Listeria monocytogenes has an ability to form biofilm which prevent the pathogen from being cleaned or sanitized and might become a contamination source to the products and the equipment. However, the interactions between the resident species and the pathogen at the biofilm formation have not been explored completely. In the present study, Paracoccus sp., Flavobacterium sp. and Micrococcus sp. were co-cultured with L. monocytogenes to reveal the effect to biofilm formation on the stainless coupon. Additionally, we analyzed their Lewis-acid properties which indicate electrical interaction of the cell body. The results of the co-culture described that three strains prevent the attachment of the pathogen. And, the prevention effects showed a negative correlation with the electron accepting/donating. This study demonstrated that it can be evaluated the part of effects of the resident strains from Lewis-acid property at the point of L. monocytogenes attachments. We believe that this finding will be useful information to estimate the fixation of the pathogen in food processing environment.