We estimated the normal values of 15k inds of plasma protein in 166 cases of healthy Japanese individuals using the single radial immunodiffusion method. The normal values of each plasma portein of the persons aged from 20 to 60 were as follows: prealbumin 22±5mg/dl, albumin 4091±581mg/dl, α1-acid-glycoprotein 60±22mg/dl, α1-antitrypsin 253±73mg/dl, haptoglobin 152±83mg/dl, ceruloplasmin 37±8mg/dl, α2-macroglobulin 231±65mg/dl, α2HS-glycoprotein 36±8mg/dl,β1A-globulin 94±26mg/dl, transferrin 237±44mg/dl, hemopexin 47±14mg/dl,β2-glycoprotein (Type 1) 10.4±2.1mg/dl, IgM 157±58mg/dl, IgA 273±69mg/dl, IgG 1264±182mg/dl, The observations made on the increase and decrease of protein based on age differences were as follows: albumin, transferrin, α1-antitrypsin tended to decrease, while IgG, α2-macroglobulin and IgA tended to increase correspondingly with aging. Another observation based on the differences of sex showed that only albumin recorded a higher value in the male, while in the female α2-macroglobulin, β1A-globulin,β2-glycoprotein, IgM and IgG showed higher values. A racial comparison of plasma proteins between Japanese and caucasians was made, showing that the values of many proteins were generally higher in western peoples, especially significant were hemopexin, transferrin and β2-glycoprotein, among 15 kinds of plasma proteins with the exception of immunoglobulins.
A new automated enzymatic method for the determination of glucose In blood or plasma is presented. The procedure employs the cuprlc-histamine complex instead of peroxidase and is based on the measurement of indigo carmine discolored by hydrogen peroxide which is derived from the first enzymatic reaction between glucose and glucose oxidase. Bacause of a competition between chromogen and naturally occurring oxidizable substances, particularly uric acid, the present method employs glucose solutions containing 5mg/100ml of Uric acid as the standard solutions. Plasma glucose concentrations determined by this method do not differ statistically from those obtained by two reference methods of glucose (manual enzymatic method and automated alkaline ferricyanide method). Recovery experiments gave mean yield of 102% and intra-runprecision was about 1.4% (C. V.). Fasting blood glucose levels from 95 normal subjects aged 20-48 years were in range 63-100mg/100ml. Emphasis is given to the necessity of finding a more specific automated method other than the alkaline ferricyanide method which is still widely preference in clinical laboratories for glucose estimation.
This report presents a newly-modified procedure for the automated simultaneous determination of uric acid and creatin ine in serum. Uric acid was assayed by photometrical measurements of colour produced with neocuproine. Neocuproine (less soluble in water) was dissolved in ethanol, and ethylene glycol was added to it (23.3vol%) so as to fit for an Auto-Analyzer analysis by increasing stability and viscosity. Jaffe's method with picric acid was applied to the determination of serum creatinine. Among many reducing substances in Vivo, which were presumed to influence determinations, ascorbic acid and glutathione was shown to be a cause for notably higher-values. Establishment of normal range of serum uric acid values was tried on 104healthy persons, as there had been noreliable reports on it. A slightly higher value(5.4±2.5mg/dl) was obtained than other several reports, especially on the male. Coefficients of variation of this modified methods were±5%for uric acid and±3%for creatinine. These methods correlated highly to usual manual method (r=0.99respectively).
A new method was introduced for the determination of iron in the form of ferrioxamine excreted in urine after the injection of desferrioxamine to the patient having iron overload. Sodium hydroxide solution was added to dissociate iron from ferrioxamine, and iron was co-precipitated with magnesium carbonate powder. Iron in the precipitate was dissolved in hydrochloric acid and determined colorimetrically using thioglycolic acid and orthophenanthroline. This method does not require a respective control sample as the method of direct colorization of iron in urine, or time consuming procedures as the method of ashing. Unlike the method of ashing and atomic absorption, the contamination of hemoglobin iron does not occur with this method.