In Japan, foodborne illness caused by
Salmonella have decreased year by year recently, but
Salmonella is an important causative agent of potential risks associated with death. However, the standard method applying food hygiene is not fully established at present. Then, we evaluate the advanced method for detecting
Salmonella from foods which was improved the official method for pasteurized liquid egg established on Nov. 1998. Sensitivity of the method was tested by two kinds of
Salmonella strain (H
2S producing and non-H
2S producing strain). Both kinds of
Salmonella strains inoculated in foods (3 or 7 cfu/25 g sample) were clearly detected by the advanced method. Moreover, practical use for detection of
Salmonella in ground chicken meat and unpasteurized whole liquid egg was tested. Retailed ground chicken meat was naturally contaminated by
Salmonella at a ratio of 40/70 at Tokyo and Osaka. However, concentration of
Salmonella was not so high. Mean of MPN (most probable number)/g was under 0.33/g. On the other hand, unpasteurized whole liquid egg was naturally contaminated by
Salmonella. It was shown higher MPN number than that of ground chicken meat. Ground chicken meat was mainly contaminated by
S. Infantis and non-sterilized liquid whole egg was mainly contaminated by
S. Enteritidis. Food causative agent of
Salmonella is mainly
S. Enteritidis in Japan. Egg contamination of
Salmonella needs to be monitored now and in the future. PCR and LAMP detection after BPW enrichment was fully effective for
Salmonella in non-sterilized liquid whole egg but that was less effective for
Salmonella in chicken ground meat. The new advanced detection methods for
Salmonella in food are useful and sufficient for detecting few
Salmonella in food.
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