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Volume 43 (1988)
- Issue 6 Pages 951-
- Issue 5 Pages 899-
- Issue 4 Pages 799-
- Issue 3 Pages 621-
- Issue 2 Pages 537-
- Issue 1 Pages 55-
Volume 43, Issue 2
Displaying 1-8 of 8 articles from this issue
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Koichiro YAMAMOTO1988 Volume 43 Issue 2 Pages 537-546
Published: March 25, 1988
Released on J-STAGE: February 19, 2009
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Akira IMADA1988 Volume 43 Issue 2 Pages 547-558
Published: March 25, 1988
Released on J-STAGE: February 19, 2009
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Yuichi SHIOKAWA1988 Volume 43 Issue 2 Pages 559-564
Published: March 25, 1988
Released on J-STAGE: February 19, 2009
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The role of asialo GM1-positive cellsTadahiro KASHO1988 Volume 43 Issue 2 Pages 565-571
Published: March 25, 1988
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSThe suppressive effect of mouse recombinant interferon-beta (IFN β) on B cell differentiation of MRL/Mp-lpr/lpr (MRL/l) mouse, a model of autoimmune disease, was investigated. Purified spleen mononuclear cells were cultured in the presence of lipopolysaccharide, and IFN β was examined for the suppressive activity on differentiation of B cells to immunoglobulin-producing cells by highy sensitive reversed hemolytic plaque assay. IFN β (500-10, 000units/ml) suppressed more than 50% of PFCs of both young and old MRL/l mice. This activity was abrogated by administration of 20μl/mouse of anti asialo GM1 12h before cultivation of lymphocytes.
These results suggest that natural killer cells activated by IFN β suppressed differentiation or maturation of B cells stimulated LPS and might be important to clarify the regulation of immunoglobulin-producing cells in auto-immune diseases.View full abstractDownload PDF (556K) -
Asako MURAOKA, Shuji TANAKA1988 Volume 43 Issue 2 Pages 573-576
Published: March 25, 1988
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSPhosphoenolpyruvate (PEP)-dependent hydrolysis of o-nitrophenyl-β-D-galactoside (ONPG) was tested with toluene-treated whole cells of 15 staphylococcal strains which were positive in lactose fermentation. The results indicated that the PEP: lactose phosphotransferase system was present in all five strains of Staphylococcus epidermidis and a S. warneri strain as well as five strains of S. aureus, but probably absent in four S. saprophyticus strains. The ONPG-hydrolyzing system of the first three species was induced by lactose and galactose, while that of S. saprophyticus was induced only by lactose.View full abstractDownload PDF (410K) -
Nobunao IKEWAKI, Noboru YAMAURA1988 Volume 43 Issue 2 Pages 577-581
Published: March 25, 1988
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSBurkitt's lymphoma Raji cells were mutanized with ethylmethanesulfonate (EMS) and selected in the presence of 6-thioguanine (6-TG). The most rapidly growing 6-TG-resistant clone (NIR10-2 cells) was obtained. The characteristics of the NIR10-2 cells were as follows:
1 The NIR10-2 cells were deficient in the hypoxanthine-guanine phosphoribosyl transferase (HGPRT) activity, because this cell line was sensitive to HAT selective medium.
2 Enzyme-linked immunosorbent assay (ELISA) found that the NIR10-2 cells did not produce human Ig in the culture medium.
3 The NIR10-2 cells grew well in the new culture medium, GIT.
These results suggest that the NIR10-2 cells are suitable for human-human hybridoma production.View full abstractDownload PDF (462K) -
1988 Volume 43 Issue 2 Pages 583-596
Published: March 25, 1988
Released on J-STAGE: February 19, 2009
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1988 Volume 43 Issue 2 Pages 597-605
Published: March 25, 1988
Released on J-STAGE: February 19, 2009
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