Breeding Science
Online ISSN : 1347-3735
Print ISSN : 1344-7610
ISSN-L : 1344-7610
Volume 68, Issue 2
Displaying 1-18 of 18 articles from this issue
Cover
  • 2018 Volume 68 Issue 2 Pages cover
    Published: 2018
    Released on J-STAGE: May 24, 2018
    JOURNAL FREE ACCESS

    On the cover

    A new super-hard rice cultivar, ‘Chikushi-kona 85’ (center right), showed higher content of resistant starch than the normal non-glutinous rice cultivar, ‘Nishihomare’ (center left). Grain appearance of ‘Chikushi-kona 85’ (lower right) was opaque, which differed from the translucent appearance of normal non-glutinous rice cultivar, ‘Nishihomare’ (lower left). Consumption of processed cookies made of Chikushi-kona 85 flour (lower center) showed distinctive effect in controlling blood sugar levels. Thus, ‘Chikushi-kona 85’ may be a novel genetic source to develop new products made of rice, which may reduce calorie intake and provide additional health benefits (This issue, p. 278–283).

    (T. Wada: Fukuoka Agriculture and Forestry Research Center)

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Invited Review
  • Ryo Fujimoto, Kosuke Uezono, Sonoko Ishikura, Kenji Osabe, W. James Pe ...
    2018 Volume 68 Issue 2 Pages 145-158
    Published: 2018
    Released on J-STAGE: May 24, 2018
    Advance online publication: April 12, 2018
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    Heterosis or hybrid vigor is a phenomenon where hybrid progeny have superior performance compared to their parental inbred lines. This is important in the use of F1 hybrid cultivars in many crops and vegetables. However, the molecular mechanism of heterosis is not clearly understood. Gene interactions between the two genomes such as dominance, overdominance, and epistasis have been suggested to explain the increased biomass and yield. Genetic analyses of F1 hybrids in maize, rice, and canola have defined a large number of quantitative trait loci, which may contribute to heterosis. Recent molecular analyses of transcriptomes together with reference to the epigenome of the parents and hybrids have begun to uncover new facts about the generation of heterosis. These include the identification of gene expression changes in hybrids, which may be important for heterosis, the role of epigenetic processes in heterosis, and the development of stable high yielding lines.

Review
  • Kul Bhushan Saxena, Arbind K. Choudhary, Rachit K. Saxena, Rajeev K. V ...
    2018 Volume 68 Issue 2 Pages 159-167
    Published: 2018
    Released on J-STAGE: May 24, 2018
    Advance online publication: April 12, 2018
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    Pigeonpea [Cajanus cajan (L.) Millsp.] is an ideal pulse crop of rainfed tropics and sub-tropics due to its high nutritive value and ability to survive various biotic and abiotic stresses. Thus it has continued to be cultivated on marginal land mostly under rainfed situation where the risk of crop failure is very high. To have insurance against crop failures and harvest more food in time and space, most farmers grow pigeonpea as an intercrop with short-aged cereals and other crops. Presently, intercropping system accounts for over 70% of the pigeonpea area. However, yield of pigeonpea in this system is very low (400–500 kg/ha). The non-availability of improved cultivars adapted specifically to the intercropping environments is perhaps the major constraint that accounts for low yield. Considering the food and nutritional needs of the ever increasing population, productivity enhancement of this high-protein pulse is highly indispensable. In this review, the authors critically examine the technical difficulties encountered by breeders in developing high yielding cultivars for intercropping systems and discuss the strategies to overcome these constraints.

Research Papers
  • Yukari Masuta, Akira Kawabe, Kosuke Nozawa, Ken Naito, Atsushi Kato, H ...
    2018 Volume 68 Issue 2 Pages 168-176
    Published: 2018
    Released on J-STAGE: May 24, 2018
    Advance online publication: March 24, 2018
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    Supplementary material

    In plants, several transposable elements are conserved across species. We found a homolog of ONSEN, which is a heat-activated retrotransposon originally isolated from Arabidopsis thaliana, in Vigna. The ONSEN-like elements (VaONS) were detected in all the analyzed Japanese accessions of Vigna angularis (adzuki bean) by Southern blot analysis. However, VaONS sequences were observed to be polymorphic in the different accessions. Interestingly, extrachromosomal DNA (ecDNA) was detected in some accessions of adzuki bean, indicating the conserved heat-activation of VaONS. Furthermore, we successfully induced retrotransposition of VaONS in adzuki plant regenerated through callus. Findings of our study should provide a new tool for molecular breeding of adzuki bean.

  • Kenichiro Fujii, Takashi Sayama, Kyoko Takagi, Kazumasa Kosuge, Katsun ...
    2018 Volume 68 Issue 2 Pages 177-187
    Published: 2018
    Released on J-STAGE: May 24, 2018
    Advance online publication: March 24, 2018
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    Supplementary material

    Single seed weight (SSW), or seed size, is a seed yield components (SYC) in soybean, and it is suggested that the genetic factors regulating SSW are involved in the control of other SYCs. The quantitative trait loci (QTLs) for SSW and their effects on the other SYCs were investigated using a recombinant inbred line population derived from typical small- and large-seeded cultivars that were cultivated in two different environments. QTL analysis detected four environmentally stable QTLs for SSW, two of which coincided with the defined loci, qSw17-1 and Ln. The effects of the other loci, qSw12-1 and qSw13-1, were confirmed by analyzing residual heterozygous line progenies derived from the recombinant population. These four QTL regions were also involved in the control of an additional SYC, namely the large-seeded allele at each locus that reduced either the number of pods per plant or the number of ovules per pod. These results suggest the presence of at least two different regulatory mechanisms for SSW. Isolation of genes responsible for these QTLs provides an important tool in the understanding and utilization of SSW diversity for soybean breeding.

  • Constantine Busungu, Satoru Taura, Jun-Ichi Sakagami, Toyoaki Anai, Ka ...
    2018 Volume 68 Issue 2 Pages 188-199
    Published: 2018
    Released on J-STAGE: May 24, 2018
    Advance online publication: April 10, 2018
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    Improvement of resistance against rice bacterial blight (BB) disease is an important breeding strategy in breeding programs across the world, especially in Africa and southern Asia where BB is more prevalent. This report describes a high-resolution map and characterization of xa42 at XA42 locus, a rice BB resistance gene in XM14, a mutant line originating from IR24. The candidate gene region was narrowed down from 582 kb, which had been obtained in our previous study, to 57 kb. XM14 shows brown spots in its leaves like lesion mimic mutants. This line also shows a shorter stature than the original cultivar IR24. In XA42 gene segregating populations, homozygotes of xa42 allele were consistently resistant to the six Japanese Xanthomonas oryzae pv. oryzae races used for this study. They also showed brown spots and markedly short stature compared with the other genotypes, suggesting that xa42 gene exhibits pleiotropic effects.

  • Yosuke Mizuno, Utako Yamanouchi, Tomoki Hoshino, Yasunori Nonoue, Kazu ...
    2018 Volume 68 Issue 2 Pages 200-209
    Published: 2018
    Released on J-STAGE: May 24, 2018
    Advance online publication: March 27, 2018
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    Supplementary material

    Seed dormancy is important in rice breeding because it confers resistance to pre-harvest sprouting (PHS). To detect quantitative trait loci (QTLs) for pre-harvest sprouting resistance, we used chromosome segment substitution lines (CSSLs) derived from a cross between the Japanese upland rice cultivar ‘Owarihatamochi’ and the lowland rice cultivar ‘Koshihikari’. In the CSSLs, several chromosomal regions were associated with PHS resistance. Among these, the chromosome 9 segment from ‘Owarihatamochi’ had the greatest association with increased PHS resistance. Further QTL analysis using an advanced backcross population (BC4F2) derived from a ‘Koshihikari’ × ‘Owarihatamochi’ cross revealed two putative QTLs, here designated qSDR9.1 (Seed dormancy 9.1) and qSDR9.2, on chromosome 9. The ‘Owarihatamochi’ alleles of the two QTLs reduced germination. Further fine mapping revealed that qSDR9.1 and qSDR9.2 were located within 4.1-Mb and 2.3-Mb intervals (based on the ‘Nipponbare’ reference genome sequence) defined by the simple sequence repeat marker loci RM24039 and RM24260 and Indel_2 and RM24540, respectively. We thus identified two QTLs for PHS resistance in ‘Owarihatamochi’, even though resistance levels are relatively low in this cultivar. This unexpected finding suggests the advantages of using CSSLs for QTL detection.

  • Satoshi Okada, Akio Onogi, Ken Iijima, Kiyosumi Hori, Hiroyoshi Iwata, ...
    2018 Volume 68 Issue 2 Pages 210-218
    Published: 2018
    Released on J-STAGE: May 24, 2018
    Advance online publication: March 30, 2018
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    Supplementary material

    Grain size is important for brewing-rice cultivars, but the genetic basis for this trait is still unclear. This paper aims to identify QTLs for grain size using novel chromosomal segment substitution lines (CSSLs) harboring chromosomal segments from Yamadanishiki, an excellent sake-brewing rice, in the genetic background of Koshihikari, a cooking cultivar. We developed a set of 49 CSSLs. Grain length (GL), grain width (GWh), grain thickness (GT), 100-grain weight (GWt) and days to heading (DTH) were evaluated, and a CSSL-QTL analysis was conducted. Eighteen QTLs for grain size and DTH were identified. Seven (qGL11, qGWh5, qGWh10, qGWt6-2, qGWt10-2, qDTH3, and qDTH6) that were detected in F2 and recombinant inbred lines (RILs) from Koshihikari/Yamadanishiki were validated, suggesting that they are important for large grain size and heading date in Yamadanishiki. Additionally, QTL reanalysis for GWt showed that qGWt10-2 was only detected in early-flowering RILs, while qGWt5 (in the same region as qGWh5) was only detected in late-flowering RILs, suggesting that these QTLs show different responses to the environment. Our study revealed that grain size in the Yamadanishiki cultivar is determined by a complex genetic mechanism. These findings could be useful for the breeding of both cooking and brewing rice.

  • Konana Arjun, Major S Dhaliwal, Salesh K Jindal, Bashasab Fakrudin
    2018 Volume 68 Issue 2 Pages 219-226
    Published: 2018
    Released on J-STAGE: May 24, 2018
    Advance online publication: April 07, 2018
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    Supplementary material

    Fruit length in chilli is quantitatively inherited trait and selection based on phenotypic performance is tedious and time consuming. To detect QTLs determining fruit length in Capsicum spp., an interspecific F2 mapping population was developed from the cross of C. annuum L. cv. ‘FL 201’ with C. galapagoense Hunz. accession ‘TC 07245’. Fruit length in this cross showed a quantitative inheritance with the population depicting a symmetric distribution in histogram. To map quantitative trait loci (QTLs) for fruit length 400 SSR markers were surveyed on the parental genotypes but only 28 markers were observed to be polymorphic indicating less genetic diversity between the two Capsicum species. Polymorphic markers were then analyzed in F2 population consisting of 210 plants and 24 of these markers were mapped on to three linkage groups (LGs): LG 1, LG 2 and LG 3. Two fruit length determining QTLs designated as paufl2.1 and paufl2.2 were identified and both the QTLs were mapped on to LG 2. The two QTLs together explained 21.78 per cent of the phenotypic variation. Apart from the two QTLs, positive alleles were detected in the small fruited parent ‘TC 07245’ which might be of potential use in chilli breeding programs.

  • Xia Chen, Hang-Gui Lai, Qi Sun, Jin-Ping Liu, Song-Bi Chen, Wen-Li Zhu
    2018 Volume 68 Issue 2 Pages 227-232
    Published: 2018
    Released on J-STAGE: May 24, 2018
    Advance online publication: April 04, 2018
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    Apomixis, or asexual seed formation, is of great value for plant breeding and seed production, and is desirable in modern agriculture, but natural apomixis occurs in cassava at very low frequency. In present study, apomixis was induced by the treatments of female flower buds with 1%, 1.5% and 2% (v/v) dimethyl sulfoxide (DMSO) and the results showed that 1.5% DMSO treatment was most effective for the induction of apomictic seed formation in cassava cultivar SC5 with the highest percentages of fruit set and true apomictic seeds. The germinated seedlings resembled their parents and displayed no morphological characteristics of cassava polyploid. Flow cytometry and chromosome counting showed that these plants were uniform diploids. Analysis of 34 DMSO-induced cassava progenies by the expressed sequence tag-simple sequence repeat (EST-SSR) and sequence-related amplified polymorphism (SRAP) markers showed that three true apomictic seeds were obtained from the group of SC5 treated with 1.5% DMSO.

  • Ning Li, Yanxu Yin, Fei Wang, Minghua Yao
    2018 Volume 68 Issue 2 Pages 233-241
    Published: 2018
    Released on J-STAGE: May 24, 2018
    Advance online publication: March 31, 2018
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    Supplementary material

    Pepper (Capsicum) is one of the most important vegetable and spice crops. Aphid-transmitted cucumber mosaic virus (CMV) causes significant damage to pepper crops across the world. The genetic basis of CMV resistance in pepper is complex, and the mechanisms underlying resistance remain largely unknown. Here, we employed a SLAF-seq approach to generate a high-density genetic map of pepper. The map spanned 1,785.46 cM, containing 12,727 markers on 12 chromosomes, with a mean marker distance of 0.16 cM between adjacent markers. We used this map and the interval mapping (IM) and multiple QTL mapping (MQM) procedures to detect genetic regions associated with quantitative trait for CMV resistance. Three QTLs, qcmv11.1, qcmv11.2 and qcmv12.1, conferred resistance to CMV and showed trait variation of 10.2%, 19.2% and 7.3% respectively. Our results will help to develop markers linked to CMV-resistant QTLs to improve pepper resistance to CMV.

  • Yu Zhang, Jiawu Zhou, Jing Li, Ying Yang, Peng Xu, Dayun Tao
    2018 Volume 68 Issue 2 Pages 242-247
    Published: 2018
    Released on J-STAGE: May 24, 2018
    Advance online publication: April 12, 2018
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    Oryza glumaepatula originates from South America continent and contains many valuable traits, such as tolerance to abiotic stress, high yield and good cooking qualities. However, hybrid sterility severely hindered the utilization of favorable genes of O. glumaepatula by interspecific hybridization. In order to further understand the nature of hybrid sterility between O. sativa and O. glumaepatula, a near isogenic line (NIL) was developed using a japonica variety Dianjingyou 1 as the recurrent parent and an accession of O. glumaepatula as the donor parent. A novel gene S56(t) for pollen sterility was mapped into the region between RM20797 and RM1093 on the short arm of chromosome 7, the physical distance between the two markers was about 469 kb. The genetic behavior of S56(t) followed one-locus allelic interaction model, the male gametes carrying the alleles of O. sativa in the heterozygotes were aborted completely. These results would help us clone S56(t) gene and understand the role of S56(t) in interspecific sterility.

  • Kiyomi Abe, Masao Oshima, Maiko Akasaka, Ken-ichi Konagaya, Yoshihiko ...
    2018 Volume 68 Issue 2 Pages 248-257
    Published: 2018
    Released on J-STAGE: May 24, 2018
    Advance online publication: March 24, 2018
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    Supplementary material

    Genomic selection is attracting attention in the field of crop breeding. To apply genomic selection effectively for autogamous (self-pollinating) crops, an efficient outcross system is desired. Since dominant male sterility is a powerful tool for easy and successive outcross of autogamous crops, we developed transgenic dominant male sterile rice (Oryza sativa L.) using the barnase gene that is expressed by the tapetum-specific promoter BoA9. Barnase-induced male sterile rice No. 10 (BMS10) was selected for its stable male sterility and normal growth characteristics. The BMS10 flowering habits, including heading date, flowering date, and daily flowering time of BMS10 tended to be delayed compared to wild type. When BMS10 and wild type were placed side-by-side and crossed under an open-pollinating condition, the seed-setting rate was <1.5%. When the clipping method was used to avoid the influence of late flowering habits, the seed-setting rate of BMS10 increased to a maximum of 86.4%. Although flowering synchronicity should be improved to increase the seed-setting rate, our results showed that this system can produce stable transgenic male sterility with normal female fertility in rice. The transgenic male sterile rice would promote a genomic selection-based breeding system in rice.

  • Yaping Liu, Aixia Xu, Fenghao Liang, Xueqin Yao, Yang Wang, Xia Liu, Y ...
    2018 Volume 68 Issue 2 Pages 258-267
    Published: 2018
    Released on J-STAGE: May 24, 2018
    Advance online publication: April 11, 2018
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    Clubroot is an economically important disease affecting plants in the family Cruciferae worldwide. In this study, a collection of 50 Cruciferae accessions was screened using Plasmodiophora brassicae pathotype 4 in China. Eight of these demonstrated resistance, including three Chinese cabbages, two cabbages, one radish, one kale, and one Brassica juncea. The three clubroot-resistant Chinese cabbages (1003, 1007 and 1008) were then used to transfer the clubroot resistance genes to B. napus by distant hybridization combined with embryo rescue. Three methods including morphological identification, cytology identification, and molecular marker-assisted selection were used to determine hybrid authenticity, and 0, 2, and 4 false hybrids were identified by these three methods, respectively. In total, 297 true hybrids were identified. Clubroot resistance markers and artificial inoculation were utilized to determine the source of clubroot resistance in the true hybrids. As a result, two simple sequence repeat (SSR) and two intron polymorphic (IP) markers linked to clubroot resistance genes were identified, the clubroot resistance genes of 1007 and 1008 were mapped to A03. At last, 159 clubroot-resistant hybrids were obtained by clubroot resistance markers and artificial inoculation. These intermediate varieties will be used as the ‘bridge material’ of clubroot resistance for further B. napus breeding.

  • Zhigang Liu, Xiangsheng Liu, Eric J. Craft, Lixing Yuan, Lingyun Cheng ...
    2018 Volume 68 Issue 2 Pages 268-277
    Published: 2018
    Released on J-STAGE: May 24, 2018
    Advance online publication: April 11, 2018
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    Supplementary material

    Root system architecture (RSA) plays an important role in the acquisition of mineral nutrients. Nevertheless, RSA has seldom been selected as an important agricultural trait in conventional breeding programs. Little is known about the response of RSA and phosphorus use efficiency (PUE) in regards to phosphorus (P) availability between parental inbred lines and their F1. In this study, 6 maize inbred lines and their 15 F1 generated by a diallel mating system, were used to analyze the genetic associations between RSA and PUE. Heterosis for PUE-related traits were comparatively greater under high P condition and reduced significantly under low P condition. Relative mid-parent heterosis for root traits were expressed more under the low P condition. Low P supply had a significant effect on heterosis, GCA and SCA of RSA- and PUE- related traits. The hybrid C3 (7922 × 8703-2), which had the highest PUE, showed an average yield with a lower P uptake under the both P conditions. Results from this study suggested breeding for a relatively high grain yield with reducing aboveground P demand and grain P concentration should be sufficient to reduce P fertilizer input and improve P efficiency.

Notes
  • Takuya Wada, Osamu Yamaguchi, Masayuki Miyazaki, Katsunori Miyahara, M ...
    2018 Volume 68 Issue 2 Pages 278-283
    Published: 2018
    Released on J-STAGE: May 24, 2018
    Advance online publication: April 12, 2018
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    Supplementary material

    A new super-hard rice cultivar, ‘Chikushi-kona 85’, which was derived from a cross between ‘Fukei 2032’ and ‘EM129’, was developed via bulk method breeding. ‘Chikushi-kona 85’ showed a higher content of resistant starch than the normal non-glutinous rice cultivar, ‘Nishihomare’, and a higher grain yield than the first super-hard rice cultivar, ‘EM10’. The amylopectin chain length of ‘Chikushi-kona 85’ and its progenitor line ‘EM129’ was longer than that of ‘Nishihomare’, and was similar to that of ‘EM10’. This suggests that the starch property of ‘Chikushi-kona 85’ was inherited from ‘EM129’, which is a mutant line deficient in a starch branching enzyme similar to ‘EM10’. Genetic analysis of ‘Chikushi-kona 85’ crossed with ‘Nishihomare’ also showed that the starch property of ‘Chikushi-kona 85’ was regulated by a single recessive gene. Consumption of processed cookies made from ‘Chikushi-kona 85’ flour showed a distinctive effect in controlling blood sugar levels in comparison to the normal non-glutinous rice cultivar ‘Hinohikari’. These results show that ‘Chikushi-kona 85’ is a novel genetic source to develop new products made of rice, which could reduce calorie intake and contribute to additional health benefits.

  • Masaki Fujimatsu, Hirokazu Hashizume, Tetsuo Fudan, Yasushi Koma, Rena ...
    2018 Volume 68 Issue 2 Pages 284-288
    Published: 2018
    Released on J-STAGE: May 24, 2018
    Advance online publication: April 12, 2018
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    ‘Harimaru’ is a new potato variety bred from a cross between ‘Saikai 35’ as a female parent and ‘Pike’ as a male parent. Marker selection was performed for 1,647 seedlings to combine resistance genes to late blight (R1), Potato virus Y (Rychc), Potato virus X (Rx1), and golden cyst nematode (H1). In total, 194 selected clones were evaluated in the field, among which the best clone was officially released as ‘Harimaru’. Its yield was slightly lower than the local standard variety, ‘May Queen’. However, it produces tasty potatoes, that do not become mushy with long boiling times despite its high starch content. ‘Harimaru’ may become a local specialty potato and its multiple resistance to potato viruses may allow cultivation using homemade seed tubers from the previous season’s crop.

  • Xuye Du, Xin Ma, Jingzhi Min, Xiaocun Zhang, Zhenzhen Jia
    2018 Volume 68 Issue 2 Pages 289-293
    Published: 2018
    Released on J-STAGE: May 24, 2018
    Advance online publication: April 12, 2018
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    Supplementary material

    A wheat-Aegilops searsii substitution line GL1402, in which chromosome 1B was substituted with 1Ss from Ae. searsii, was developed and detected using SDS-PAGE and GISH. The SDS-PAGE analysis showed that the HMW-GS encoded by the Glu-B1 loci of Chinese Spring was replaced by the HMW-GS encoded by the Glu-1Ss loci of Ae. searsii. Glutenin macropolymer (GMP) investigation showed that GL1402 had a much higher GMP content than Chinese Spring did. A dough quality comparison of GL1402 and Chinese Spring indicated that GL1402 showed a significantly higher protein content and middle peak time (MPT), and a smaller right peak slope (RPS). Quality tests of Chinese steamed bread (CSB) showed that the GL1402 also produced good steamed bread quality. These results suggested that the substitution line is a valuable breeding material for improving the wheat processing quality.

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