We have reported an accuracy of 73% using cluster analysis on drug sensitivity using the SPSS
TM (Method S) compared to genotyping for methicillin-resistant
Staphylococcus aureus (MRSA). Here we studied the efficacy of cluster analysis using Excel
TM with free additional software (Method E) on multi-drug susceptibility compared to Method S. Subjects were MRSA strains first isolated in an individual. Cluster analysis was performed in (1) 71 strains using the same 11 drugs used in our previous study, (2) 70 strains using 15 drugs for the 2011 series, and (3) 70 strains using 13 drugs for the 2005 series suspected of cross infection. (1) Method E typed completely as did Method S. (2) Method E showed sensitivity, specificity, and accuracy of 97.6%, 92.9%, and 95.7% compared to Method S. (3) Method S typed four strains in one cluster separately from those of hospital personnel, indicating cross propagation rather than transmission
via the staff. Hand-to-hand infection control was enforced with success. Method E showed identical typing with Method S. Methods E and S allowed typing in about three days. Therefore, typing using Method E may have advantages in analyzing MRSA cross infection, enabling rapid intervention.
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