 41 (1986)
- Issue 6 Pages 783-
- Issue 5 Pages 749-
- Issue 4 Pages 679-
- Issue 3 Pages 587-
- Issue 2 Pages 497-
- Issue 1 Pages 51-
Volume 41, Issue 4
Displaying 1-6 of 6 articles from this issue
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Toshihiko KOGA1986 Volume 41 Issue 4 Pages 679-691
Published: July 25, 1986
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (8150K) -
Haruo WATANABE1986 Volume 41 Issue 4 Pages 693-699
Published: July 25, 1986
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (2475K) -
Hiroyuki YAMAGUCHI, Haruhiko TAGUCHI, Narihiro ISHIYAMA, Masato KANAMO ...1986 Volume 41 Issue 4 Pages 701-707
Published: July 25, 1986
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDistribution of cross-reacting protein antigen (CRPA) among 11 bacterial species was examined by immunoelectrophoresis. CRPA was detected in the sonicates of Shigella sonnei, Proteus mirabilis, Salmonella enteritidis, Klebsiella pneumoniae, Serratia marcescens, Escherichia coli, Yersinia enterocolitica, Pseudomonas aeruginosa and Vibrio cholerae but not in those of Neisseria gonorrhoeae or Staphylococcus aureus. A major protein with a molecular weight of 60 kilodaltons was found to be shared by the above nine species by SDS-PAGE and immunoblotting. CRPAs of V. cholerae and V. enterocolitica were partially purified by a combination of starch gel electrophoresis and gel filtration. The molecular weights of both CRPAs were estimated at about 500 kilodaltons by gel filtration. The 60 kilodalton protein was found also in both CRPAs by SDS-PAGE and immunoblotting, therefore, it is a major antigenic component of CRPAs of the above nine gram-negative rods. On the other hand, the major protein was not found in the outer membrane preparation obtained from V. cholerae. The present study suggests that CRPA is distinct from such previously reported common antigens as the outer membrane protein of V. cholerae and Kunin's antigen of enteric bacteria in the immunological and physico-chemical properties.View full abstractDownload PDF (6163K) -
Hiroshi NAGASAKI, Shuji TANAKA1986 Volume 41 Issue 4 Pages 709-716
Published: July 25, 1986
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSPhosphoenolpyruvate: sugar phosphotransferase systems (PTSs) for 10 carbohydrates utilizable as carbon and energy sources were examined with a Lactobacillus casei subsp. casei strain, LCC1. In contrast to the wild-type strain, a 2-deoxy-D-glucose (2DG)-resistant mutant, LCC17, derived from strain LCC1 was defective in more or less constitutive PTS activities for 2DG, glucose, mannose and glucosamine, confirming an earlier observation (Chassy, B.M. and Thompson, J., J. Bacteriol. 154: 1204-1214, 1983) that a single constitutive PTS, mannose-PTS, was involved in these activities. The mutant grew slowly on glucose or mannose and very poorly on glucosamine as compared with the wild-type strain, while the growth on the other 7 carbohydrates was not impaired. Induction experiments indicated that strain LCC17 might possess separate PTSs for fructose, mannitol, trehalose and galactose, each of which was inducible by the respective substrate though the activity of the last one was very low. No PTS activity for ribose, gluconate or maltose was detected with strain LCC17 grown in the presence or absence of these sugars.View full abstractDownload PDF (851K) -
I. Analysis of Sero-Reactive PatternNobunao IKEWAKI, Kimiyoshi TSUJI1986 Volume 41 Issue 4 Pages 717-719
Published: July 25, 1986
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSMonoclonal antibody Sa3 was obtained by fusion of the spleen cells from the BALB/c mice immunized with Epstein-Barr virus (EBV)-transformed human B lymphoblastoid cell line, EBVSA (HLA-A24; B7; C-; DR1; DQ1; DP4) with mouse myeloma NS-1 cells. In the microcytotoxicity test, this antibody reacted with EBV-transformed B lymphoblastoid cell lines, Burkitt's lymphomas, Pre-B leukemia and peripheral B cells, but not with T cell leukemias, Myelomonocytes, human spleen cells, human bone marrow cells or peripheral T cells. By SDS gel electrophoresis, the monoclonal antibody Sa3 detected on the HLA class II molecules was found to consist of two subunits of α chain (31-34k mol. wt.) and β chain (28-29k mol. wt.). These results suggest that monoclonal antibody Sa3 reacts with human HLA class II antigens.View full abstractDownload PDF (1395K) -
Masami TAKAHASHI, Eiichi YOSHIDA, Shigemi TERAKUBO, Atsushi TERADA1986 Volume 41 Issue 4 Pages 721-726
Published: July 25, 1986
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDuring the course of investigations on biological properties of Acinetobacter calcoaceticus, strains Ac-0156 and Ac-0181, which grew diffusely on blood agar, were obtained. When strain Ac-0181 was cultured on soft-agar medium containing 0.5% agar, the surface colonies were large. On 0.3% soft-agar, root-like colonies were formed, looking as if the medium had been dug by the organisms. Such growth propertis were shown also by strain Ac-0156, being different from those of a strain of Proteus mirabilis used for control. Strain Ac-0234 did not show diffuse growth on blood agar plates, but localized growth around the inoculated site as did a strain of Klebsiella pneumoniae use for control.View full abstractDownload PDF (5758K)
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