The multiplex PCR binary typing (mP-BIT) is applied to know the relationship among 386 Campylobacter jejuni/coli isolates obtained from patient, bovine, and poultry. As the result of this, two major clusters were observed consist of patient and poultry isolates. One of these clusters contains 27 isolates of patients from 13 cases (11 of 13 cases are poultry related) and 12 poultry isolates. The isolates including this major cluster have 10 genes by mP-BIT. Another cluster contains 19 isolates of patients from 9 cases (7 of 9 cases are poultry related) and 19 poultry isolates. Though little bovine isolates form cluster with patient isolates, major cluster consists only bovine isolates each other. Consequently, human foodborne campylobacteriosis might be mainly related to poultry isolates, on the other hand, only a part of the bovine isolates are related to human foodborne campylobacteriosis. These 386 isolates are also classified by Penner serotyping. As the result of this, Penner O and L are major (both 11 of 159 strains) among patient isolates, Penner D is the major (24 of 91 strains) among bovine isolates, Penner O (11 of 76 strains) is the major among poultry isolates. Though, major isolates (243 of 386; 63%) are Penner serotyping untypeable.
Arcobacter and Campylobacter are recognized as foodborne pathogens and frequently detected from chicken meat. The purpose of this study was to determine the contamination level by Arcobacter and Campylobacter of chicken meat obtained from the poultry processing plant in Yamaguchi prefecture. Samples were collected from six lots (four poultry farm) from July to December 2018. The evaluation of antimicrobial susceptibility of the isolates also was provided following the methods for broth microdilution testing. A. butzleri and C. jejuni were isolated from all lots, A. cryaerophillus and C. coli were isolated from each one lot. There was no significant difference between in contamination level among different lots (poultry farm) or pre- and post-chiller process. A. butzleri isolates (n=64) were resistant to ampicillin, erythromycin, tetracycline, chloramphenicol, nalidixic acid, ciprofloxacin and ofloxacin. A cryaerophillus isolates (n=2) susceptible to all tested drugs. C. jejuni isolates (n=67) were resistant to nalidixic acid, ciprofloxacin and ofloxacin. C. coli isolates (n=15) were resistant to streptomycin, tetracycline, nalidixic acid, ciprofloxacin and ofloxacin. It became clear from this study that A. butzleri isolates were resistant to some antibiotics commonly used for the treatment of infectious bacterial diseases in human and livestock animals.