Nippon Saikingaku Zasshi
Online ISSN : 1882-4110
Print ISSN : 0021-4930
ISSN-L : 0021-4930
Volume 42, Issue 5
Displaying 1-8 of 8 articles from this issue
  • Nobuo TANAKA
    1987 Volume 42 Issue 5 Pages 707-716
    Published: September 25, 1987
    Released on J-STAGE: February 19, 2009
    JOURNAL FREE ACCESS
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  • Tsuyoshi UCHIDA
    1987 Volume 42 Issue 5 Pages 717-723
    Published: September 25, 1987
    Released on J-STAGE: February 19, 2009
    JOURNAL FREE ACCESS
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  • Eiro TSUBURA
    1987 Volume 42 Issue 5 Pages 725-729
    Published: September 25, 1987
    Released on J-STAGE: February 19, 2009
    JOURNAL FREE ACCESS
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  • Susumu KITAYA, Shuichi MIYAZAKI
    1987 Volume 42 Issue 5 Pages 731-738
    Published: September 25, 1987
    Released on J-STAGE: February 19, 2009
    JOURNAL FREE ACCESS
    We examined supernatants of mouse trachea and lung homogenates for the effects on the growth of H. influenzae and S. pneumoniae and obtained the following results:
    H. influenzae grew in nutrient broth containing supernatant of mouse trachea homogenate and the same medium containing X and V factors; however, it didn't grow in the same medium containing supernatant of mouse lung homogenate or the mouse trachea homogenate treated with NADase. S. pneumoniae grew in nutrient broth containing either supernatant of mouse lung homogenate or V factor; however, it failed to grow in the same medium containing supernatant of the mouse trachea homogenate, X and V factors, X factor and supernatant of mouse lung homogenate, or supernatant of lung homogenate treated with NADase.
    Supernatant of mouse trachea homogenate may contain both X and V factors necessary for stimulating the growth of H. influenzae. The presence of X factor in it was the cause of inhibition of the growth of S. pneumoniae.
    Supernatant of mouse lung homogenate contained V factor, stimulating the growth of S. pneumoniae and H. influenzae, but not X factor. Some protain inhibiting the growth of H. influenzae may be present in supernatant of lung homogenate.
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  • Fujio SUZUKI, Hiroshi MAEDA
    1987 Volume 42 Issue 5 Pages 739-746
    Published: September 25, 1987
    Released on J-STAGE: February 19, 2009
    JOURNAL FREE ACCESS
    The second suppressive state (S-SupS) in interferon (IFN) response was demonstrated when mice with third-degree burns of apporoximately 30% of the body surface area (burned mice) and its spleen cells were stimulated with staphylococcal enterotoxin A (SEA), a gamma IFN inducer. The first suppressive state in IFN production, appearing 3 to 7 days after thermal injury, was mediated by the generation of splenic suppressor macrophages. The S-SupS was demonstrated approximately 3 weeks after thermal injury, it persisted almost 2 weeks, and gradually disappeared by 7 weeks. Splenic mononuclear cells (MNC) of mice during S-SupS inhibited IFN production when they were co-cultured with normal mouse splenic MNC in the presence of concanavalin A. This suppressor cell activity could not be removed from burned mouse splenic MNC by carbonyl iron treatment, by a technique of adherence to plastic surface, or by treatments with anti-mouse immunoglobulin or anti-Lyt 1.2 monoclonal antibody and complement. However, the suppressor cell activity was inactivated by anti-Thy 1.2 or anti-Lyt 2.2 monoclonal antibodies plus complement. These results suggest that two different cell populations exist at different time periods after burn injury, and the secondarily generated suppressor cell was a T cell possessing the Lyt 1-2+ phenotype.
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  • II Analysis of immunoglobulin-production system using a monoclonal antibody
    Nobunao IKEWAKI, Kimiyoshi TSUJI, Noboru YAMAURA
    1987 Volume 42 Issue 5 Pages 747-750
    Published: September 25, 1987
    Released on J-STAGE: February 19, 2009
    JOURNAL FREE ACCESS
    To investigate the role of HLA class II antigens in the immunoglobulin-production system, the monoclonal antibody Sa3 against HLA class II antigens was added to the immunoglobulin-production system and detection of immunoglobulin secreting cells was performed by plaque assay with Protein A-coated sheep red blood cells. The results are as follows;
    1. Sa3 manifested profound inhibitory effect on Pokeweed mitogen-(PWM) induced immunoglobulin synthesis.
    2. The B cells treated with Sa3 in co-culture T cells did not produce immunoglobulin on PWM-induced immunoglobulin synthesis.
    3. Sa3 did not give any influence on induction of immunoglobulin secretion in B cell-differentiation factor (BCDF)-responsive CESS cells.
    Thus, HLA class II antigens were proven to play an important role in immunoglobulin-production system.
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  • Youichi ONOUE, Takanori TAKAHASHI, Minoru MORI
    1987 Volume 42 Issue 5 Pages 751-755
    Published: September 25, 1987
    Released on J-STAGE: February 19, 2009
    JOURNAL FREE ACCESS
    Combined effects of pH (4.5-7.4) and water activity (aw, 0.99-0.88) on the growth and enterotoxin production by five Staphylococcal strains 100, S-6, 361, K858 and T7436, producing enterotoxins A, B, C, AB, and AC, respectively, were studied in Brain Heart Infusion broth for 48hr. At 35C, Staphylococcal enterotoxin A (SEA) was produced at aw>0.92 or at pH>4.5, but no SEA was produced at aw 0.92 and pH 5.3. SEB and SEC were produced at aw>0.96 and pH>5.3 and aw>0.96 and pH>4.5, respectively. No SEB was produced at aw 0.96 and pH 5.3. No growth occurred at aw<0.92 and pH 4.5. The enterotoxin production changed depending on the interaction of these factors. SEA production was much less affected by aw and pH than was SEB and SEC. The enterotoxin production by the strains K858 and T7436 producing respectively SEA plus SEB and SEA plus SEC were both affected.
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  • Katsuya INADA, Kouzou KUMAGAI, Shigekazu MATUI, Miyuki SUZUKI, Masao Y ...
    1987 Volume 42 Issue 5 Pages 757-762
    Published: September 25, 1987
    Released on J-STAGE: February 19, 2009
    JOURNAL FREE ACCESS
    Effects of β-lactam antibiotics (piperacillin, cefapirin, cefoperazone, cefbuperazone) on human T lymphocyte colony formation were investigated. Peripheral blood mononuclear cells from six normal volunteers were cultured with phytohemagglutinin-P (PHA) and each antibiotic in semi-solid agar for 7 days. These four antibiotics, especially cefbuperazone, increased the number of colonies of mononuclear cells from five volunteers.
    The effects of these antibiotics on PHA-induced and direct growth of the mononuclear cells were slight and variable among six volunteers. No relationship was observed between the effects on T lymphocyte colony formation and PHA-induced growth by four antibiotics.
    Cefbuperazone enhanced PHA-induced interleukin 2 production by mononuclear cells from two volunteers, in which enhanced colony formation by the drug was observed.
    These results suggest that cefbuperazone stimulates the immunological function of lymphocytes.
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