Japanese Journal of Clinical Immunology
Online ISSN : 1349-7413
Print ISSN : 0911-4300
ISSN-L : 0911-4300
Volume 16, Issue 5
Displaying 1-12 of 12 articles from this issue
  • [in Japanese], [in Japanese], [in Japanese]
    1993 Volume 16 Issue 5 Pages 329-337
    Published: October 31, 1993
    Released on J-STAGE: January 22, 2009
    JOURNAL FREE ACCESS
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  • Toshio Suzuki, Shinpei Nakazawa, Taijiro Mori, Akitoshi Kinoshita, Tos ...
    1993 Volume 16 Issue 5 Pages 338-346
    Published: October 31, 1993
    Released on J-STAGE: January 22, 2009
    JOURNAL FREE ACCESS
    A human leukemic cell line, KOPT-5, was established from peripheral blood mononuclear cells of a 13 year-old boy with T cell acute lymphocytic leukemia. KOPT-5 has T cell phenotype of CD 1 (-), CD 2 (+), CD 3 (+), CD 4 (-), CD 5 (+), CD 7 (+), CD 8 (-). IL-2 activity in the culture supernatant of KOPT-5 was examined. KOPT-5 produces no IL-2 activity constitutionally, but produces IL-2 activity when co-cultured with both phytohemagglutinin (PHA) and macrophage-like differentiated human promyelocytic leukemia cell line HL-60. This differentiation of HL-60 have been induced by phorbol 12-myristate 13-acetate (PMA). KOPT-5 produces no IL-2 activity when co-cultured with macrophage-like differentiated HL-60 only, or with both PHA and non-differentiated HL-60, and KOPT-5 produces lower level of IL-2 activity when stimulated with both PHA and PMA, but produces no IL-2 activity when stimulated with both PHA and IL-1. The T cell line, KOPT-5 will provide a useful model for elucidating the mechanism of the accessory function of macrophage in mitogen-induced T cell differentiation.
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  • Masatake Sugimoto
    1993 Volume 16 Issue 5 Pages 347-354
    Published: October 31, 1993
    Released on J-STAGE: January 22, 2009
    JOURNAL FREE ACCESS
    It is well known that anti-histone antibodies (AHA) are generated frequently in Caucasian populations receiving certain drugs. In Japanese patients, however, drug-induced lupus or autoantibodies are very rare, so that the study of AHA has not received much attention. Therefore, the precise prevalence of AHA in Japanese patients remains unknown. AHA in patients with various connective tissue diseases were detected by an ELISA method that employed purified histones as the antigen. The patients included 140 with systemic lupus erythematosus (SLE), 45 with rheumatoid arthritis (RA), 34 with mixed connective tissue disease (MCTD), 30 with polymyositis (PM)/dermatomyositis (DM), and 30 with progressive systemic sclerosis (PSS). The positivity rate of serum for total histone was 35.7% in SLE, 8.9% in RA, 17.6% in MCTD, 16.7% in PM/DM, and 30.0% in PSS. Both SLE and PSS showed significantly higher positivity rates than RA (p<0.005, and p<0.05) respectively. In the analysis of each subfraction, it was noted that the positivity rate was relatively higher for H 1 and H 2 B in SLE patients and for H 3 and H 4 in PSS patients. Furthermore, the positivity rate in PSS patients was higher than the previously reported rate for Caucasians. Among SLE patients, the positivity rate was particularly high for H 1, and significantly higher than that for MCTD (p<0.01). In contrast, the positivity rate was lower for RA and PM/DM patients than for patients with other diseases. On the basis of the mode of reaction for individual sera, patients with SLE, MCTD or PSS generally reacted with various subfractions, whereas those with RA or PM/DM reacted mostly with a single subfraction.
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  • Atsushi Suzuki, Yasuo Matsuoka, Shoichiro Irimajiri
    1993 Volume 16 Issue 5 Pages 355-360
    Published: October 31, 1993
    Released on J-STAGE: January 22, 2009
    JOURNAL FREE ACCESS
    The usefulness of the new assay kit for high avidity anti-DNA antibody measurement, named RECOMBIGEN anti DNA Hi-A Kit (Hi-A Kit) was studied. The thirty three sera, whose titers of anti-DNA antibody were higher than 20U/ml by standard anti-DNA antibody assay, were measured by using Hi-A Kit. Confidence variation of intra-assay, interassay, inter-lot were good value. The titers of anti-DNA antibody by the new kit in 14 inactive systemic lupus erythematosus (SLE) were within normal range in 11 sera and were low in 3 sera. On the contrary the titers were high in all 15 active SLE sera. Non SLE patients (4 patients) indicated low titers. Hi-A Kit revealed good sensitivity and specificity for the active SLE. In conclusion, our data suggest this new kit is a useful tool for the diagnosis and clinical management of SLE.
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  • Akira Nakamura, Katsuhiro Shimoda, Takao Saito, Michio Kobayashi
    1993 Volume 16 Issue 5 Pages 361-369
    Published: October 31, 1993
    Released on J-STAGE: January 22, 2009
    JOURNAL FREE ACCESS
    In ongoing studies on immunotherapy against peritoneal carcinomatosis, LAK cells were introduced into the peritoneal cavity of mice. Antitumor effects of i. p. treatments of OK-432 and rIL-2 given sequentially were studied. When OK-432 was given in a dose of 1 KE, the number of peritoneal exudate cells (PEC) and the ratio of asialo GM 1 (asGM 1)+ cells significantly increased in murine peritoneal cavity and a peak was reached on day 5 after treatment. The mice were then injected i. p. with rIL-2 at a dose of 5×104U. LAK activity of PEC against NK-resistant target cells, EL-4, on day 6 was significantly higher than that in case of PEC with OK-432 alone. In vivo pretreatment of the PEC with anti-asGM 1 antibody prior to rIL-2 eliminated the augmentation of LAK activity. Sequential injection of OK-432 and rIL-2 to mice bearing X 5563 plasmacytoma augmented the LAK activity of PEC and the survival rate of tumor bearing mice was increased. Thus, the in vivo induction of LAK cells by the combined sequential injection of OK-432 and rIL-2 may be given consideration for treating patients with peritoneal carcinomatosis.
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  • Kazuhiko Kato, Yoshifumi Tada, Takehito Mayumi, Yasuo Yamauchi, Takesh ...
    1993 Volume 16 Issue 5 Pages 370-375
    Published: October 31, 1993
    Released on J-STAGE: January 22, 2009
    JOURNAL FREE ACCESS
    A 19-year-old female who had been diagnosed as Behçet's disease because of oral aphthae, genital ulcers, erythema nodosum and iridocyclitis underwent bilateral tonsillectomy because of chronic tonsillitis with a hope of improvement of Behçet's disease.
    Although major manifestations disappeared, she complained of fever and abdominal pain and weakness of the left radial pulsation was noted, eight months after tonsillectomy.
    Erythrocyte sedimentation rate was 119mm/hr and C-reactive protein was 7.2mg/dl. Digital subtraction angiography showed narrowing of the left subclavian artery and slight narrowing of the upper part of the abdominal aorta and a part of the superior mesenteric artery. We, therefore, considered that vascular involvement associated with Behçet's disease (Vasculo-Behçet's disease) had developed and increased the dose of prednisolone up to 40mg/day. Subsequently, erythrocyte sedimentation rate and C-reactive protein were normalized and symptoms were relieved.
    Considering the clinical course of the patient, tonsillectomy probably triggered vascular involvement of Behçet's disease. Much attention should be paid to the effects of tonsillectomy on the course of Behçet's disease.
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  • Ryutaro Nibu, Akihiro Yachie, Masae Sakakibara, Kazuhide Ohta, Hidetos ...
    1993 Volume 16 Issue 5 Pages 376-382
    Published: October 31, 1993
    Released on J-STAGE: January 22, 2009
    JOURNAL FREE ACCESS
    Toxic shock syndrome (TSS) is an acute, multisystem illness characterized by high fever, circulatory failure, desquamative skin lesion and multiple organ damage. It is usually associated with a focus of infection with staphylococcus aureus and its exotoxins have been considered to be the major contributing agents in the pathogenesis of TSS. Recently, it has become clear that most of the staphylococcal exotoxins acts as superantigens, activating a large fraction of T cells at the same time. The production of various cytokines induced by these toxins have been implicated in the development of TSS.
    We recently experienced a case of TSS in a 15-year-old boy. The characteristic features of the illness developed shortly after the introduction of anti-leukemic chemotherapy for acute myelocytic leukemia. Rapid deterioration of the clinical conditions was associated with high fever, circulatory failure, and evidence of liver damage. Oliguria and increase of BUN were also evident. Generalized erythematous skin lesion appeared at the peak of the illness. The patient's clinical condition recovered after short period of supportive therapy and the characteristic desquamation appeared during the convalescence. Staphylococcus aureus was recovered from the swollen lymph node in the neck.
    Antibody titers against TSST-1 and SEB remained high during the illness and did not show any appreciable change. There was no expansion or deletion of T cells with a particular Vβ repertoire during the course of illness. Although no specific toxin was found to be responsible, the characteristic clinical pictures were diagnostic of TSS. Patient's altered defense and the chemotherapy might have changed the growth and toxin producing profiles of the preexisting bacteria.
    Serum cytokine levels were measured during the acute stage of the illness. Not only monokine like IL-1β, TNFα and IL-6, but also IFNγ levels were markedly elevated during the acute stage of the illness. These cytokine levels returned quickly to normal values within a few days. The data indicated that the excessive production of the T cell derived cytokines, such as IL-2 and IFN-γ, play major roles in the development of the characteristic clinical picture of TSS.
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  • Junko Taguchi, Shigeto Kobayashi, Sung-wook Yang, Takao Hirano, Toshiy ...
    1993 Volume 16 Issue 5 Pages 383-387
    Published: October 31, 1993
    Released on J-STAGE: January 22, 2009
    JOURNAL FREE ACCESS
    Our case described a 62-year old male patient diagnosed as esophageal cancer with production of granulocyte colony-stimulating factor (G-CSF). A peripheral blood analysis revealed granulocytosis of 35, 400/mm3 although he did not have any infections, hematological disorders or metastasis of cancer. Serological analysis of G-CSF was not detected, though high concentration of G-CSF was determined in culture supernatant of carcinoma tissue resected from the esophagus. After total resection of cancer, the number of granulocyte normalized. Only one case of esophageal cancer suspected of G-CSF production has been reported, but there was no evidence that the cancer produced G-CSF. In this case, the production of G-CSF from esophageal cancer was demonstrated using monoclonal anti G-CSF antibodies in the enzyme linked immunoadsorbent assay.
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  • Minoru Nakamura, Masaru Kinoshita, Yoshihiro Tsuchiya, Hisayo Nonaka, ...
    1993 Volume 16 Issue 5 Pages 388-396
    Published: October 31, 1993
    Released on J-STAGE: January 22, 2009
    JOURNAL FREE ACCESS
    We report a 68 year-old man with overlap syndrome of progressive systemic sclerosis (PSS) and polymyositis (PM), who died of fetal hyperinfection with strongyloidiasis. In 1978, he developed muscle weakness and Raynaud's phenomenon. In 1982, he developed fever of 37_??_38°C and erythema with desquamation. He was diagnosed as erythroderma in the department of dermatology of our hospital in March 1987. He started receiving prednisolone at a daily dose of 5mg. However, in December 1987, he admitted to our hospital complaining of general fatigue. On admission, he revealed marked systemic scleroderma, pigmentation of the skin, muscle atrophy and weakness. Eosinophils were markedly increased in the peripheral blood. Serum IgE and CPK were also markedly elevated. Anti-nuclear antibodies were strongly positive. He was diagnosed as overlap syndrome with PSS and PM by biopsy of the skin and the muscle. Subsequently, treatment with 60mg/day of prednisolone was initiated. However, he developed abdominal pain, diarrhea, cough, hemosputum, followed by rapid progression of anemia and unconsciousness, and died in March 1988. Autopsy revealed the massive and diffuse bleeding in the bilateral lung, and the ulceration in the whole intestine with massive bleeding. Numerous filaria-form larva of strongyloides stercoralis were observed in the lung, intestine, liver, pancreas and mesenteric lymphnodes. In the literature, several cases of fetal hyperinfection with strongyloides stercoralis has been reported in immunocompromised host. However, there has been few reports concerning the hyperinfection of strongyloides stercoralis in patients with PSS or PM. Because of its poor prognosis, we should pay attention to the presence of strongyloidiasis in patients with collagen diseases when unexplained eosinophilia and elevated serum IgE were observed.
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  • Mitsuhiko Tanaka, Syuuhei Takai, Shigeto Kobayashi, Tomoko Matsumoto, ...
    1993 Volume 16 Issue 5 Pages 397-402
    Published: October 31, 1993
    Released on J-STAGE: January 22, 2009
    JOURNAL FREE ACCESS
    A case of lupus erythematosus profundus (LEP) which was improved by betamethasone was reported. A 47-year-old female admitted to our hospital with chief complaints of erythematosus skin lesions and subcutaneous nodules on the right cheek and chest. Light microscopic features of skin lesions were consistent with LEP, resulting from the dilatation of small vessels in the dermal layer, the infiltration of mononuclear cells mainly lymphocytes around the vessels and at the septum of subcutaneous tissue. Immunofluorescence staining revealed deposits of immunoglobulin at the epidermal-dermal junction. This finding supported the diagnosis of LEP and the patient improved remarkably by the administration of 1.25mg/day of betamethasone. From the review of previous literatures, the clinical and diagnosis of LEP are discussed.
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  • Hideo Okubo, Ryuji Shimamura, Yoshihiro Tsuchiya
    1993 Volume 16 Issue 5 Pages 403-408
    Published: October 31, 1993
    Released on J-STAGE: January 22, 2009
    JOURNAL FREE ACCESS
    A 50 year-old woman with malabsorption syndrome is herein reported. Before admission, she had complained of digestive organ disorders such as anorexia and caddy stool followed by pleural effusion, ascites and rhabdomyolysis, which was a result of hypoalbuminemia and hypokalemia. Diagnostic imaging-an endoscopic study, ultrasonography and an upper GI series-together revealed edematous changes in the esophagus, stomach and small intestines. Blood chemistry disclosed hypoalbuminemia, hypokalemia, as well as an increase in the LDH, CPK and aldolase levels in the serum. An absorption study showed malabsorption from the intestines. Autoantibodies such as anti-nuclear antibody and anti-DNA antibody were positive.
    While cases of SLE and PSS with malabsorption syndrome have been reported, this case was considered to be different from the previously reported cases. However, the presence of hyper-γ-globulinemia with autoantibodies and the dramatic effect of steroid therapy indicated that some autoimmune mechanism may have played a role in the malabsorption of the digestive organs in this case. An increase of plasmacytes with vacuoles and sea-blue histiocytes was also observed in the bone marrow smear.
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  • Tatsuya Atsumi, Atsushi Fujisaku, Nobutaka Ogura, Yoshiharu Amasaki, K ...
    1993 Volume 16 Issue 5 Pages 409-414
    Published: October 31, 1993
    Released on J-STAGE: January 22, 2009
    JOURNAL FREE ACCESS
    Raynaud's phenomenon, one of symptoms of collagen diseases, is due to insufficiency of peripheral blood circulation. This insufficiency is caused not only by vasoconstriction but also by histological change of blood vessels. Various vasodilators and inactivators of platelet aggregation have been used for treatment of Raynaud's phenomenon in patients with collagen diseases.
    In this study, we investigated the effects of beraprost sodium (BPS), a derivative of prostacycline, on Raynaud's phenomenon in patients with collagen diseases. In order to examine whether if BPS improved the blood circulation of fingers, we measured the temperature of fingers with thermography before and after cold water loading test (immersing both hands for 30 seconds in water at 15°C). After administration of BPS for 6 to 12 weeks, higher temperature of fingers, more rapid recovery of finger temperature after cold water loading test and improvement of thermal differences between fingertips and MP joints were observed.
    We concluded that cold water loading test with thermography was the good method for evaluation of drugs for peripheral circulation and that BPS was effective drug for Raynaud's phenomenon in patients with collagen diseases.
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