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Volume 48 (1993)
- Issue 6 Pages 721-
- Issue 5 Pages 677-
- Issue 4 Pages 621-
- Issue 3 Pages 511-
- Issue 2 Pages 373-
- Issue 1 Pages 64-
Volume 48, Issue 2
Displaying 1-9 of 9 articles from this issue
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Hiroto SHINOMIYA1993 Volume 48 Issue 2 Pages 373-388
Published: March 25, 1993
Released on J-STAGE: February 19, 2009
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Toshiyuki MASUZAWA1993 Volume 48 Issue 2 Pages 389-397
Published: March 25, 1993
Released on J-STAGE: February 19, 2009
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Hidenori MATSUI, Hirofumi DANBARA1993 Volume 48 Issue 2 Pages 399-406
Published: March 25, 1993
Released on J-STAGE: February 19, 2009
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Mitsunobu NISHIMOTO1993 Volume 48 Issue 2 Pages 407-415
Published: March 25, 1993
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSICR mice were infected intranasally with Mycoplasma pulmonis isolated freshly from the lungs of the rats with pneumonia. We demonstrated with high reproducibility the expressions of messenger RNAs of cytokines, tumor necrosis factor alpha (TNF-α) and interferon-γ (IFN-γ) in the lung tissue of M. pulmonis-infected mice. Both the viable population of M. pulmonis in the lung tissue and the titers of the neutralizing antibody in the serum increased in 7 and 14 days, respectively, and reached their muxima in 35 days after infection. Macroscopical and microscopical lesions were evident in the lungs of the mice inoculated with M. pulmonis and sacrificed in 21 days after the inoculation. Microscopically, mild infiltration of mononuclear cells and neutrophils in peribronchial and perivascular spaces were observed. The alveolar septa were swollen with infiltration of these cells.
Next, mRNAs prepared from the lung tissues of M. pulmonis-infected and -uninfected mice were tested for the presence of messages specific to TNF-α and IFN-γ by the reverse transcriptase-polymerase chain reaction. The expression of the genes encoding TNF-α and IFN-γ was constitutively demonstrated from 24h through 35 days after the intranasal inoculation of M. pulmonis. Furthermore, cells of two types, adherent and nonadherent cells, in bronchoalveolar lavage fluids obtained from the mice 3 weeks after inoculation of M. pulmonis were found also to express the genes of TNF-α and IFN-γ.
These data suggest that these cytokines would play a role in both stimulation and inhibition in the development of pathological changes in mycoplasmal infection, affecting the inf lamatory responses.View full abstractDownload PDF (6506K) -
relationship between the production of spirosome and anaerobic glycolysisSeiken MATAYOSHI1993 Volume 48 Issue 2 Pages 417-427
Published: March 25, 1993
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSThe locality and the function of spirosome were studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and electron microscopy in Peptostreptococcus productus, Clostridium sp. HD-17, Lactobacillus fermentum, Eubacterium aerofaciens and Escherichia cali B. When the bacterial cells in phosphate buffered saline were disrupted by sonication and fractionated by differential centrifugation, the spirosome protein was found in the cytoplasmic fraction and not in the fraction of cell wall and cell membrane (the envelope fraction). The spirosome protein was found, however, in the envelope fraction when the bacterial cells were treated with a proper concentration of SDS. The spirosome protein in the envelope fraction disappeared after treatment with 2% Triton X-100 for 2h. These results suggest that the spirosome protein anchored to the cell membrane upon SDS treatment. So it is presumed that the locality of spirosome is close to the cell membrane. Spirosome production increased in parallel to the concentration of glucose in the medium in obligate and aerotolerant anaerobes as well as in facultative anaerobes. This result indicates that the spirosome production was induced during the process of anaerobic glycolysis. Fructose as the sole carbon source in the minimal medium induced the spirosome production by E. coli as did glucose, but sodium pyruvate did not induce it either under aerobic or anaerobic condition.View full abstractDownload PDF (10586K) -
Nobunao IKEWAKI, Takeshi SASAHARA, Hidekazu TAMAUCHI, Hidetoshi INOKO1993 Volume 48 Issue 2 Pages 429-433
Published: March 25, 1993
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSA mouse monoclonal IgG1 antibody, referred to as NI-58, has been produced. In immunofluorescence assay, this antibody reacted with myelomonocytes, EBV-B cells, Burkitt's lymphoma cells, T cell leukemia cells and peripheral blood mononuclear cells, but not with erythroid cells. The surface antigen on U937 cells recognized by NI-58 had a molecular size of 65kDa as determined by immunoblotting analysis. As a biological function, NI-58 strongly inhibited the homotypic cell adhesion of LPS-stimulated U937 cells. It was found that the antigen defined by NI-58 was distinct from CD54 (intercellular adhesion molecule-1) in it's pattern of cellular expression and molecular weight, suggesting that NI-58 recognizes a new adhesion molecule and inhibits the homotypic cell adhesion of LPS-stimulated U937 cells.View full abstractDownload PDF (4425K) -
1993 Volume 48 Issue 2 Pages 435-449
Published: March 25, 1993
Released on J-STAGE: February 19, 2009
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1993 Volume 48 Issue 2 Pages 450-465
Published: March 25, 1993
Released on J-STAGE: February 19, 2009
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1993 Volume 48 Issue 2 Pages 466-479
Published: March 25, 1993
Released on J-STAGE: February 19, 2009
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